FastQCFastQC Report
Thu 2 Feb 2017
SRR4062674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798939
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21490.11945930351168106No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC19190.10667398950158956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA5650.019.85820636
TAGGACG6550.019.1448444
CTAGCGG6150.018.60143729
ATACGAA6200.018.45142640
TAGCGGC6100.018.39325530
TCTAGCG6400.017.87481928
CGAATGC6400.017.87481943
CAATACG6400.017.53107338
CGTCTTA1651.6607373E-917.33315815
CGGTCCA6950.017.09335310
GTCCTAA4400.017.00551
GTATCAA37500.016.9601521
AATACGA6750.016.94797739
CGAGCCG5650.016.74319515
CGTCGTA5300.016.60360510
TCCTACA8000.016.5016672
GCAATAC6950.016.14372337
GATTTCG3550.016.11251341
GACGTGA4550.015.9558837
GCGAAAG7450.015.94614718
Produced by FastQC (version 0.11.5)