##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062676_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	188340
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.295035573961982	32.0	32.0	32.0	21.0	32.0
2	31.314537538494214	32.0	32.0	32.0	32.0	32.0
3	31.45682276733567	32.0	32.0	32.0	32.0	32.0
4	31.608622703621112	32.0	32.0	32.0	32.0	32.0
5	31.517771052352128	32.0	32.0	32.0	32.0	32.0
6	35.06784538600404	36.0	36.0	36.0	36.0	36.0
7	35.05978018477222	36.0	36.0	36.0	36.0	36.0
8	35.03786237655304	36.0	36.0	36.0	36.0	36.0
9	34.96109164277371	36.0	36.0	36.0	36.0	36.0
10	34.98728363597749	36.0	36.0	36.0	36.0	36.0
11	35.02750876075183	36.0	36.0	36.0	36.0	36.0
12	34.95103005203356	36.0	36.0	36.0	36.0	36.0
13	34.918742699373475	36.0	36.0	36.0	36.0	36.0
14	34.87337793352448	36.0	36.0	36.0	32.0	36.0
15	34.94410109376659	36.0	36.0	36.0	36.0	36.0
16	34.90995009026229	36.0	36.0	36.0	32.0	36.0
17	34.900769884251886	36.0	36.0	36.0	32.0	36.0
18	34.887368588722524	36.0	36.0	36.0	32.0	36.0
19	34.75233089094191	36.0	36.0	36.0	32.0	36.0
20	34.586996920462994	36.0	36.0	36.0	32.0	36.0
21	34.45202824678773	36.0	36.0	36.0	32.0	36.0
22	34.48311033237761	36.0	36.0	36.0	32.0	36.0
23	34.456578528193695	36.0	36.0	36.0	32.0	36.0
24	34.443989593288734	36.0	36.0	36.0	32.0	36.0
25	34.439789741956034	36.0	36.0	36.0	32.0	36.0
26	34.40138579165339	36.0	36.0	36.0	32.0	36.0
27	34.355118402888394	36.0	36.0	36.0	32.0	36.0
28	34.291419772751404	36.0	36.0	36.0	32.0	36.0
29	34.19832218328555	36.0	36.0	36.0	32.0	36.0
30	34.14426038016354	36.0	36.0	36.0	32.0	36.0
31	34.09676648614208	36.0	36.0	36.0	32.0	36.0
32	33.9964638419879	36.0	36.0	36.0	32.0	36.0
33	33.825140702983965	36.0	36.0	36.0	32.0	36.0
34	33.702001699054904	36.0	36.0	36.0	27.0	36.0
35	33.56079961771265	36.0	36.0	36.0	21.0	36.0
36	33.51780290963152	36.0	36.0	36.0	21.0	36.0
37	33.3728629075077	36.0	36.0	36.0	21.0	36.0
38	33.253015822448766	36.0	36.0	36.0	14.0	36.0
39	32.934724434533294	36.0	36.0	36.0	14.0	36.0
40	32.74288520760327	36.0	36.0	36.0	14.0	36.0
41	32.72111075714134	36.0	36.0	36.0	14.0	36.0
42	32.34823723054051	36.0	36.0	36.0	14.0	36.0
43	32.36382605925454	36.0	36.0	36.0	14.0	36.0
44	32.30056281193586	36.0	36.0	36.0	14.0	36.0
45	31.807964319847084	36.0	36.0	36.0	14.0	36.0
46	31.96343315280875	36.0	36.0	36.0	14.0	36.0
47	31.65342996708081	36.0	36.0	36.0	14.0	36.0
48	31.580121057661675	36.0	36.0	36.0	14.0	36.0
49	31.596097483274928	36.0	36.0	36.0	14.0	36.0
50	30.81852500796432	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	14.0
22	38.0
23	130.0
24	391.0
25	859.0
26	1905.0
27	3656.0
28	5780.0
29	7754.0
30	9960.0
31	12694.0
32	16656.0
33	18067.0
34	27941.0
35	82490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.0817843866171	19.84705257567711	14.249070631970259	19.82209240573553
2	11.677905803642542	25.030531513832106	38.77342961822333	24.518133064302024
3	19.283313599413823	32.691051773663446	28.934527633681817	19.09110699324091
4	10.884039503026441	19.066581713921632	35.527768928533504	34.521609854518424
5	9.895401932674948	42.14134012955294	33.123075289370284	14.840182648401825
6	27.65371137304874	35.77625570776256	20.758734204098968	15.81129871508973
7	24.38249973452267	36.08686418179887	22.243283423595624	17.287352660082828
8	30.114686205798023	29.418604651162788	22.649994690453433	17.81671445258575
9	28.863757035149195	16.56100669002867	20.5277689285335	34.04746734628863
10	17.081873208028036	28.96198364659658	32.02134437719019	21.934798768185196
11	30.75395561219072	26.56313050865456	20.743867473717746	21.939046405436976
12	22.872464691515347	30.193267494955933	28.8828714027822	18.051396410746523
13	31.07624508866943	23.160242115323353	27.032494424976107	18.731018371031112
14	25.448656684719122	21.954444090474674	28.082722735478388	24.51417648932781
15	25.71838165020707	25.294148879685675	32.122756716576404	16.864712753530846
16	22.17372836359775	28.843049803546776	28.92003822873527	20.06318360412021
17	19.123924816820644	28.17298502707869	31.116066687904855	21.587023468195817
18	22.120101943294042	24.374004460019112	35.001592863969414	18.504300732717425
19	20.25698205373261	32.94042688754381	30.820855898906235	15.981735159817351
20	21.6448975257513	23.469257725390253	36.72507167887862	18.160773069979825
21	23.041308272273547	27.733885526176067	27.46469151534459	21.760114686205796
22	20.289901242433896	29.53222894764787	28.592970160348308	21.584899649569927
23	19.17064882659021	28.64075607943082	29.27790166719762	22.910693426781354
24	17.7715833067856	33.49633641287034	29.36126154826378	19.37081873208028
25	22.948391207390888	27.411065095040883	28.01263672082404	21.627906976744185
26	17.587872995646173	27.48539874694701	31.697462036742063	23.229266220664755
