##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062673_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1623619
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.930414709362232	32.0	32.0	32.0	32.0	32.0
2	30.595045389343188	32.0	32.0	32.0	32.0	32.0
3	30.60305404161937	32.0	32.0	32.0	32.0	32.0
4	30.630330761096044	32.0	32.0	32.0	32.0	32.0
5	30.459878210343682	32.0	32.0	32.0	32.0	32.0
6	34.059802823199284	36.0	36.0	36.0	32.0	36.0
7	33.8858777829035	36.0	36.0	36.0	32.0	36.0
8	33.88354780277886	36.0	36.0	36.0	32.0	36.0
9	34.02379006404828	36.0	36.0	36.0	32.0	36.0
10	33.69106668497967	36.0	36.0	36.0	27.0	36.0
11	34.06292301334241	36.0	36.0	36.0	32.0	36.0
12	33.798974389927686	36.0	36.0	36.0	32.0	36.0
13	33.94306730827861	36.0	36.0	36.0	32.0	36.0
14	33.84088385267726	36.0	36.0	36.0	32.0	36.0
15	33.805419867592086	36.0	36.0	36.0	32.0	36.0
16	33.795474184522355	36.0	36.0	36.0	32.0	36.0
17	33.700788793429986	36.0	36.0	36.0	27.0	36.0
18	33.74119359283181	36.0	36.0	36.0	32.0	36.0
19	33.70882700929221	36.0	36.0	36.0	27.0	36.0
20	33.68500922938202	36.0	36.0	36.0	27.0	36.0
21	33.62969514399622	36.0	36.0	36.0	27.0	36.0
22	33.5796760200515	36.0	36.0	36.0	27.0	36.0
23	33.565048204042945	36.0	36.0	36.0	27.0	36.0
24	33.55255019804523	36.0	36.0	36.0	27.0	36.0
25	33.0647966056076	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	6.0
4	72.0
5	158.0
6	269.0
7	74.0
8	224.0
9	279.0
10	200.0
11	65.0
12	145.0
13	96.0
14	523.0
15	584.0
16	881.0
17	1207.0
18	1678.0
19	2602.0
20	3996.0
21	5743.0
22	8087.0
23	11127.0
24	14411.0
25	18901.0
26	24469.0
27	30681.0
28	39336.0
29	50304.0
30	63232.0
31	84100.0
32	116909.0
33	164378.0
34	352166.0
35	626714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.724218260122925	18.21626689103871	12.060469579807503	24.999045269030866
2	15.476957455274269	20.57513048469969	38.667386893755825	25.280525166270213
3	19.150666048261474	25.37376865609588	28.049910965666808	27.425654329975835
4	12.158280516283709	16.20504493427565	36.07228415439231	35.56439039504833
5	13.8068430719334	37.613051533446736	33.29369585402235	15.286409540597507
6	32.996545651501464	35.511392695217516	17.708373128791866	13.783688524489149
7	29.467870774339517	31.29344608540717	21.00628822284826	18.23239491740506
8	27.715873659708723	33.41903882452586	19.895920533687743	18.969166982077667
9	27.181520023673304	15.487892089169462	19.35366936279345	37.97691852436378
10	16.251919270399764	26.845428590808467	31.14151112097724	25.76114101781453
11	36.03864150422156	21.847459048539903	22.74578892454473	19.368110522693804
12	25.120446707419962	24.319318837791034	28.67112683352822	21.889107621260788
13	29.353784830093645	20.194640300235932	25.591154238439916	24.860420631230507
14	23.568128447246643	21.043374872963717	25.164283811384426	30.224212868405214
15	24.802867516118372	28.0388740078631	23.431819415052363	23.726439060966165
16	24.96237217174691	26.259753792841927	25.310996081526447	23.466877953884715
17	23.03362748906937	26.79616919196591	26.3214499349404	23.84875338402432
18	24.351886170508656	24.663632052286967	28.324903963150465	22.65957781405391
19	24.777578918934253	26.229591100443177	25.948858877786506	23.04397110283606
20	25.05135217633946	24.588488849285756	26.723481403143108	23.636677571231683
21	26.028994766683894	24.945884575963255	24.548914793029315	24.476205864323536
22	24.855273830856206	25.955531978546293	25.22482288971203	23.964371300885475
23	22.76227153354542	25.695313916640046	26.17836696982892	25.364047579985616
24	23.678059262219655	26.64838621342984	25.92722109973825	23.74633342461225
25	24.13497930610863	25.548861450527383	25.763478473234237	24.55268077012975
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	756.0
1	756.0
2	1284.5
3	1813.0
4	1813.0
5	1813.0
6	3822.5
7	5832.0
8	5832.0
9	5832.0
10	5858.0
11	5884.0
12	5884.0
13	5884.0
14	5911.5
15	5939.0
16	5939.0
17	5939.0
18	9490.5
19	13042.0
20	13042.0
21	13042.0
22	21502.0
23	29962.0
24	29962.0
25	29962.0
26	44861.5
27	59761.0
28	59761.0
29	59761.0
30	73414.0
31	87067.0
32	87067.0
33	87067.0
34	101409.0
35	115751.0
36	115751.0
37	115751.0
38	124647.0
39	133543.0
40	133543.0
41	133543.0
42	151716.0
43	169889.0
44	169889.0
45	169889.0
46	205703.5
47	241518.0
48	241518.0
49	241518.0
50	225607.5
51	209697.0
52	209697.0
53	209697.0
54	184086.5
55	158476.0
56	158476.0
57	158476.0
58	145425.5
59	132375.0
60	132375.0
61	132375.0
62	117526.0
63	102677.0
64	102677.0
65	102677.0
66	85334.5
67	67992.0
68	67992.0
69	67992.0
70	51819.0
71	35646.0
72	35646.0
73	35646.0
74	28372.0
75	21098.0
76	21098.0
77	21098.0
78	17898.5
79	14699.0
80	14699.0
81	14699.0
82	10140.5
83	5582.0
84	5582.0
85	5582.0
86	4163.5
