##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062674_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1798939
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.287231529251407	32.0	32.0	32.0	32.0	32.0
2	31.426301836804917	32.0	32.0	32.0	32.0	32.0
3	31.4980296719344	32.0	32.0	32.0	32.0	32.0
4	31.593945653521324	32.0	32.0	32.0	32.0	32.0
5	31.535979819215658	32.0	32.0	32.0	32.0	32.0
6	35.12452673492542	36.0	36.0	36.0	36.0	36.0
7	35.13440978265522	36.0	36.0	36.0	36.0	36.0
8	35.07788146235087	36.0	36.0	36.0	36.0	36.0
9	35.19689439163863	36.0	36.0	36.0	36.0	36.0
10	35.08690567050912	36.0	36.0	36.0	36.0	36.0
11	35.208135462069585	36.0	36.0	36.0	36.0	36.0
12	35.1193976004745	36.0	36.0	36.0	36.0	36.0
13	35.1602577964011	36.0	36.0	36.0	36.0	36.0
14	35.12760299265289	36.0	36.0	36.0	36.0	36.0
15	35.084517596205316	36.0	36.0	36.0	36.0	36.0
16	35.10053370347744	36.0	36.0	36.0	36.0	36.0
17	35.073020819494154	36.0	36.0	36.0	36.0	36.0
18	35.08729701229447	36.0	36.0	36.0	36.0	36.0
19	35.06589106134227	36.0	36.0	36.0	36.0	36.0
20	35.062022114146174	36.0	36.0	36.0	36.0	36.0
21	35.054097442992784	36.0	36.0	36.0	36.0	36.0
22	35.038014629734526	36.0	36.0	36.0	36.0	36.0
23	35.00664836328525	36.0	36.0	36.0	36.0	36.0
24	34.982457993294936	36.0	36.0	36.0	36.0	36.0
25	34.95580506064964	36.0	36.0	36.0	32.0	36.0
26	34.917758745571696	36.0	36.0	36.0	32.0	36.0
27	34.900677010170995	36.0	36.0	36.0	32.0	36.0
28	34.88532796276027	36.0	36.0	36.0	32.0	36.0
29	34.855768872652156	36.0	36.0	36.0	32.0	36.0
30	34.842925746787415	36.0	36.0	36.0	32.0	36.0
31	34.84440217261397	36.0	36.0	36.0	32.0	36.0
32	34.80842374310635	36.0	36.0	36.0	32.0	36.0
33	34.789760519950924	36.0	36.0	36.0	32.0	36.0
34	34.77385892462168	36.0	36.0	36.0	32.0	36.0
35	34.752581938576014	36.0	36.0	36.0	32.0	36.0
36	34.72330245772647	36.0	36.0	36.0	32.0	36.0
37	34.711465480486	36.0	36.0	36.0	32.0	36.0
38	34.67977680177038	36.0	36.0	36.0	32.0	36.0
39	34.66544335299863	36.0	36.0	36.0	32.0	36.0
40	34.6595626644372	36.0	36.0	36.0	32.0	36.0
41	34.6321114834911	36.0	36.0	36.0	32.0	36.0
42	34.60649916422958	36.0	36.0	36.0	32.0	36.0
43	34.595526029509614	36.0	36.0	36.0	32.0	36.0
44	34.56019798336686	36.0	36.0	36.0	32.0	36.0
45	34.53237880773056	36.0	36.0	36.0	32.0	36.0
46	34.522099415266446	36.0	36.0	36.0	32.0	36.0
47	34.490507460230724	36.0	36.0	36.0	32.0	36.0
48	34.44658379189067	36.0	36.0	36.0	32.0	36.0
49	34.43310695915759	36.0	36.0	36.0	32.0	36.0
50	33.91684487356158	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	5.0
20	8.0
21	42.0
22	142.0
23	467.0
24	1218.0
25	2774.0
26	5671.0
27	10484.0
28	18053.0
29	27850.0
30	40513.0
31	57704.0
32	84415.0
33	138333.0
34	303238.0
35	1108021.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87276909194725	18.152882354609094	11.342389379596153	26.631959173847513
2	16.454013704219665	20.55810840409103	36.32296418513483	26.664913706554476
3	18.83473082918087	23.803123186423942	28.643272786297647	28.71887319809754
4	12.49078621005551	15.631257177862	35.92605398530463	35.95190262677786
