FastQCFastQC Report
Thu 2 Feb 2017
SRR4062677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055519
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA168821.599402758263944No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC124371.1782829110608146No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA54600.5172810721550252No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC52830.5005120703653843No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG44690.4233936101576571No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA38700.36664427641757275No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG38200.36190727026230696No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC35440.3357589962852398No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32840.3111265642778576No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28510.2701040909732558No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA24550.23258700222355067No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC21770.20624924800027286No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA21240.2012280214756911No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG20690.19601731470489872No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA19570.18540642091710335No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT16530.1566054234930873No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG16440.15575276238513944No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15970.1512999765991896No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG14950.14163648404244739No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13030.1234463804062267No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11170.10582471750863792No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10890.10317199406168907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATA6050.026.90882944
TCGTATA752.064029E-623.46643844
GTATTAC956.840273E-720.8498061
TATGCGT1401.8553692E-1020.42837339
AATACCG550.00448349319.9998075
AAAGTAC6600.019.9998053
TGTAGGA10350.019.981432
GTCCTAA5400.019.9703371
CTGTAGG10500.019.9121151
CACACCG1001.1232642E-619.7998095
AAAAGTA6600.019.6674082
CCGGTCC3000.019.066489
TCTAGCG2800.018.8569628
ATACCGT2700.018.740566
GTCCTAC9100.018.6222611
GTCCTAT3950.018.3865361
CTAGCGG3000.018.33315529
CGCAATA2900.018.2067236
GTAGGAC11250.018.1864913
AGTACTC7500.018.1864915
Produced by FastQC (version 0.11.5)