##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062675_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1455102
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2184059949062	32.0	32.0	32.0	32.0	32.0
2	31.29332582870479	32.0	32.0	32.0	32.0	32.0
3	31.42455168091309	32.0	32.0	32.0	32.0	32.0
4	31.55509579397183	32.0	32.0	32.0	32.0	32.0
5	31.46100960619943	32.0	32.0	32.0	32.0	32.0
6	35.04194413862396	36.0	36.0	36.0	36.0	36.0
7	35.05351927218848	36.0	36.0	36.0	36.0	36.0
8	34.995734319655945	36.0	36.0	36.0	36.0	36.0
9	35.13247250020961	36.0	36.0	36.0	36.0	36.0
10	34.99342726489277	36.0	36.0	36.0	36.0	36.0
11	35.14531696059795	36.0	36.0	36.0	36.0	36.0
12	35.03852857050571	36.0	36.0	36.0	36.0	36.0
13	35.09510329860037	36.0	36.0	36.0	36.0	36.0
14	35.050645246862416	36.0	36.0	36.0	36.0	36.0
15	35.00768331017345	36.0	36.0	36.0	36.0	36.0
16	35.01841176769739	36.0	36.0	36.0	36.0	36.0
17	34.995418190614814	36.0	36.0	36.0	36.0	36.0
18	35.011233576752694	36.0	36.0	36.0	36.0	36.0
19	34.99335373052886	36.0	36.0	36.0	36.0	36.0
20	34.97537973282973	36.0	36.0	36.0	36.0	36.0
21	34.96674734829586	36.0	36.0	36.0	32.0	36.0
22	34.958683996036015	36.0	36.0	36.0	32.0	36.0
23	34.914606673621506	36.0	36.0	36.0	32.0	36.0
24	34.8937105440031	36.0	36.0	36.0	32.0	36.0
25	34.86267079558684	36.0	36.0	36.0	32.0	36.0
26	34.81733514214124	36.0	36.0	36.0	32.0	36.0
27	34.80609881644036	36.0	36.0	36.0	32.0	36.0
28	34.781252448281975	36.0	36.0	36.0	32.0	36.0
29	34.7567084644238	36.0	36.0	36.0	32.0	36.0
30	34.74122913720138	36.0	36.0	36.0	32.0	36.0
31	34.74522473338639	36.0	36.0	36.0	32.0	36.0
32	34.6971717446612	36.0	36.0	36.0	32.0	36.0
33	34.67574575528039	36.0	36.0	36.0	32.0	36.0
34	34.65238038295597	36.0	36.0	36.0	32.0	36.0
35	34.628375880178844	36.0	36.0	36.0	32.0	36.0
36	34.59991808134413	36.0	36.0	36.0	32.0	36.0
37	34.59002530406803	36.0	36.0	36.0	32.0	36.0
38	34.545468290195465	36.0	36.0	36.0	32.0	36.0
39	34.51731287566095	36.0	36.0	36.0	32.0	36.0
40	34.497358260795465	36.0	36.0	36.0	32.0	36.0
41	34.46321288816866	36.0	36.0	36.0	32.0	36.0
42	34.43480388316421	36.0	36.0	36.0	32.0	36.0
43	34.42633437381022	36.0	36.0	36.0	32.0	36.0
44	34.39386379786434	36.0	36.0	36.0	32.0	36.0
45	34.32818936404458	36.0	36.0	36.0	32.0	36.0
46	34.34445282873641	36.0	36.0	36.0	32.0	36.0
47	34.29665068153298	36.0	36.0	36.0	32.0	36.0
48	34.2597103158404	36.0	36.0	36.0	32.0	36.0
49	34.24213903905019	36.0	36.0	36.0	32.0	36.0
50	33.68500764894832	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	28.0
22	117.0
23	406.0
24	1086.0
25	2375.0
26	4912.0
27	9076.0
28	15487.0
29	23851.0
30	35803.0
31	51965.0
32	78083.0
33	129005.0
34	276457.0
35	826445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.12672402771916	18.307858115938245	11.507425364875955	26.057992491466635
2	15.999197277340679	21.120952710838406	36.95919309878437	25.920656913036545
3	18.82022742173358	25.02628696152963	28.482146170625406	27.671339446111386
4	12.190502370971066	16.057453096007144	36.683801800563536	35.06824273245825
5	14.162237423905676	37.117810297834794	33.52033053352961	15.199621744729921
