##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062674_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1798939
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15783414557136	32.0	32.0	32.0	32.0	32.0
2	30.837612059108174	32.0	32.0	32.0	32.0	32.0
3	30.821041736267876	32.0	32.0	32.0	32.0	32.0
4	30.885020003457594	32.0	32.0	32.0	32.0	32.0
5	30.706348019582656	32.0	32.0	32.0	32.0	32.0
6	34.34613235912946	36.0	36.0	36.0	32.0	36.0
7	34.226237799058225	36.0	36.0	36.0	32.0	36.0
8	34.226851494130706	36.0	36.0	36.0	32.0	36.0
9	34.37188754037797	36.0	36.0	36.0	32.0	36.0
10	34.05064929939259	36.0	36.0	36.0	32.0	36.0
11	34.36092274390627	36.0	36.0	36.0	32.0	36.0
12	34.139731808582724	36.0	36.0	36.0	32.0	36.0
13	34.264628761731224	36.0	36.0	36.0	32.0	36.0
14	34.169110792528265	36.0	36.0	36.0	32.0	36.0
15	34.11371425045541	36.0	36.0	36.0	32.0	36.0
16	34.11802290127681	36.0	36.0	36.0	32.0	36.0
17	34.047957712851854	36.0	36.0	36.0	32.0	36.0
18	34.057904687151705	36.0	36.0	36.0	32.0	36.0
19	34.04513549375493	36.0	36.0	36.0	32.0	36.0
20	34.0310021629416	36.0	36.0	36.0	32.0	36.0
21	34.014758699433386	36.0	36.0	36.0	32.0	36.0
22	33.963452901960544	36.0	36.0	36.0	32.0	36.0
23	33.94134876168675	36.0	36.0	36.0	32.0	36.0
24	33.91156064769289	36.0	36.0	36.0	32.0	36.0
25	33.47714569532374	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	74.0
5	164.0
6	290.0
7	62.0
8	285.0
9	332.0
10	219.0
11	72.0
12	146.0
13	116.0
14	305.0
15	398.0
16	631.0
17	817.0
18	1093.0
19	1635.0
20	2413.0
21	3631.0
22	5307.0
23	8029.0
24	10966.0
25	15684.0
26	21551.0
27	27815.0
28	37458.0
29	49647.0
30	63706.0
31	85704.0
32	122410.0
33	177147.0
34	399093.0
35	761728.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.496977971883354	17.4643872109727	10.890253014245117	26.148381802898836
2	17.08655849541603	20.062972656258683	36.62420311349977	26.226265734825528
3	19.559089137773945	23.43880898365471	28.232103874271974	28.769998004299374
4	12.737820895937288	15.218480270198897	35.676039306711886	36.36765952715193
5	14.75594708169129	36.310632318292804	33.54883088039441	15.384589719621491
6	34.32323099750185	35.12623099972638	16.810556733321025	13.739981269450741
7	30.278741962040804	30.336926773913625	20.67201788932648	18.712313374719088
8	28.142729413780764	32.79729777097263	19.641957992156147	19.41801482309046
9	27.34383346390798	14.207448986859442	18.82406030768135	39.624657241551226
10	16.002491110531064	26.689666955886302	31.730659526522036	25.577182407060594
11	37.46665457057098	21.223638813551116	22.095392556337288	19.21431405954062
12	24.510968488903483	23.79752965471943	28.825945861698514	22.865555994678573
13	29.5419456457008	19.47293138089752	25.24803392830308	25.737089045098593
14	23.11140127657964	20.022363520189508	25.15450754615746	31.711727657073396
15	24.959053821893725	27.51861430230662	22.386025485413256	25.136306390386398
16	25.51146248050816	25.872750160553753	24.187241847931944	24.428545511006142
17	23.796772841599186	25.967933237193524	25.48265169987683	24.752642221330458
18	24.56906820322258	24.927417325885074	26.600548155041935	23.902966315850406
19	25.236833272382057	25.232435874528324	25.502124388469465	24.02860646462015
20	25.284657281426952	24.63068855393322	25.357345209776472	24.727308954863357
21	26.30532505382497	24.528321841025424	24.670983054319244	24.495370050830363
22	25.3797113520068	24.84434910562325	25.15111345437256	24.624826087997388
23	24.1425763430062	24.588635375548527	25.75619336238775	25.512594919057513
24	24.403648275919654	25.253929244377147	25.672417776021238	24.670004703681954
25	24.50503385123713	24.953730426926914	25.629753241931564	24.911482479904393
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	276.0
1	276.0
2	663.5
3	1051.0
4	1051.0
5	1051.0
6	2667.5
7	4284.0
8	4284.0
9	4284.0
10	4637.5
11	4991.0
12	4991.0
13	4991.0
