FastQCFastQC Report
Thu 2 Feb 2017
SRR4062676_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062676_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188340
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG56983.025379632579378No Hit
TCCATGTACTCTGCGTTGATACCAC48142.5560157162578316No Hit
GTATCAACGCAGAGTACTTTTTTTT45472.4142508229797173No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38332.035149198258469No Hit
TATCAACGCAGAGTACTTTTTTTTT29941.5896782414781776No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27671.4691515344589572No Hit
GAGTACATGGAAGCAGTGGTATCAA27231.4457895295741743No Hit
CATGTACTCTGCGTTGATACCACTG24371.2939364978230858No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21001.1150047785919084No Hit
GGTATCAACGCAGAGTACTTTTTTT17030.9042157799723903No Hit
CCCATGTACTCTGCGTTGATACCAC15690.8330678560050973No Hit
GTACATGGGAAGCAGTGGTATCAAC15560.8261654454709567No Hit
GCTTCCATGTACTCTGCGTTGATAC14060.7465222470001062No Hit
GCGTTGATACCACTGCTTCCATGTA13950.7406817457789104No Hit
CATGGAAGCAGTGGTATCAACGCAG12290.6525432728045024No Hit
GTATCAACGCAGAGTACATGGAAGC10960.5819263034936817No Hit
ACTCTGCGTTGATACCACTGCTTCC10400.5521928427312308No Hit
GTATCAACGCAGAGTACATGGGAAG10050.5336094297546989No Hit
CAGTGGTATCAACGCAGAGTACATG9060.48104491876393757No Hit
GTACATGGGGTGGTATCAACGCAAA8780.46617818838271213No Hit
ACGCAGAGTACATGGAAGCAGTGGT8540.453435276627376No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8070.4284804077731762No Hit
GCGTTGATACCACTGCTTCCCATGT6520.3461824360199639No Hit
TATCAACGCAGAGTACATGGAAGCA6450.3424657534246575No Hit
TATCAACGCAGAGTACATGGGAAGC6220.33025379632579377No Hit
GAGTACATGGGAAGCAGTGGTATCA6020.31963470319634707No Hit
ATCAACGCAGAGTACTTTTTTTTTT5680.3015822448762876No Hit
GTATCAACGCAGAGTACATGGGGTG5490.29149410640331314No Hit
ATACCACTGCTTCCATGTACTCTGC5470.2904321970903685No Hit
GTACATGGGTGGTATCAACGCAAAA5350.2840607412127004No Hit
GGTATCAACGCAGAGTACATGGGAA5200.2760964213656154No Hit
GTACTCTGCGTTGATACCACTGCTT4720.25061059785494316No Hit
GGTATCAACGCAGAGTACATGGAAG4680.24848677922905382No Hit
AAGCAGTGGTATCAACGCAGAGTAC4500.23892959541255177No Hit
AAAAAGTACTCTGCGTTGATACCAC4440.23574386747371776No Hit
GTACATGGGAGTGGTATCAACGCAA4390.23308909419135607No Hit
CCACTGCTTCCATGTACTCTGCGTT4340.23043432090899435No Hit
ACGCAGAGTACATGGGAAGCAGTGG4130.2192842731230753No Hit
TATCAACGCAGAGTACATGGGGTGG3890.20654136136773918No Hit
GTGGTATCAACGCAGAGTACATGGA3700.1964532228947648No Hit
GTATTAGAGGCACTGCCTGCCCAGT3370.17893171923117768No Hit
GCTTCCCATGTACTCTGCGTTGATA3350.17786980991823298No Hit
GTATCAACGCAGAGTACATGGGCAG3310.17574599129234364No Hit
GAAGCAGTGGTATCAACGCAGAGTA3300.1752150366358713No Hit
CTGCTTCCATGTACTCTGCGTTGAT3200.16990549007114794No Hit
GTATCAACGCAGAGTACATGGGAGT3150.16725071678878625No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3060.1624721248805352No Hit
CCATAGGGTCTTCTCGTCTTATTAT3040.16141021556759053No Hit
ACATGGAAGCAGTGGTATCAACGCA2990.15875544228522884No Hit
GTTGATACCACTGCTTCCATGTACT2970.15769353297228417No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2840.1507911224381438No Hit
TGGAAGCAGTGGTATCAACGCAGAG2840.1507911224381438No Hit
GTGGTATCAACGCAGAGTACATGGG2800.14866730381225443No Hit
GATACCACTGCTTCCATGTACTCTG2800.14866730381225443No Hit
GGTATCAACGCAGAGTACATGGGGT2550.135393437400446No Hit
CTCTAATACTTGTAATGCTAGAGGT2530.13433152808750132No Hit
ACGCAGAGTACATGGGGTGGTATCA2530.13433152808750132No Hit
GCAGAGTACATGGAAGCAGTGGTAT2370.12583625358394393No Hit
CAACGCAGAGTACTTTTTTTTTTTT2310.12265052564510989No Hit
CTTCCATGTACTCTGCGTTGATACC2290.12158861633216525No Hit
GTATCAACGCAGAGTACATGGGTGG2270.12052670701922057No Hit
CCCCATGTACTCTGCGTTGATACCA2250.11946479770627588No Hit
CCATGTACTCTGCGTTGATACCACT2140.11362429648508017No Hit
GGTATCAACGCAGAGTACATGGGAG2110.11203143251566318No Hit
GCAGTGGTATCAACGCAGAGTACAT2110.11203143251566318No Hit
GATATATTTTGATCAACGGACCAAG2070.10990761388977383No Hit
AACGCAGAGTACTTTTTTTTTTTTT2010.10672188595093979No Hit
ATCAACGCAGAGTACATGGAAGCAG1990.10565997663799512No Hit
TCAACGCAGAGTACTTTTTTTTTTT1980.10512902198152277No Hit
CCATTGGGATGTCCTGATCCAACAT1970.10459806732505043No Hit
CTTCTACACCATTGGGATGTCCTGA1970.10459806732505043No Hit
GAGTACATGGGGTGGTATCAACGCA1950.10353615801210576No Hit
TGATACCACTGCTTCCATGTACTCT1950.10353615801210576No Hit
ATCCTGACCGTGCAAAGGTAGCATA1900.10088138472974409No Hit
TATCAACGCAGAGTACATGGGAGTG1900.10088138472974409No Hit
GTTCATGCTAGTCCCTAATTAAGGA1900.10088138472974409No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGCT307.6854345E-419.0010628
AATATGG307.6854345E-419.00106214
AATCGTA307.6854345E-419.00106213
TGGCTGC250.00601089519.00106210
TTATTGG250.00602012818.9960173
TTAGGAC250.00602012818.9960173
GACAATT350.002159878816.28662518
GCATTAC350.002159878816.2866257
ACAATTA350.002159878816.28662519
TCTAGCA350.002163716616.28233
AGGGTCC456.720506E-414.778604519
CGAGGGT456.720506E-414.778604517
GAGGGTC456.720506E-414.778604518
CCAACAC400.005254383714.2507969
TATCGAC400.005254383714.25079614
CATTACA400.005254383714.2507968
AGCATTA400.005254383714.2507966
CGGCTAA400.005254383714.2507969
ACGGCTA400.005254383714.2507968
GGGTGTG400.005254383714.2507967
Produced by FastQC (version 0.11.5)