FastQCFastQC Report
Thu 2 Feb 2017
SRR4062674_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062674_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798939
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40370.2244100550379974No Hit
TATCAACGCAGAGTACTTTTTTTTT25010.13902639277929935No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23650.13146638101681046No Hit
GCGCAAGACGGACCAGAGCGAAAGC21400.11895900861563401No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18870.10489516320453333No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18700.10395016173422222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG456.771247E-414.7754194
CGGTTCT2750.013.12956112
CGAACGT751.4813164E-512.6646454
AAGACGG5000.012.3494025
CGGTCCA3250.012.2783810
CAAGACG5000.011.7781194
TATTAGG907.512672E-611.6040912
AACCGCG1157.066228E-811.5662677
AGAATTT3750.011.40262317
GCGCAAG4750.011.1910491
CGAGCCG3400.011.17810815
CGGACCA5250.011.0394059
GGTTCTA3200.010.98598513
GGACGTG4250.010.9536316
GTTTTCG2000.010.926615
AGGCCCG3750.010.89462710
TCTATAC1408.409188E-910.852393
CCGTCGT2550.010.80520259
TTCGTTC1158.7914486E-710.74160118
ACGCGCG1158.8009074E-710.74070413
Produced by FastQC (version 0.11.5)