5	14.392594746125356	36.59901753200081	33.45016145628062	15.55822626559322
6	34.48592909654377	35.3925260919716	16.50963770029323	13.611907111191407
7	30.237212045544627	30.42921410898313	20.689417484417202	18.644156361055046
8	28.0518839448608	32.99841350841441	19.529188888110653	19.420513658614137
9	27.47130391858757	13.991302651173832	18.704636455154954	39.83275697508365
10	15.87941558885543	26.81235995217181	31.73181525332432	25.576409205648442
11	37.692995704690375	21.086373690269653	22.071676693873442	19.148953911166526
12	24.62923979078779	23.77734875946322	28.677737266244158	22.915674183504834
13	29.684497362056188	19.446017902774916	25.219421003157972	25.650063732010924
14	23.225857019054008	19.896672427469746	25.201743916830978	31.67572663664527
15	25.043817494645452	27.491816009325497	22.362514793442134	25.101851702586913
16	25.5301041336032	25.854739932815956	24.181253505538542	24.433902428042305
17	23.870459198449755	25.915331203559433	25.40236217014585	24.811847427844967
18	24.666094848129923	24.805955065735972	26.650820289070392	23.877129797063716
19	25.37173300484341	25.13092439487943	25.526379716043735	23.97096288423343
20	25.489580247023387	24.515283731132627	25.29574376896604	24.699392252877946
21	26.436638485240465	24.454803637032718	24.608394170119162	24.50016370760765
22	25.524545301424894	24.751923216962886	25.110356715819716	24.613174765792504
23	24.312052826693957	24.52395550933078	25.717436778011926	25.446554885963334
24	24.584546780074255	25.12208585171593	25.574852732638515	24.7185146355713
25	24.702671963863143	24.674043978144898	25.65200932327333	24.971274734718634
26	24.09431337026992	25.613986911173754	26.058693485437807	24.23300623311852
27	24.966382962401727	25.06210605251206	25.302247602614653	24.669263382471556
28	24.17069172439977	24.973998562486	25.938789475351857	24.91652023776237
29	24.120328704864367	25.153660018488676	25.76991215377509	24.95609912287187
30	24.134726080206164	25.322982046639712	26.027564025239318	24.5147278479148
31	24.604892105846833	25.067442531403234	25.181454179380182	25.146211183369754
32	24.358024368808504	25.178063291751418	25.20146597522206	25.26244636421802
33	24.045951530318703	24.81646125855296	25.816661932394595	25.320925278733743
34	24.800785351810152	24.970941204787934	26.019281365293658	24.208992078108263
35	25.237209266128534	24.79761681746852	25.85657434743479	24.108599568968152
36	24.061905378670428	25.37262241799194	25.548670633078718	25.016801570258913
37	24.960268247005597	25.060438402858576	25.318757334184205	24.660536015951624
38	24.18875792897925	24.933474675906186	25.85062639700401	25.027140998110553
39	24.896119323668007	24.576708826702852	25.39780392775964	25.1293679218695
40	25.039815135477077	24.77654884351276	25.973643353109804	24.209992667900355
41	23.833326199498707	25.048375737031662	26.531805692132977	24.58649237133666
42	25.039981900442427	25.352610622150056	25.831059307736393	23.77634816967112
43	24.073856867853774	24.41655887164601	26.188881335053605	25.320702925446607