6	33.55015796241202	36.09095776008819	16.995339816767725	13.363544460732063
7	29.486317797652674	30.67860534862848	21.39891224120371	18.436164612515135
8	27.7711115784323	32.96531789524033	19.921283868759716	19.342286657567648
9	27.434571597042684	14.061144854450067	18.889466168007466	39.61481738049979
10	15.695188378546659	26.925466393421218	31.963738624508796	25.415606603523326
11	37.45826753038619	21.514230617509973	22.22194732740385	18.805554524699986
12	24.30503153730804	24.174868840809786	29.09919716968295	22.420902452199226
13	29.502811486754883	19.816892561483662	25.56377491062482	25.116521041136636
14	23.218853386223095	20.10938064822947	25.427839422940796	31.243926542606637
15	24.97027699776373	27.411342984890407	22.831320416025818	24.787059601320045
16	25.357672520551823	26.1629081672625	24.381864639042487	24.097554673143186
17	23.569962792986335	26.062640282262002	25.665142374898803	24.70225454985286
18	24.609889890880503	24.93900771217413	26.993296689854045	23.457805707091325
19	25.044223703905296	25.560819791327344	25.774756683723894	23.62019982104347
20	25.15631206609571	24.682118504407253	25.849115732093008	24.31245369740403
21	26.49566834489953	24.77303996558317	24.60171177003399	24.12957991948331
22	25.285924972957226	25.02697405405257	25.454023154390555	24.233077818599657
23	23.98216757313233	24.67744529249496	25.962509844670684	25.377877289702027
24	24.143668278924775	25.60280997483338	25.663699177102362	24.589822569139482
25	24.452443883659015	25.05253927216099	25.744380806293993	24.750636037886004
26	23.821491551794992	25.921413069324352	26.260770722602267	23.996324656278393
27	24.97955469788372	25.322348536391264	25.483093281433188	24.215003484291824
28	23.962168975095903	25.195278406599677	26.131707605377493	24.71084501292693
29	23.807609363467304	25.386467752776092	26.155073664938953	24.65084921881765
30	23.986565890226252	25.648717409501188	26.354647303075662	24.010069397196897
31	24.399320460008987	25.36055891614471	25.37773984229284	24.86238078155346
32	24.032542048598653	25.553947420868088	25.394302255099642	25.019208275433613
33	23.80149295375857	25.04951542915892	25.98587590423214	25.16311571285037
34	24.40076365780543	25.476701976906085	26.24358979645413	23.878944568834353
35	25.21995021654839	25.139199863652166	25.870901146448837	23.769948773350595
36	23.789191410636505	25.97419287445141	25.666104506763098	24.570511208148982
37	25.007937587880434	25.53168094058011	25.39230926766646	24.068072203872994
38	23.889459295637007	25.335474763968435	25.92965991387545	24.845406026519104
39	24.605285402672802	25.177891309337763	25.528794544987228	24.688028743002207
40	24.954126927184486	25.258023148892654	25.845816994272568	23.942032929650292
41	23.68315073445023	25.60384083040227	26.5741508155442	24.1388576196033
42	25.190948813210344	25.84677912613686	25.77510030224685	23.18717175840594
43	24.078655654380242	24.789808549503746	26.062640282262002	25.068895513854013
44	23.63854905016968	25.442133953496043	26.03095865444484	24.888358341889436
45	23.94979870826925	25.744586977407767	25.857431300348704	24.44818301397428
46	23.628119285190515	25.246357469344055	26.061352442201606	25.06417080326383