87	2745.0
88	2745.0
89	2745.0
90	1856.0
91	967.0
92	967.0
93	967.0
94	580.5
95	194.0
96	194.0
97	194.0
98	454.0
99	714.0
100	714.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007698850530820346
2	0.003510675842054078
3	0.005419990773697524
4	0.015274519453147567
5	0.035476303246020156
6	0.04539242272971676
7	0.06540943410984966
8	0.0860423535324482
9	0.09478824773546012
10	0.11757684530668833
11	0.11967093265107147
12	0.13272818315134277
13	0.12077956712750959
14	0.12533728664175525
15	0.11499003152833269
16	0.11172571890326487
17	0.12496774181627586
18	0.12971023374326118
19	0.13211227510887713
20	0.12158024758271492
21	0.12841682685408337
22	0.15114383362106504
23	0.13950317161846468
24	0.13642363140613656
25	0.13094204982819246
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1623619.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.9568981913086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.04080168724717	34.63515478030367
2	13.47912486375874	12.119592884158763
3	4.248446426256516	5.729909203693293
4	1.7612587288617119	3.1672291744795835
5	0.9248581393859071	2.078937660688766
6	0.5350516995716043	1.443255887083635
7	0.34494265596865	1.0855286306355534
8	0.24287049472373415	0.8734963283974136
9	0.16855556267331118	0.6819961743614509
>10	0.9756280268316998	8.190714997384644
>50	0.11904292443633484	3.782028195471051
>100	0.13172961901404023	12.760158553840592
>500	0.018779289426107793	5.7414514969998764
>1k	0.008498656528603526	6.366079613666445
>5k	4.1122531590017064E-4	1.3444664188352904
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	8822	0.5433540750631768	No Hit
TCCATGTACTCTGCGTTGATACCAC	7738	0.4765896432599027	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5257	0.3237828579241805	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3579	0.22043348839844817	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3565	0.21957121713899627	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3384	0.20842328157036843	No Hit
CATGTACTCTGCGTTGATACCACTG	3165	0.19493489544037118	No Hit
CCCATGTACTCTGCGTTGATACCAC	2560	0.15767245887120068	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2468	0.1520061048805169	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2446	0.15065110718709254	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2387	0.14701724973654534	No Hit
GCGTTGATACCACTGCTTCCATGTA	2157	0.1328513647598359	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2135	0.13149636706641152	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2091	0.12878637167956275	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2018	0.12429024296956366	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2006	0.12355115331860493	No Hit
GTATCAACGCAGAGTACATGGAAGC	2005	0.12348956251435836	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2004	0.12342797171011179	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1957	0.12053320391052336	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1949	0.12004047747655085	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1855	0.11425094187737393	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1841	0.11338867061792206	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1829	0.11264958096696331	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1804	0.11110981086079924	No Hit
GTCCTACAGTGGACATTTCTAAATT	1724	0.1061825465210742	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1685	0.10378050515545828	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1676	0.1032261879172392	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1653	0.10180959941956827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	6.159080424656278E-5	0.0
9	0.0	0.0	0.0	6.159080424656278E-5	0.0
10	0.0	0.0	0.0	6.159080424656278E-5	0.0
11	0.0	0.0	0.0	1.2318160849312555E-4	0.0
12	0.0	0.0	0.0	1.2318160849312555E-4	6.159080424656278E-5
13	0.0	0.0	0.0	1.2318160849312555E-4	6.159080424656278E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCTA	60	1.465196E-6	15.835106	10
GCGCTAT	60	1.465196E-6	15.835106	11
CGAACGA	105	8.913048E-11	14.477812	16
CACCGGT	50	0.0014986799	13.30108	7
AGGATCG	50	0.0015004225	13.299028	5
CTTATAC	100	1.0108124E-8	13.296568	3
GAGCGTT	65	5.4486216E-5	13.154914	7
TCTAGTA	65	5.475487E-5	13.14802	2
ACGAACG	120	7.403287E-10	12.667695	15
CGGTCCA	380	0.0	12.251371	10
CGCTATC	70	1.0898168E-4	12.215653	12
CGGCTAA	70	1.0898168E-4	12.215653	9
CGTCTTA	195	0.0	12.180476	15
CGGTGTT	55	0.0030659703	12.092263	14
CCAACGT	55	0.0030659703	12.092263	19
GACCATA	325	0.0	11.693618	11
ATCCCCG	295	0.0	11.594519	12
TCTCGTC	205	0.0	11.586663	12
CCGACCA	320	0.0	11.579422	9
ACGGTAT	320	0.0	11.579422	9
>>END_MODULE