FastQCFastQC Report
Thu 2 Feb 2017
SRR4062675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455102
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17030.11703646892107908No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC16730.11497475778330317No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15790.10851472955160531No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15570.10700280805056965No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15450.10617812359545928No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15090.10370407023012819No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14860.1021234250245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG4200.020.9522344
GTATAGT856.102495E-620.7107181
CGTCTAT654.9250666E-420.3124351
GTATTAA2350.017.7913881
GTCCTAA3350.017.7354851
AGGACGT4900.017.510085
ATACCGT3400.017.4704676
GACGTGA2650.017.433847
TTAGGAC3700.017.2437153
TACCGTC3600.017.1109927
ATAACGC5950.017.0088673
CTATACT1702.4683686E-916.8234124
TGTAGGA6650.016.5418072
TATGACG800.001988706216.4998844
TTAACGG2151.2732926E-1116.37197935
CGTCGTA3650.016.27385910
CTATTAG953.3409492E-416.2143141
TGGTCGG5850.016.17082637
TAACGGC2201.8189894E-1115.99988736
GTCTAGA2500.015.84369851
Produced by FastQC (version 0.11.5)