14	5562.5
15	6134.0
16	6134.0
17	6134.0
18	9900.0
19	13666.0
20	13666.0
21	13666.0
22	21200.0
23	28734.0
24	28734.0
25	28734.0
26	41962.5
27	55191.0
28	55191.0
29	55191.0
30	68753.5
31	82316.0
32	82316.0
33	82316.0
34	99573.5
35	116831.0
36	116831.0
37	116831.0
38	131172.5
39	145514.0
40	145514.0
41	145514.0
42	164351.5
43	183189.0
44	183189.0
45	183189.0
46	206146.5
47	229104.0
48	229104.0
49	229104.0
50	232501.0
51	235898.0
52	235898.0
53	235898.0
54	218832.5
55	201767.0
56	201767.0
57	201767.0
58	185835.5
59	169904.0
60	169904.0
61	169904.0
62	151290.0
63	132676.0
64	132676.0
65	132676.0
66	109916.0
67	87156.0
68	87156.0
69	87156.0
70	66544.0
71	45932.0
72	45932.0
73	45932.0
74	36134.5
75	26337.0
76	26337.0
77	26337.0
78	21377.5
79	16418.0
80	16418.0
81	16418.0
82	11450.0
83	6482.0
84	6482.0
85	6482.0
86	4825.0
87	3168.0
88	3168.0
89	3168.0
90	2096.5
91	1025.0
92	1025.0
93	1025.0
94	622.5
95	220.0
96	220.0
97	220.0
98	447.5
99	675.0
100	675.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00817148330210196
2	0.003502064272329412
3	0.00400235916837647
4	0.014675316950713726
5	0.03446475950546406
6	0.04485977567888628
7	0.06776216425348497
8	0.08938602142707451
9	0.09755750472917649
10	0.11951489183346406
11	0.12318372107114248
12	0.13502403361092288
13	0.12279460281866145
14	0.1257963721949438
15	0.11495664944725752
16	0.11301105818485228
17	0.12507372401176473
18	0.1315775576603765
19	0.13463491535844183
20	0.1232393093929255
21	0.13202226423464053
22	0.15409082798249413
23	0.1411943373288366
24	0.13752550809115818
25	0.1335231489227817
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1798939.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.38870693259445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.80390943948836	42.86042735647046
2	13.241817869760041	14.40410702746342
3	3.779753529604784	6.167277209973422
4	1.474282465660127	3.2073726784260557
5	0.7390930841307404	2.0099158574347102
6	0.4295235735151675	1.401673905633428
7	0.27980276193897374	1.0652677292620534
8	0.18514595306599713	0.8055879184849923
9	0.13867450995569067	0.678809455290109
>10	0.7504120956094453	7.558927855860742
>50	0.0775661365927899	2.9916872123115508
>100	0.08493949675134152	9.71608591055696
>500	0.011591404651006295	4.202980597527367
>1k	0.003487679275524018	2.929879285304772
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4037	0.2244100550379974	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2501	0.13902639277929935	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2365	0.13146638101681046	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2140	0.11895900861563401	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1887	0.10489516320453333	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1870	0.10395016173422222	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.558832178300654E-5
7	0.0	0.0	0.0	0.0	5.558832178300654E-5
8	0.0	0.0	0.0	0.0	1.1117664356601308E-4
9	0.0	0.0	0.0	0.0	1.1117664356601308E-4
10	0.0	0.0	0.0	0.0	1.1117664356601308E-4
11	0.0	0.0	0.0	0.0	1.1117664356601308E-4
12	0.0	0.0	0.0	0.0	3.3352993069803925E-4
13	0.0	0.0	0.0	0.0	4.4470657426405233E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGACG	45	6.771247E-4	14.775419	4
CGGTTCT	275	0.0	13.129561	12
CGAACGT	75	1.4813164E-5	12.664645	4
AAGACGG	500	0.0	12.349402	5
CGGTCCA	325	0.0	12.27838	10
CAAGACG	500	0.0	11.778119	4
TATTAGG	90	7.512672E-6	11.604091	2
AACCGCG	115	7.066228E-8	11.566267	7
AGAATTT	375	0.0	11.402623	17
GCGCAAG	475	0.0	11.191049	1
CGAGCCG	340	0.0	11.178108	15
CGGACCA	525	0.0	11.039405	9
GGTTCTA	320	0.0	10.985985	13
GGACGTG	425	0.0	10.953631	6
GTTTTCG	200	0.0	10.9266	15
AGGCCCG	375	0.0	10.894627	10
TCTATAC	140	8.409188E-9	10.85239	3
CCGTCGT	255	0.0	10.8052025	9
TTCGTTC	115	8.7914486E-7	10.741601	18
ACGCGCG	115	8.8009074E-7	10.740704	13
>>END_MODULE