27	21.833917383455454	31.209514707443986	30.525114155251142	16.43145375384942
28	19.630986513751726	26.2419029414888	32.09939471169162	22.027715833067855
29	17.481682064351705	31.12243814378252	34.04587448231921	17.350005309546564
30	22.346288626951257	28.00520335563343	32.06594456833386	17.582563449081448
31	16.48720399277902	30.227779547626632	31.193055113093344	22.09196134650101
32	16.259955399808856	31.67463098651375	31.922586811086333	20.14282680259106
33	17.606987363279174	30.57449293830307	30.757672294786026	21.06084740363173
34	15.97483274928321	32.12753530848465	33.48624827439737	18.411383667834766
35	20.24105341403844	31.19464797706276	30.160879260911116	18.403419347987683
36	19.08888180949347	32.03037060635022	30.26335350961028	18.617394074546034
37	20.335032388234044	32.29797175321227	30.441754274184984	16.925241584368695
38	18.771901879579485	32.30859084634172	28.245725815015398	20.673781459063395
39	16.69055962620792	32.74875225655729	30.779972390357862	19.780715726876924
40	18.879154720186897	32.76892853350324	28.72517786980992	19.626738876499946
41	16.43145375384942	32.62026122969098	30.067431241371988	20.88085377508761
42	20.34246575342466	32.7312307528937	29.840182648401825	17.08612084527981
43	19.11118190506531	30.40405649357545	27.788573855792713	22.69618774556653
44	18.399171710735903	31.04067112668578	32.862376553042374	17.697780609535947
45	20.139110119995753	31.126685781034304	29.58426250398216	19.149941594987787
46	18.00520335563343	31.736221726664542	31.210045662100455	19.048529255601572
47	19.88849952214081	30.71572687692471	31.144738239354357	18.25103536158012
48	17.450886694276306	37.847509822661145	26.034299670808114	18.66730381225443
49	16.13836678347669	32.07656366146331	33.19156844005522	18.59350111500478
50	15.606881172347881	37.41212700435383	25.36529680365297	21.615695019645322
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57.0
1	47.0
2	37.0
3	155.5
4	274.0
5	298.0
6	322.0
7	268.0
8	214.0
9	292.5
10	371.0
11	585.5
12	800.0
13	1342.0
14	1884.0
15	2403.0
16	2922.0
17	3391.0
18	3860.0
19	4148.0
20	4436.0
21	5065.5
22	5695.0
23	5866.0
24	6037.0
25	5192.0
26	4347.0
27	4597.5
28	4848.0
29	5286.0
30	5724.0
31	5959.5
32	6195.0
33	6877.0
34	7559.0
35	7704.5
36	7850.0
37	8353.5
38	8857.0
39	8830.0
40	8803.0
41	9660.5
42	10518.0
43	11663.0
44	12808.0
45	15368.0
46	17928.0
47	20143.5
48	22359.0
49	18930.5
50	15502.0
51	11670.5
52	7839.0
53	5942.0
54	4045.0
55	3627.5
56	3210.0
57	3122.0
58	3034.0
59	2641.0
60	2248.0
61	2094.5
62	1941.0
63	1748.5
64	1556.0
65	1428.0
66	1300.0
67	1133.5
68	967.0
69	850.5
70	734.0
71	617.5
72	501.0
73	417.5
74	334.0
75	272.0
76	210.0
77	161.5
78	113.0
79	80.0
80	47.0
81	41.5
82	36.0
83	21.5
84	7.0
85	6.0
86	5.0
87	3.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.021238186258893493
2	0.005309546564723373
3	5.309546564723373E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	188340.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.75130083890836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.03588339058582	37.0749707974939
2	10.119766038436543	9.259849208877561
3	3.1206480363940208	4.283211213762344
4	1.430925633645901	2.6186683657215672
5	0.8007613035001393	1.8317935648295636
6	0.5558908179370532	1.5259636827014973
7	0.4038622226348529	1.2934055431666136
8	0.2936124779500511	1.0746522247000105
9	0.22862315476743106	0.9413826059254539
>10	1.6444619812459382	14.513645534671337
>50	0.20193111131742644	6.464372942550706
>100	0.14738650078915608	11.894977168949772
>500	0.011605236282610714	3.525007964319847
>1k	0.004642094513044285	3.698099182329829
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2289	1.21535520866518	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2199	1.1675692895826697	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1432	0.760327068068387	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1045	0.5548476160135924	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	998	0.5298927471593926	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	740	0.3929064457895296	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	692	0.36742062227885736	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	684	0.3631729850270787	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	613	0.32547520441754274	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	608	0.32282043113518105	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	603	0.32016565785281936	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	594	0.31538706594456833	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	567	0.30105129021981525	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	540	0.2867155144950621	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	497	