44	23.65850092749115	25.00790743877363	25.985539253971368	25.348052379763853
45	23.810145869315193	25.207636278939976	26.116727693379264	24.86549015836557
46	23.6373905048423	24.699906278037375	26.265051936197914	25.39765128092241
47	23.902589248440332	24.573317939074087	26.537864819207325	24.98622799327826
48	24.40160561308638	25.306972609966206	25.634832531842385	24.65658924510503
49	23.53298249690512	25.24393545306428	26.05324582990307	25.16983622012753
50	23.37258058508997	25.65965345089906	25.769440508478063	25.198325455532906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	69.0
1	71.5
2	74.0
3	202.5
4	331.0
5	369.0
6	407.0
7	355.0
8	303.0
9	442.0
10	581.0
11	862.0
12	1143.0
13	1905.0
14	2667.0
15	3509.0
16	4351.0
17	5037.0
18	5723.0
19	6186.5
20	6650.0
21	7739.5
22	8829.0
23	9611.0
24	10393.0
25	12265.0
26	14137.0
27	17516.0
28	20895.0
29	24188.0
30	27481.0
31	30254.5
32	33028.0
33	38018.0
34	43008.0
35	49315.5
36	55623.0
37	64070.0
38	72517.0
39	76679.5
40	80842.0
41	86646.0
42	92450.0
43	94784.5
44	97119.0
45	105369.5
46	113620.0
47	121014.0
48	128408.0
49	132171.0
50	135934.0
51	129883.0
52	123832.0
53	121759.0
54	119686.0
55	121113.0
56	122540.0
57	119177.5
58	115815.0
59	105909.5
60	96004.0
61	84646.5
62	73289.0
63	64391.5
64	55494.0
65	47775.0
66	40056.0
67	34804.0
68	29552.0
69	26969.0
70	24386.0
71	19126.5
72	13867.0
73	12420.5
74	10974.0
75	8134.0
76	5294.0
77	4779.0
78	4264.0
79	3627.0
80	2990.0
81	2399.5
82	1809.0
83	1528.5
84	1248.0
85	1012.0
86	776.0
87	561.5
88	347.0
89	218.5
90	90.0
91	59.0
92	28.0
93	17.0
94	6.0
95	5.0
96	4.0
97	2.5
98	1.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01600943667350588
2	0.004280300777291504
3	3.8911825248104577E-4
4	2.779416089150327E-4
5	0.0
6	7.782365049620915E-4
7	0.0
8	5.5588321783006535E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	5.5588321783006535E-5
47	0.0
48	0.0
49	0.0
50	1.1117664356601307E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1798939.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.18648526618896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.76094830433115	46.41009243327224
2	12.966451532512552	15.089444821025896
3	3.495950545712113	6.102512247582093
4	1.3475500643952039	3.1363680786953405
5	0.6856732838593785	1.9948459214351562
6	0.3881350382800908	1.3550528211705715
7	0.25250223478408484	1.0284552294760756
8	0.163560032142655	0.761358672032479
9	0.13569703702921265	0.7106160281189201
>10	0.6544618266210764	6.963402860872138
>50	0.0706486381304922	2.9215051701930994
>100	0.06774138008301607	8.412595631053025
>500	0.00788979039419111	2.980207623395921
>1k	0.0027902917247749045	2.1335424616770955
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2149	0.11945930351168106	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1919	0.10667398950158956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.558832178300654E-5	0.0
3	0.0	0.0	0.0	5.558832178300654E-5	0.0
4	0.0	0.0	0.0	5.558832178300654E-5	0.0
