##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062677_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1055519
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178005322500116	32.0	32.0	32.0	32.0	32.0
2	31.408836790242525	32.0	32.0	32.0	32.0	32.0
3	31.488158905713682	32.0	32.0	32.0	32.0	32.0
4	31.595687998036986	32.0	32.0	32.0	32.0	32.0
5	31.543576193322906	32.0	32.0	32.0	32.0	32.0
6	35.120162687739395	36.0	36.0	36.0	36.0	36.0
7	35.12788116556879	36.0	36.0	36.0	36.0	36.0
8	35.08393216986146	36.0	36.0	36.0	36.0	36.0
9	35.17603946494568	36.0	36.0	36.0	36.0	36.0
10	35.086905114924505	36.0	36.0	36.0	36.0	36.0
11	35.191415786925674	36.0	36.0	36.0	36.0	36.0
12	35.11121353571087	36.0	36.0	36.0	36.0	36.0
13	35.14073929507664	36.0	36.0	36.0	36.0	36.0
14	35.09092683315033	36.0	36.0	36.0	36.0	36.0
15	35.070240327270284	36.0	36.0	36.0	36.0	36.0
16	35.0730872679696	36.0	36.0	36.0	36.0	36.0
17	35.046695511876145	36.0	36.0	36.0	36.0	36.0
18	35.05084418186693	36.0	36.0	36.0	36.0	36.0
19	35.02007637948725	36.0	36.0	36.0	36.0	36.0
20	35.00359728247431	36.0	36.0	36.0	36.0	36.0
21	34.94173008728408	36.0	36.0	36.0	36.0	36.0
22	34.91377985616555	36.0	36.0	36.0	32.0	36.0
23	34.82310313694022	36.0	36.0	36.0	32.0	36.0
24	34.816013733528244	36.0	36.0	36.0	32.0	36.0
25	34.784754229909645	36.0	36.0	36.0	32.0	36.0
26	34.7074339732397	36.0	36.0	36.0	32.0	36.0
27	34.68801035320065	36.0	36.0	36.0	32.0	36.0
28	34.60614351802289	36.0	36.0	36.0	32.0	36.0
29	34.59185860226107	36.0	36.0	36.0	32.0	36.0
30	34.557890478522886	36.0	36.0	36.0	32.0	36.0
31	34.498876855840585	36.0	36.0	36.0	32.0	36.0
32	34.450037374978564	36.0	36.0	36.0	32.0	36.0
33	34.39456324329548	36.0	36.0	36.0	32.0	36.0
34	34.343985281174476	36.0	36.0	36.0	32.0	36.0
35	34.22392112316311	36.0	36.0	36.0	32.0	36.0
36	34.15656563264138	36.0	36.0	36.0	32.0	36.0
37	34.067741082822764	36.0	36.0	36.0	32.0	36.0
38	33.931969012400536	36.0	36.0	36.0	32.0	36.0
39	33.73189018861811	36.0	36.0	36.0	32.0	36.0
40	33.54901332898792	36.0	36.0	36.0	21.0	36.0
41	33.59969834744803	36.0	36.0	36.0	27.0	36.0
42	33.352969487048554	36.0	36.0	36.0	21.0	36.0
43	33.364417883524595	36.0	36.0	36.0	21.0	36.0
44	33.39482851564017	36.0	36.0	36.0	21.0	36.0
45	33.053426797622784	36.0	36.0	36.0	14.0	36.0
46	33.21321643665344	36.0	36.0	36.0	14.0	36.0
47	33.07570872717592	36.0	36.0	36.0	14.0	36.0
48	33.068993547250216	36.0	36.0	36.0	14.0	36.0
49	33.15046152650971	36.0	36.0	36.0	14.0	36.0
50	32.44093000694445	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	17.0
21	42.0
22	194.0
23	532.0
24	1341.0
25	2986.0
26	6025.0
27	10862.0
28	17820.0
29	26169.0
30	37185.0
31	52032.0
32	75559.0
33	102123.0
34	175804.0
35	546822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.763480381706074	17.503579323590753	13.092980925118939	24.63995936958423
2	13.398081616565861	21.651450725362682	37.9669948610669	26.983472797004566
3	19.555059535088184	26.594960549951114	27.575622067773743	26.27435784718696
4	12.39516975583506	17.25999416399183	34.52387268407111	35.820963396102
5	13.039556843600161	37.82669947201329	33.68968251637346	15.444061168013082
6	33.82108706712054	33.46685374682976	17.97059077098565	14.741468415064057
7	28.250489333199436	31.07838994692653	21.84633516434585	18.824785555528187
8	28.51526121273042	33.15373763996669	20.66471565173152	17.666285495571373
9	27.123149843820908	16.00776490048971	18.35201450660765	38.51707074908173
