FastQCFastQC Report
Thu 2 Feb 2017
SRR4062675_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062675_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455102
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35440.24355680907592733No Hit
GTACATGGAAGCAGTGGTATCAACG26640.18307994903450067No Hit
TCCATGTACTCTGCGTTGATACCAC24350.16734222068281124No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20930.1438387137121659No Hit
TATCAACGCAGAGTACTTTTTTTTT20510.1409523181192796No Hit
GCGCAAGACGGACCAGAGCGAAAGC17950.12335904974359185No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17540.12054137785529812No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15970.10975175623427086No Hit
GAATAGGACCGCGGTTCTATTTTGT15360.10555961025412652No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15040.10336045170716555No Hit
GATTAAGAGGGACGGCCGGGGGCAT14860.1021234250245No Hit
GTATCTGATCGTCTTCGAACCTCCG14720.10116129316020459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1550.012.85950851
AACGCCA1650.012.669764515
CAAGACG3100.012.5619684
CGACCAT1900.012.50047510
GTCGTAG2400.012.271311
ACAGCGT701.09041925E-412.214758
GCATTAT701.0999151E-412.203411
ATACCGT2500.012.1596266
TTTACGA550.00306740612.0913710
GTTTACG550.00306740612.091379
TAATACG550.003072945212.0884574
ATACGCG550.003072945212.0884574
ACCGTCG2450.012.0208658
AACCGCG1750.011.9433117
AAGACGG3500.011.9408455
TCGCGTA1459.640644E-1111.7935519
CGTCGTA2500.011.78044810
CCGACCA2350.011.723859
CCGTCGT2550.011.54945859
GCTCGTA1001.9282215E-611.4004349