FastQCFastQC Report
Thu 2 Feb 2017
SRR4062672_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062672_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668481
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG44960.269466658595453No Hit
TCCATGTACTCTGCGTTGATACCAC35960.21552537907234184No Hit
GTATCAACGCAGAGTACTTTTTTTT30310.1816622424828332No Hit
GCGCAAGACGGACCAGAGCGAAAGC24010.1439033468166554No Hit
GTCTTGCGCCGGTCCAAGAATTTCA23740.14228510843096207No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG21970.13167665679141685No Hit
GAATAGGACCGCGGTTCTATTTTGT21230.12724148491951662No Hit
TATCAACGCAGAGTACTTTTTTTTT20890.12520370324864352No Hit
GAGTACATGGAAGCAGTGGTATCAA20530.12304605206771908No Hit
GGGTAGGCACACGCTGAGCCAGTCA19940.11950990152120403No Hit
GAACTACGACGGTATCTGATCGTCT19580.11735225034027957No Hit
GAATAACGCCGCCGCATCGCCAGTC19390.11621348999479167No Hit
GTATCTGATCGTCTTCGAACCTCCG18820.11279720895832797No Hit
GTCCTATTCCATTATTCCTAGCTGC17760.10644412492560598No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17750.10638419017058029No Hit
GATTAAGAGGGACGGCCGGGGGCAT17700.10608451639545191No Hit
CATGTACTCTGCGTTGATACCACTG17570.10530536458011808No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC16740.10033077991298672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG250.006037704618.997035
GCGCGCT953.0559022E-1015.00080615
GCGAATT456.763016E-414.77768413
CGAACGA1301.8189894E-1213.887444516
CGGCTAA551.9598917E-413.8185099
ACGCGCG1001.0073563E-813.29991613
TACACCG655.460954E-513.151795
TCGCCAG3200.013.06751417
CGCATCG3200.013.06241813
CGCCAGT3350.013.05017618
AAGACGG4750.012.9979695
GCATCGC3350.012.76149714
GGGTATA905.4377233E-712.6605091
CGGTCCA4500.012.4558510
AGGACGT2850.012.3314065
TAGGACG2950.012.2350084
CAAGACG4900.012.212014
CGCGCTA1103.803325E-812.09337316
CGAATTG550.00306806112.09119514
ATTCCGA1650.012.090476
Produced by FastQC (version 0.11.5)