10	15.951868227857574	27.653789273333786	32.20264154411242	24.19170095469622
11	35.89741160509664	21.74333195328554	21.003980032571654	21.355276409046166
12	23.729463894065383	24.38857093050907	29.524717224417564	22.357247951007988
13	31.783700719740715	20.323082767813748	24.535702341691625	23.357514170753912
14	24.915326014974625	19.732567580498316	24.478763527705325	30.873342876821734
15	25.97973129806285	26.326290668382097	25.677036604741367	22.016941428813695
16	25.630708684542864	25.053741334831493	26.682513531258085	22.633036449367562
17	23.688157200391466	25.66415194799904	28.221661571227045	22.426029280382448
18	25.913034251396706	22.45937780371552	30.35454596269702	21.27304198219075
19	24.159205092471097	28.749174576677444	25.505083281305215	21.58653704954624
20	24.071286258229364	23.04146112007458	30.724221923053967	22.16303069864209
21	24.49875369368055	26.298342332066028	23.672335599832877	25.530568374420547
22	23.261163465555807	27.21087919781643	25.08045804954719	24.447499287080575
23	21.65313935608928	26.06272364590311	25.537863363899653	26.746273634107958
24	22.510347989946176	28.18414448247734	25.601718206872636	23.703789320703844
25	25.57547519277247	24.180332139923582	24.367917583672106	25.876275083631846
26	21.69416182939388	25.087279338410774	27.033430947240173	26.185127884955172
27	25.716069535460758	26.44831594694174	25.90554978167139	21.93006473592612
28	23.935144701327022	23.259458143339913	26.369113204025695	26.436283951307367
29	22.57145536934911	26.34864933743495	28.420710569871314	22.65918472334463
30	25.66187818504451	24.097718752575748	27.635125469082034	22.605277593297703
31	22.523327386811605	24.993202396167195	26.57716251436497	25.90630770265623
32	22.458809362976886	26.47664324375023	26.566172660084753	24.49837473318813
33	21.778291058711403	25.60389722970406	27.332809736252972	25.285001975331568
34	21.6838351559754	27.164267057248615	28.562441794036868	22.589455992739115
35	24.94677973584559	25.7632501167672	26.876162342885344	22.41380780450186
36	23.269311116142866	26.8735096194384	26.979334336947037	22.8778449274717
37	25.21697856694195	26.125820567891246	26.55546702617385	22.101733838992953
38	23.869489796015042	26.511791829422304	24.464268288870215	25.154450085692442
39	23.322081364712524	25.696932030593477	27.205668491045635	23.775318113648357
40	25.777555875356107	25.535873821314443	24.676012464010597	24.010557839318857
41	22.00405677207137	25.81327290176681	27.584913203836216	24.597757122325607
42	26.807381013510888	26.450494969773164	25.76751342230694	20.974610594409008
43	24.856113438033802	23.857836760873084	25.232989647746752	26.053060153346365
44	23.858499941734824	25.143839191904643	28.396646578602564	22.601014287757966
45	25.24473742301181	25.36884698427977	26.151021440637262	23.235394152071162
46	23.106831805017247	24.73636192242868	28.65244491098692	23.504361361567152
47	25.938708824758244	23.718474039785168	28.235398889077317	22.10741824637927
48	23.305880803661516	29.708323582995668	24.414340243993713	22.57145536934911
49	22.681922352889906	24.94933771916943	29.768199340798223	22.60054058714244
50	22.383775185477475	29.806663830778984	23.16054945481796	24.64901152892558
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	60.0
2	65.0
3	250.0
4	435.0
5	458.0
6	481.0
7	426.5
8	372.0
9	468.5
10	565.0
11	882.5
12	1200.0
13	2052.0