5	0.0	0.0	0.0	5.558832178300654E-5	5.558832178300654E-5
6	0.0	0.0	0.0	5.558832178300654E-5	5.558832178300654E-5
7	0.0	0.0	0.0	5.558832178300654E-5	5.558832178300654E-5
8	0.0	0.0	0.0	1.1117664356601308E-4	5.558832178300654E-5
9	0.0	0.0	0.0	1.6676496534901963E-4	1.1117664356601308E-4
10	0.0	0.0	0.0	1.6676496534901963E-4	1.6676496534901963E-4
11	0.0	0.0	0.0	1.6676496534901963E-4	2.2235328713202617E-4
12	0.0	0.0	0.0	1.6676496534901963E-4	3.8911825248104577E-4
13	0.0	5.558832178300654E-5	0.0	1.6676496534901963E-4	4.4470657426405233E-4
14	0.0	5.558832178300654E-5	0.0	1.6676496534901963E-4	4.4470657426405233E-4
15	0.0	5.558832178300654E-5	0.0	1.6676496534901963E-4	5.558832178300654E-4
16	0.0	5.558832178300654E-5	0.0	2.779416089150327E-4	5.558832178300654E-4
17	0.0	5.558832178300654E-5	0.0	3.8911825248104577E-4	5.558832178300654E-4
18	0.0	5.558832178300654E-5	0.0	6.114715396130719E-4	6.670598613960785E-4
19	0.0	5.558832178300654E-5	0.0	6.114715396130719E-4	6.670598613960785E-4
20	0.0	5.558832178300654E-5	0.0	7.782365049620915E-4	6.670598613960785E-4
21	0.0	5.558832178300654E-5	0.0	0.0010005897920941178	6.670598613960785E-4
22	0.0	5.558832178300654E-5	0.0	0.0017788262970562093	6.670598613960785E-4
23	0.0	5.558832178300654E-5	0.0	0.00289059273271634	7.22648183179085E-4
24	0.0	5.558832178300654E-5	0.0	0.0041691241337254905	7.22648183179085E-4
25	0.0	5.558832178300654E-5	0.0	0.005503243856517647	7.22648183179085E-4
26	0.0	5.558832178300654E-5	0.0	0.006948540222875817	7.22648183179085E-4
27	0.0	5.558832178300654E-5	0.0	0.009283249737762091	7.22648183179085E-4
28	0.0	5.558832178300654E-5	0.0	0.019845030876533335	7.22648183179085E-4
29	0.0	5.558832178300654E-5	0.0	0.04413712749570719	7.22648183179085E-4
30	0.0	5.558832178300654E-5	0.0	0.08899690317459347	7.22648183179085E-4
31	0.0	1.1117664356601308E-4	0.0	0.178382924601668	7.22648183179085E-4
32	0.0	1.1117664356601308E-4	0.0	0.2868913287220968	7.22648183179085E-4
33	0.0	1.1117664356601308E-4	0.0	0.39139737367414906	7.22648183179085E-4
34	0.0	1.1117664356601308E-4	0.0	0.5103563822897831	7.782365049620915E-4
35	0.0	1.1117664356601308E-4	0.0	0.646047475762102	7.782365049620915E-4
36	0.0	1.1117664356601308E-4	0.0	0.8425521932650301	7.782365049620915E-4
37	0.0	1.6676496534901963E-4	0.0	1.1217723335810719	7.782365049620915E-4
38	0.0	1.6676496534901963E-4	0.0	1.4770928864180497	8.338248267450981E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	565	0.0	19.858206	36
TAGGACG	655	0.0	19.144844	4
CTAGCGG	615	0.0	18.601437	29
ATACGAA	620	0.0	18.451426	40
TAGCGGC	610	0.0	18.393255	30
TCTAGCG	640	0.0	17.874819	28
CGAATGC	640	0.0	17.874819	43
CAATACG	640	0.0	17.531073	38
CGTCTTA	165	1.6607373E-9	17.333158	15
CGGTCCA	695	0.0	17.093353	10
GTCCTAA	440	0.0	17.0055	1
GTATCAA	3750	0.0	16.960152	1
AATACGA	675	0.0	16.947977	39
CGAGCCG	565	0.0	16.743195	15
CGTCGTA	530	0.0	16.603605	10
TCCTACA	800	0.0	16.501667	2
GCAATAC	695	0.0	16.143723	37
GATTTCG	355	0.0	16.112513	41
GACGTGA	455	0.0	15.955883	7
GCGAAAG	745	0.0	15.946147	18
>>END_MODULE