47	24.067728585350032	25.028348528144416	26.47745656318251	24.426466323323037
48	24.602055388556956	25.90650002542777	25.314376586658533	24.177067999356748
49	23.499108653551435	25.8464355076139	25.936738455448484	24.71771738338618
50	23.518901080474084	26.248744074298568	25.436498609719454	24.795856235507888
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	17.5
2	19.0
3	90.5
4	162.0
5	186.5
6	211.0
7	209.0
8	207.0
9	283.5
10	360.0
11	574.0
12	788.0
13	1306.5
14	1825.0
15	2619.5
16	3414.0
17	4152.5
18	4891.0
19	5627.0
20	6363.0
21	7430.0
22	8497.0
23	9283.0
24	10069.0
25	11417.5
26	12766.0
27	15615.5
28	18465.0
29	21342.5
30	24220.0
31	26458.5
32	28697.0
33	32945.5
34	37194.0
35	42229.5
36	47265.0
37	54477.0
38	61689.0
39	65114.5
40	68540.0
41	73768.5
42	78997.0
43	80332.0
44	81667.0
45	89066.5
46	96466.0
47	102624.0
48	108782.0
49	110386.5
50	111991.0
51	104404.0
52	96817.0
53	93582.0
54	90347.0
55	91697.0
56	93047.0
57	90175.5
58	87304.0
59	79652.0
60	72000.0
61	63480.5
62	54961.0
63	48056.5
64	41152.0
65	35980.0
66	30808.0
67	27254.0
68	23700.0
69	21530.5
70	19361.0
71	14928.5
72	10496.0
73	9510.5
74	8525.0
75	6249.0
76	3973.0
77	3630.5
78	3288.0
79	2766.5
80	2245.0
81	1830.5
82	1416.0
83	1207.0
84	998.0
85	823.5
86	649.0
87	481.5
88	314.0
89	195.5
90	77.0
91	56.5
92	36.0
93	26.5
94	17.0
95	10.0
96	3.0
97	3.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015394109828726783
2	0.003711080047996635
3	5.497896367402422E-4
4	1.3744740918506055E-4
5	0.0
6	6.185133413327726E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	6.872370459253028E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1455102.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.44514731233657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2064128091156	46.29230464710505
2	13.506069407367693	15.787284322484943
3	3.8344945490735345	6.723227963668631
4	1.3838221815728686	3.2351076502442115
5	0.643662985824155	1.8809489012995575
6	0.3441526138954391	1.2068430130226782
7	0.20249301439534267	0.8284313839238425
8	0.1411059811567241	0.6597567884285224
9	0.09512986766608496	0.5003891216592677
>10	0.48828464881951517	5.490147598866246
>50	0.07001638766504392	2.885786318451185
>100	0.07384071240030761	9.33968354133214
>500	0.007797522574702977	3.092317256203272
>1k	0.0027173184730025527	2.0777714933103932
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1703	0.11703646892107908	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1673	0.11497475778330317	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1579	0.10851472955160531	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1557	0.10700280805056965	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1545	0.10617812359545928	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1509	0.10370407023012819	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1486	0.1021234250245	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.872370459253029E-5
2	0.0	0.0	0.0	0.0	6.872370459253029E-5
3	0.0	0.0	0.0	0.0	6.872370459253029E-5
4	0.0	0.0	0.0	0.0	6.872370459253029E-5
5	0.0	0.0	0.0	0.0	6.872370459253029E-5