14	2904.0
15	3941.0
16	4978.0
17	5441.0
18	5904.0
19	5849.0
20	5794.0
21	6225.5
22	6657.0
23	6915.5
24	7174.0
25	7113.0
26	7052.0
27	8717.5
28	10383.0
29	12386.5
30	14390.0
31	17034.5
32	19679.0
33	23682.0
34	27685.0
35	32400.0
36	37115.0
37	39674.5
38	42234.0
39	42486.0
40	42738.0
41	45157.0
42	47576.0
43	50790.5
44	54005.0
45	74736.0
46	95467.0
47	110646.0
48	125825.0
49	115045.5
50	104266.0
51	85716.5
52	67167.0
53	61068.0
54	54969.0
55	55625.5
56	56282.0
57	54776.0
58	53270.0
59	48753.0
60	44236.0
61	39190.5
62	34145.0
63	29301.5
64	24458.0
65	21066.5
66	17675.0
67	15118.0
68	12561.0
69	11421.5
70	10282.0
71	7887.5
72	5493.0
73	4906.0
74	4319.0
75	3012.5
76	1706.0
77	1543.5
78	1381.0
79	1174.5
80	968.0
81	766.5
82	565.0
83	532.0
84	499.0
85	407.0
86	315.0
87	237.5
88	160.0
89	96.5
90	33.0
91	23.5
92	14.0
93	11.0
94	8.0
95	8.5
96	9.0
97	7.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014400498712008027
2	0.00445278578594985
3	6.631808617372117E-4
4	2.842203693159479E-4
5	0.0
6	0.0
7	9.474012310531596E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1055519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.737735603880225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06235287918325	37.558445796385506
2	13.649669797037097	13.305079952965269
3	4.202618362997286	6.144783078593209
4	1.7234300283097883	3.359843082062011
5	0.9013921168129734	2.19659053323263
6	0.521275873014734	1.5243483405404366
7	0.33950925807311405	1.1582838718525879
8	0.22307221460619897	0.8697627692839163
9	0.16099374697025054	0.7061823606342655
>10	0.9996029713886764	9.165391482457462
>50	0.1020919462978723	3.5263378571400863
>100	0.09954676506801516	9.153224878830551
>500	0.009954676382131131	3.380006419223669
>1k	0.0037086049266763037	4.1405792118409845
>5k	3.903794659659267E-4	1.0219929344700218
>10k+	3.903794659659267E-4	2.7891474304874397
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	16882	1.599402758263944	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	12437	1.1782829110608146	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	5460	0.5172810721550252	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	5283	0.5005120703653843	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	4469	0.4233936101576571	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	3870	0.36664427641757275	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	3820	0.36190727026230696	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	3544	0.3357589962852398	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3284	0.3111265642778576	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2851	0.2701040909732558	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2455	0.23258700222355067	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	2177	0.20624924800027286	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	2124	0.2012280214756911	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	2069	0.19601731470489872	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	1957	0.18540642091710335	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	1653	0.1566054234930873	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1644	0.15575276238513944	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1597	0.1512999765991896	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	1495	0.14163648404244739	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1303	0.1234463804062267	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1117	0.10582471750863792	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	1089	0.10317199406168907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8948024621063191E-4