0.2638844642667516	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	488	0.2591058723585006	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	468	0.24848677922905382	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA	461	0.24477009663374746	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	449	0.23839864075607944	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	416	0.22087713709249232	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	384	0.20388658808537752	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	347	0.18424126579590103	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	340	0.18052458320059467	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	335	0.17786980991823298	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	312	0.16565785281936923	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	305	0.16194117022406285	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	292	0.15503875968992248	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	281	0.14919825846872678	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	275	0.14601253052989274	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	274	0.14548157587342042	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	271	0.1438887119040034	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	261	0.13857916533928002	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	255	0.135393437400446	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA	248	0.13167675480513963	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	245	0.13008389083572264	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	242	0.12849102686630562	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	231	0.12265052564510989	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	229	0.12158861633216525	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	221	0.11734097908038654	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	217	0.11521716045449718	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	215	0.1141552511415525	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	213	0.11309334182860784	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	209	0.1109695232027185	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	209	0.1109695232027185	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTA	208	0.11043856854624616	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTA	203	0.10778379526388447	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	192	0.10194329404268876	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	191	0.10141233938621642	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	191	0.10141233938621642	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	189	0.10035043007327175	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.309546564723373E-4	0.0
11	0.0	0.0	0.0	5.309546564723373E-4	0.0
12	0.0	0.0	0.0	5.309546564723373E-4	0.0
13	0.0	0.0	0.0	5.309546564723373E-4	0.0
14	0.0	0.0	0.0	5.309546564723373E-4	0.0
15	0.0	0.0	0.0	5.309546564723373E-4	0.0
16	0.0	0.0	0.0	5.309546564723373E-4	0.0
17	0.0	0.0	0.0	5.309546564723373E-4	0.0
18	0.0	0.0	0.0	0.0010619093129446745	0.0
19	0.0	0.0	0.0	0.0015928639694170119	0.0
20	0.0	0.0	0.0	0.004247637251778698	0.0
21	0.0	0.0	0.0	0.0063714558776680474	0.0
22	0.0	0.0	0.0	0.01752150366358713	0.0
23	0.0	0.0	0.0	0.03451205267070192	0.0
24	0.0	0.0	0.0	0.06477646808962514	0.0
25	0.0	0.0	0.0	0.07858128915790591	0.0
26	0.0	0.0	0.0	0.11150047785919083	0.0
27	0.0	0.0	0.0	0.1481363491557821	0.0
28	0.0	0.0	0.0	0.21185090793246256	0.0
29	0.0	0.0	0.0	0.3068917914410109	0.0
30	0.0	0.0	0.0	0.46617818838271213	0.0
31	0.0	0.0	0.0	0.7109482850164596	0.0
32	0.0	0.0	0.0	1.0496973558458107	0.0
33	0.0	0.0	0.0	1.3077413188913667	0.0
34	0.0	0.0	0.0	1.6401189338430497	0.0
35	0.0	0.0	0.0	2.007539556121907	0.0
36	0.0	0.0	0.0	2.4657534246575343	0.0
37	0.0	0.0	0.0	3.142189657003292	0.0
38	0.0	0.0	0.0	3.787299564617182	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGT	25	0.002347838	35.199787	39
CTAGTGT	25	0.002347838	35.199787	4
ATACATG	70	2.7284841E-11	34.580402	1
CCTGTGG	30	0.0057330322	29.333157	8
AAGCATT	45	4.0517065E-5	29.333157	38
ATGCGTT	30	0.0057330322	29.333157	40
CATCCTT	30	0.0057330322	29.333157	16
CGTTGCT	40	7.010206E-4	27.499832	43
TGTACTG	50	8.305115E-5	26.39984	34
TCTGCAC	45	0.0013938891	24.444296	3
CGCGGTA	110	1.9826984E-10	23.999857	43
GGCCGCG	110	1.9826984E-10	23.999857	40
GCGTTGC	55	1.5867273E-4	23.999857	42
AAGTCAA	65	1.8029517E-5	23.692165	20
GCCGCGG	115	3.45608E-10	22.956385	41
ACATAAA	50	0.002568555	22.00571	1
AGTCAAA	70	3.1969546E-5	21.999866	21
GCGGTAT	120	5.8935257E-10	21.999866	44
AGGGTCC	60	2.8604423E-4	21.999866	19
TAACGGC	120	5.8935257E-10	21.999866	36
>>END_MODULE