FastQCFastQC Report
Thu 2 Feb 2017
SRR4062673_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062673_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623619
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG88220.5433540750631768No Hit
TCCATGTACTCTGCGTTGATACCAC77380.4765896432599027No Hit
GTATCAACGCAGAGTACTTTTTTTT52570.3237828579241805No Hit
GAGTACATGGAAGCAGTGGTATCAA35790.22043348839844817No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35650.21957121713899627No Hit
TATCAACGCAGAGTACTTTTTTTTT33840.20842328157036843No Hit
CATGTACTCTGCGTTGATACCACTG31650.19493489544037118No Hit
CCCATGTACTCTGCGTTGATACCAC25600.15767245887120068No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24680.1520061048805169No Hit
GCTTCCATGTACTCTGCGTTGATAC24460.15065110718709254No Hit
GTACATGGGAAGCAGTGGTATCAAC23870.14701724973654534No Hit
GCGTTGATACCACTGCTTCCATGTA21570.1328513647598359No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG21350.13149636706641152No Hit
CATGGAAGCAGTGGTATCAACGCAG20910.12878637167956275No Hit
GAATAGGACCGCGGTTCTATTTTGT20180.12429024296956366No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20060.12355115331860493No Hit
GTATCAACGCAGAGTACATGGAAGC20050.12348956251435836No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20040.12342797171011179No Hit
GTATCTGATCGTCTTCGAACCTCCG19570.12053320391052336No Hit
GCGCAAGACGGACCAGAGCGAAAGC19490.12004047747655085No Hit
GATTAAGAGGGACGGCCGGGGGCAT18550.11425094187737393No Hit
GTCCTATTCCATTATTCCTAGCTGC18410.11338867061792206No Hit
GGTATCAACGCAGAGTACTTTTTTT18290.11264958096696331No Hit
CTATTGGAGCTGGAATTACCGCGGC18040.11110981086079924No Hit
GTCCTACAGTGGACATTTCTAAATT17240.1061825465210742No Hit
GTCCTAAAGTGTGTATTTCTCATTT16850.10378050515545828No Hit
GAATAACGCCGCCGCATCGCCAGTC16760.1032261879172392No Hit
ACGCAGAGTACATGGAAGCAGTGGT16530.10180959941956827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA601.465196E-615.83510610
GCGCTAT601.465196E-615.83510611
CGAACGA1058.913048E-1114.47781216
CACCGGT500.001498679913.301087
AGGATCG500.001500422513.2990285
CTTATAC1001.0108124E-813.2965683
GAGCGTT655.4486216E-513.1549147
TCTAGTA655.475487E-513.148022
ACGAACG1207.403287E-1012.66769515
CGGTCCA3800.012.25137110
CGCTATC701.0898168E-412.21565312
CGGCTAA701.0898168E-412.2156539
CGTCTTA1950.012.18047615
CGGTGTT550.003065970312.09226314
CCAACGT550.003065970312.09226319
GACCATA3250.011.69361811
ATCCCCG2950.011.59451912
TCTCGTC2050.011.58666312
CCGACCA3200.011.5794229
ACGGTAT3200.011.5794229
Produced by FastQC (version 0.11.5)