2	0.0	0.0	0.0	0.0	1.8948024621063191E-4
3	0.0	0.0	0.0	0.0	1.8948024621063191E-4
4	0.0	0.0	0.0	0.0	1.8948024621063191E-4
5	0.0	0.0	0.0	0.0	1.8948024621063191E-4
6	0.0	0.0	0.0	0.0	1.8948024621063191E-4
7	0.0	0.0	0.0	0.0	1.8948024621063191E-4
8	0.0	0.0	0.0	0.0	1.8948024621063191E-4
9	0.0	0.0	0.0	9.474012310531596E-5	2.842203693159479E-4
10	0.0	0.0	0.0	9.474012310531596E-5	2.842203693159479E-4
11	0.0	0.0	0.0	9.474012310531596E-5	2.842203693159479E-4
12	0.0	0.0	0.0	9.474012310531596E-5	4.737006155265798E-4
13	0.0	0.0	0.0	9.474012310531596E-5	7.579209848425277E-4
14	0.0	9.474012310531596E-5	0.0	9.474012310531596E-5	9.474012310531596E-4
15	0.0	9.474012310531596E-5	0.0	9.474012310531596E-5	0.0011368814772637916
16	0.0	9.474012310531596E-5	0.0	9.474012310531596E-5	0.0011368814772637916
17	0.0	9.474012310531596E-5	0.0	9.474012310531596E-5	0.0011368814772637916
18	0.0	9.474012310531596E-5	0.0	1.8948024621063191E-4	0.0012316216003691075
19	0.0	9.474012310531596E-5	0.0	2.842203693159479E-4	0.0013263617234744235
20	0.0	9.474012310531596E-5	0.0	5.684407386318958E-4	0.0016105820927903715
21	0.0	9.474012310531596E-5	0.0	7.579209848425277E-4	0.0016105820927903715
22	0.0	9.474012310531596E-5	0.0	0.0012316216003691075	0.0017053222158956874
23	0.0	9.474012310531596E-5	0.0	0.003221164185580743	0.0017053222158956874
24	0.0	9.474012310531596E-5	0.0	0.005494927140108326	0.0017053222158956874
25	0.0	9.474012310531596E-5	0.0	0.007200249356004013	0.0017053222158956874
26	0.0	9.474012310531596E-5	0.0	0.010705633910900705	0.0017053222158956874
27	0.0	9.474012310531596E-5	0.0	0.014400498712008027	0.0018948024621063193
28	0.0	9.474012310531596E-5	0.0	0.019421725236589773	0.0018948024621063193
29	0.0	9.474012310531596E-5	0.0	0.02861151717780542	0.0018948024621063193
30	0.0	9.474012310531596E-5	0.0	0.04196987453565497	0.0018948024621063193
31	0.0	9.474012310531596E-5	0.0	0.06679178678924776	0.0018948024621063193
32	0.0	9.474012310531596E-5	0.0	0.09919290889126581	0.001989542585211635
33	0.0	9.474012310531596E-5	0.0	0.12827812668459782	0.001989542585211635
34	0.0	9.474012310531596E-5	0.0	0.167311057403988	0.002273762954527583
35	0.0	9.474012310531596E-5	0.0	0.21155469489417056	0.002273762954527583
36	0.0	9.474012310531596E-5	0.0	0.27256733417399404	0.002273762954527583
37	0.0	9.474012310531596E-5	0.0	0.3716655029421545	0.002273762954527583
38	0.0	9.474012310531596E-5	0.0	0.48724845313064	0.002368503077632899
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTATA	605	0.0	26.908829	44
TCGTATA	75	2.064029E-6	23.466438	44
GTATTAC	95	6.840273E-7	20.849806	1
TATGCGT	140	1.8553692E-10	20.428373	39
AATACCG	55	0.004483493	19.999807	5
AAAGTAC	660	0.0	19.999805	3
TGTAGGA	1035	0.0	19.98143	2
GTCCTAA	540	0.0	19.970337	1
CTGTAGG	1050	0.0	19.912115	1
CACACCG	100	1.1232642E-6	19.799809	5
AAAAGTA	660	0.0	19.667408	2
CCGGTCC	300	0.0	19.06648	9
TCTAGCG	280	0.0	18.85696	28
ATACCGT	270	0.0	18.74056	6
GTCCTAC	910	0.0	18.622261	1
GTCCTAT	395	0.0	18.386536	1
CTAGCGG	300	0.0	18.333155	29
CGCAATA	290	0.0	18.20672	36
GTAGGAC	1125	0.0	18.186491	3
AGTACTC	750	0.0	18.186491	5
>>END_MODULE