##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062675_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1455102
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.141303496249748	32.0	32.0	32.0	32.0	32.0
2	30.922250811283334	32.0	32.0	32.0	32.0	32.0
3	30.915063686257046	32.0	32.0	32.0	32.0	32.0
4	30.92214635125235	32.0	32.0	32.0	32.0	32.0
5	30.841605605655136	32.0	32.0	32.0	32.0	32.0
6	34.4088723677103	36.0	36.0	36.0	32.0	36.0
7	34.310515001697475	36.0	36.0	36.0	32.0	36.0
8	34.316764048156074	36.0	36.0	36.0	32.0	36.0
9	34.39274291424244	36.0	36.0	36.0	32.0	36.0
10	34.17731540469328	36.0	36.0	36.0	32.0	36.0
11	34.39040287210106	36.0	36.0	36.0	32.0	36.0
12	34.233132797563336	36.0	36.0	36.0	32.0	36.0
13	34.329732211212686	36.0	36.0	36.0	32.0	36.0
14	34.25615042794251	36.0	36.0	36.0	32.0	36.0
15	34.21987805665857	36.0	36.0	36.0	32.0	36.0
16	34.21458083350858	36.0	36.0	36.0	32.0	36.0
17	34.14571830703277	36.0	36.0	36.0	32.0	36.0
18	34.167923623223665	36.0	36.0	36.0	32.0	36.0
19	34.147243973274726	36.0	36.0	36.0	32.0	36.0
20	34.11766872700333	36.0	36.0	36.0	32.0	36.0
21	34.08089054925359	36.0	36.0	36.0	32.0	36.0
22	34.04858628467283	36.0	36.0	36.0	32.0	36.0
23	34.02485324052884	36.0	36.0	36.0	32.0	36.0
24	33.9924101540648	36.0	36.0	36.0	32.0	36.0
25	33.49160127606174	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	54.0
5	132.0
6	246.0
7	62.0
8	187.0
9	271.0
10	190.0
11	49.0
12	113.0
13	97.0
14	305.0
15	411.0
16	642.0
17	925.0
18	1384.0
19	1738.0
20	2478.0
21	3048.0
22	4233.0
23	5925.0
24	7786.0
25	10561.0
26	14950.0
27	19257.0
28	26514.0
29	35517.0
30	47587.0
31	66674.0
32	99034.0
33	147552.0
34	344871.0
35	612301.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31661090385244	17.909623003054353	10.990452049936355	25.783314043156846
2	16.491472108499437	20.519996233803813	37.20740676087197	25.78112489682478
3	19.594907582407462	24.642927342879094	27.965892018424416	27.796273056289028
4	12.36026415779084	15.466688844777055	36.37434634143659	35.79870065599551
5	14.228192533368164	37.013759749210266	33.725856847735294	15.032190869686271
6	33.42706826469828	35.69272635267069	17.39158833268246	13.488617049948573
7	29.37726680686427	30.696398828721893	21.442394050633435	18.483940313780398
8	27.79993768171866	32.806241733026006	20.13128109465569	19.26253949059964
9	27.236281013168494	14.333179472751198	19.14215961097808	39.288379903102225
10	15.65089834243728	27.036966448993017	31.971949891254916	25.34018531731479
11	37.19343996091774	21.595698199670416	22.21956933831953	18.991292501092307
12	24.13802832603081	24.289079386794626	29.22056964486141	22.35232264231315
13	29.209329976461067	19.884789281494932	25.818444036035686	25.087436706008308
14	23.169144047748475	20.165872831288077	25.521337221544066	31.143645899419383
15	24.93850808046824	27.44497032219358	23.016520083690935	24.60000151364725
16	25.287262214449214	26.18504908621685	24.464468651764783	24.06322004756915
17	23.41426627087832	26.15705476218983	25.799248882888758	24.629430084043097
18	24.294151932897552	25.20277928214035	27.08994956581458	23.413119219147514
19	24.820804809367697	25.60750197500076	25.83521199258996	23.736481223041583
20	25.01561945234209	24.71836820072166	25.920515565781976	24.34549678115427
21	26.236584741493925	24.857761788499282	24.75639899917148	24.14925447083532
22	25.080494294850702	25.10258806881144	25.530973268598345	24.28594436773951
23	23.762739415678126	24.843073522317248	26.041915421772817	25.35227164023181
24	23.998706232107462	25.72588636864127	25.83386093371504	24.441546465536227
25	24.172375921761414	25.258602418393668	25.81434300672449	24.754678653120433
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	174.0
1	174.0
2	524.5
3	875.0
4	875.0
5	875.0
6	2275.5
7	3676.0
8	3676.0
9	3676.0
10	4066.5
11	4457.0
12	4457.0
13	4457.0
14	4934.0
15	5411.0
16	5411.0
17	5411.0
18	8609.5
19	11808.0
20	11808.0
21	11808.0
22	18696.0
23	25584.0
24	25584.0
25	25584.0
26	37068.0
27	48552.0
28	48552.0
29	48552.0
30	60166.0
31	71780.0
32	71780.0
33	71780.0
34	85991.5
35	100203.0
36	100203.0
37	100203.0
38	111817.0
39	123431.0
40	123431.0
41	123431.0
42	139189.5
43	154948.0
44	154948.0
45	154948.0
46	174666.5
47	194385.0
48	194385.0
49	194385.0
50	191417.0
51	188449.0
52	188449.0
53	188449.0
54	171718.5
55	154988.0
56	154988.0
57	154988.0
58	141144.5
