FastQCFastQC Report
Thu 2 Feb 2017
SRR4062676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188340
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA22891.21535520866518No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21991.1675692895826697No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14320.760327068068387No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA10450.5548476160135924No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC9980.5298927471593926No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7400.3929064457895296No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA6920.36742062227885736No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA6840.3631729850270787No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA6130.32547520441754274No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG6080.32282043113518105No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6030.32016565785281936No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA5940.31538706594456833No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5670.30105129021981525No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG5400.2867155144950621No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC4970.2638844642667516No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA4880.2591058723585006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.24848677922905382No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA4610.24477009663374746No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA4490.23839864075607944No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA4160.22087713709249232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.20388658808537752No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG3470.18424126579590103No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3400.18052458320059467No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC3350.17786980991823298No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT3120.16565785281936923No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA3050.16194117022406285No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2920.15503875968992248No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA2810.14919825846872678No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA2750.14601253052989274No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG2740.14548157587342042No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT2710.1438887119040034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2610.13857916533928002No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2550.135393437400446No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA2480.13167675480513963No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG2450.13008389083572264No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA2420.12849102686630562No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2310.12265052564510989No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT2290.12158861633216525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.11734097908038654No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA2170.11521716045449718No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2150.1141552511415525No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2130.11309334182860784No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2090.1109695232027185No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA2090.1109695232027185No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTA2080.11043856854624616No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTA2030.10778379526388447No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1920.10194329404268876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1910.10141233938621642No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1910.10141233938621642No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC1890.10035043007327175No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGT250.00234783835.19978739
CTAGTGT250.00234783835.1997874
ATACATG702.7284841E-1134.5804021
CCTGTGG300.005733032229.3331578
AAGCATT454.0517065E-529.33315738
ATGCGTT300.005733032229.33315740
CATCCTT300.005733032229.33315716
CGTTGCT407.010206E-427.49983243
TGTACTG508.305115E-526.3998434
TCTGCAC450.001393889124.4442963
CGCGGTA1101.9826984E-1023.99985743
GGCCGCG1101.9826984E-1023.99985740
GCGTTGC551.5867273E-423.99985742
AAGTCAA651.8029517E-523.69216520
GCCGCGG1153.45608E-1022.95638541
ACATAAA500.00256855522.005711
AGTCAAA703.1969546E-521.99986621
GCGGTAT1205.8935257E-1021.99986644
AGGGTCC602.8604423E-421.99986619
TAACGGC1205.8935257E-1021.99986636
Produced by FastQC (version 0.11.5)