FastQCFastQC Report
Thu 2 Feb 2017
SRR4062673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623619
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA51460.316946278652812No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC40770.2511057089132364No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC21590.132974546368329No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21430.131989093500384No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA21080.12983341535175433No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20300.12502933262052243No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19530.1202868406935371No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18580.11443571429011364No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA18480.113819806247648No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA17490.10772231662723827No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC17400.10716799938901922No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC17200.10593618330408798No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG16950.1043964131979239No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA16840.10371891435121171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG250.002353094535.1998024
TAGATCG703.216369E-521.9998765
GTATTAC1259.949872E-1021.1244351
TGTAGGA14400.019.0977042
TAGGACG12850.019.0037844
ACGATTA1051.7935563E-618.85703740
GTCTTAA2250.018.5816781
AGGACGT13500.018.5776755
CTGTAGG14300.018.4654161
GGACGTG13400.018.3879596
CACCGGT600.00741341118.3332317
GTCCTAA7250.018.210721
TATAGGA1701.2732926E-1018.1181052
GTCCTAC11950.018.0456311
CGCAATA6600.017.999936
TGGTATA4300.017.90687844
CGGTCCA6900.017.8549710
GACGTGA7150.017.8460547
AATCACG8850.017.6496234
TCCTACA13000.017.6004432
Produced by FastQC (version 0.11.5)