59	127301.0
60	127301.0
61	127301.0
62	112906.5
63	98512.0
64	98512.0
65	98512.0
66	81928.0
67	65344.0
68	65344.0
69	65344.0
70	49722.5
71	34101.0
72	34101.0
73	34101.0
74	27000.0
75	19899.0
76	19899.0
77	19899.0
78	16160.0
79	12421.0
80	12421.0
81	12421.0
82	8719.5
83	5018.0
84	5018.0
85	5018.0
86	3650.0
87	2282.0
88	2282.0
89	2282.0
90	1504.5
91	727.0
92	727.0
93	727.0
94	444.0
95	161.0
96	161.0
97	161.0
98	388.0
99	615.0
100	615.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008934081597028937
2	0.003642356343404105
3	0.005223001549032301
4	0.01470687278280148
5	0.03415568118248755
6	0.044395513166774564
7	0.0662496512271992
8	0.0876227233554761
9	0.09655680495250504
10	0.11703646892107908
11	0.1207475489690757
12	0.1340799476600266
13	0.12129733860581594
14	0.1250771423584051
15	0.11401262591900774
16	0.11263815182715714
17	0.12370266826655452
18	0.13201823652225067
19	0.1331865395003237
20	0.1227405364022591
21	0.1309873809533627
22	0.15146704492193674
23	0.13971529143661404
24	0.136210382502395
25	0.1309873809533627
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1455102.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.70654457105928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.29891483296791	42.83463074175333
2	13.855242363633389	15.159448678178775
3	4.022920525467068	6.602402430968802
4	1.5119140459858136	3.308463725773339
5	0.6877714524672793	1.88127998095517
6	0.3827160234110052	1.2562242715675633
7	0.21896888800094377	0.8385321861769244
8	0.14593339230223312	0.6386809304310398
9	0.1127118490935748	0.5549468216511743
>10	0.580116699687131	6.047807530777397
>50	0.07909659363133434	3.058525135291292
>100	0.08949053840150085	10.720088666927255
>500	0.010180764522381829	3.755316936245672
>1k	0.0040220304285952906	3.3436519633022894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3544	0.24355680907592733	No Hit
GTACATGGAAGCAGTGGTATCAACG	2664	0.18307994903450067	No Hit
TCCATGTACTCTGCGTTGATACCAC	2435	0.16734222068281124	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2093	0.1438387137121659	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2051	0.1409523181192796	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1795	0.12335904974359185	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1754	0.12054137785529812	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1597	0.10975175623427086	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1536	0.10555961025412652	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1504	0.10336045170716555	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1486	0.1021234250245	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1472	0.10116129316020459	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.872370459253029E-5
2	0.0	0.0	0.0	0.0	6.872370459253029E-5
3	0.0	0.0	0.0	0.0	6.872370459253029E-5
4	0.0	0.0	0.0	6.872370459253029E-5	6.872370459253029E-5
5	0.0	6.872370459253029E-5	0.0	6.872370459253029E-5	6.872370459253029E-5
6	0.0	6.872370459253029E-5	0.0	6.872370459253029E-5	6.872370459253029E-5
7	0.0	6.872370459253029E-5	0.0	6.872370459253029E-5	6.872370459253029E-5
8	0.0	6.872370459253029E-5	0.0	1.3744740918506058E-4	6.872370459253029E-5
9	0.0	6.872370459253029E-5	0.0	1.3744740918506058E-4	6.872370459253029E-5
10	6.872370459253029E-5	6.872370459253029E-5	0.0	1.3744740918506058E-4	6.872370459253029E-5
11	6.872370459253029E-5	6.872370459253029E-5	0.0	1.3744740918506058E-4	6.872370459253029E-5
12	6.872370459253029E-5	1.3744740918506058E-4	0.0	1.3744740918506058E-4	2.0617111377759084E-4
13	6.872370459253029E-5	1.3744740918506058E-4	0.0	1.3744740918506058E-4	2.0617111377759084E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	155	0.0	12.8595085	1
AACGCCA	165	0.0	12.6697645	15
CAAGACG	310	0.0	12.561968	4
CGACCAT	190	0.0	12.500475	10
GTCGTAG	240	0.0	12.2713	11
ACAGCGT	70	1.09041925E-4	12.21475	8
GCATTAT	70	1.0999151E-4	12.20341	1
ATACCGT	250	0.0	12.159626	6
TTTACGA	55	0.003067406	12.09137	10
GTTTACG	55	0.003067406	12.09137	9
TAATACG	55	0.0030729452	12.088457	4
ATACGCG	55	0.0030729452	12.088457	4
ACCGTCG	245	0.0	12.020865	8
AACCGCG	175	0.0	11.943311	7
AAGACGG	350	0.0	11.940845	5
TCGCGTA	145	9.640644E-11	11.793551	9
CGTCGTA	250	0.0	11.780448	10
CCGACCA	235	0.0	11.72385	9
CCGTCGT	255	0.0	11.5494585	9
GCTCGTA	100	1.9282215E-6	11.400434	9
>>END_MODULE