6	0.0	0.0	0.0	0.0	6.872370459253029E-5
7	0.0	0.0	0.0	0.0	6.872370459253029E-5
8	0.0	0.0	0.0	6.872370459253029E-5	6.872370459253029E-5
9	0.0	0.0	0.0	6.872370459253029E-5	6.872370459253029E-5
10	0.0	0.0	0.0	6.872370459253029E-5	6.872370459253029E-5
11	0.0	0.0	0.0	6.872370459253029E-5	6.872370459253029E-5
12	0.0	0.0	0.0	2.0617111377759084E-4	6.872370459253029E-5
13	0.0	0.0	0.0	2.0617111377759084E-4	1.3744740918506058E-4
14	0.0	0.0	0.0	2.0617111377759084E-4	1.3744740918506058E-4
15	0.0	0.0	0.0	2.0617111377759084E-4	1.3744740918506058E-4
16	0.0	0.0	0.0	2.0617111377759084E-4	1.3744740918506058E-4
17	0.0	0.0	0.0	2.7489481837012116E-4	1.3744740918506058E-4
18	0.0	6.872370459253029E-5	0.0	2.7489481837012116E-4	1.3744740918506058E-4
19	0.0	6.872370459253029E-5	0.0	2.7489481837012116E-4	1.3744740918506058E-4
20	0.0	6.872370459253029E-5	0.0	4.123422275551817E-4	2.0617111377759084E-4
21	0.0	6.872370459253029E-5	0.0	4.8106593214771197E-4	2.0617111377759084E-4
22	0.0	6.872370459253029E-5	0.0	0.0010308555688879543	2.0617111377759084E-4
23	0.0	6.872370459253029E-5	0.0	0.0019929874331833784	2.7489481837012116E-4
24	0.0	6.872370459253029E-5	0.0	0.002955119297478802	2.7489481837012116E-4
25	0.0	6.872370459253029E-5	0.0	0.003642356343404105	2.7489481837012116E-4
26	0.0	6.872370459253029E-5	0.0	0.00481065932147712	3.436185229626514E-4
27	6.872370459253029E-5	6.872370459253029E-5	0.0	0.006666199345475438	3.436185229626514E-4
28	6.872370459253029E-5	6.872370459253029E-5	0.0	0.013744740918506056	4.123422275551817E-4
29	6.872370459253029E-5	6.872370459253029E-5	0.0	0.02687096849567934	4.123422275551817E-4
30	6.872370459253029E-5	6.872370459253029E-5	0.0	0.052092568081137956	4.123422275551817E-4
31	6.872370459253029E-5	6.872370459253029E-5	0.0	0.10624684730005182	4.123422275551817E-4
32	6.872370459253029E-5	6.872370459253029E-5	0.0	0.16967882663895728	4.123422275551817E-4
33	6.872370459253029E-5	6.872370459253029E-5	0.0	0.23036185779416152	4.123422275551817E-4
34	6.872370459253029E-5	6.872370459253029E-5	0.0	0.3056143143229822	4.123422275551817E-4
35	6.872370459253029E-5	6.872370459253029E-5	0.0	0.383478271626319	4.123422275551817E-4
36	6.872370459253029E-5	6.872370459253029E-5	0.0	0.5045007154137648	4.123422275551817E-4
37	6.872370459253029E-5	1.3744740918506058E-4	0.0	0.6637335389546575	4.123422275551817E-4
38	6.872370459253029E-5	1.3744740918506058E-4	0.0	0.8768457468960938	4.123422275551817E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	420	0.0	20.952234	4
GTATAGT	85	6.102495E-6	20.710718	1
CGTCTAT	65	4.9250666E-4	20.312435	1
GTATTAA	235	0.0	17.791388	1
GTCCTAA	335	0.0	17.735485	1
AGGACGT	490	0.0	17.51008	5
ATACCGT	340	0.0	17.470467	6
GACGTGA	265	0.0	17.43384	7
TTAGGAC	370	0.0	17.243715	3
TACCGTC	360	0.0	17.110992	7
ATAACGC	595	0.0	17.008867	3
CTATACT	170	2.4683686E-9	16.823412	4
TGTAGGA	665	0.0	16.541807	2
TATGACG	80	0.0019887062	16.499884	4
TTAACGG	215	1.2732926E-11	16.371979	35
CGTCGTA	365	0.0	16.273859	10
CTATTAG	95	3.3409492E-4	16.214314	1
TGGTCGG	585	0.0	16.170826	37
TAACGGC	220	1.8189894E-11	15.999887	36
GTCTAGA	250	0.0	15.8436985	1
>>END_MODULE