FastQCFastQC Report
Thu 2 Feb 2017
SRR4062672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668481
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC25110.15049616986948008No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23480.1407268048002944No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20940.12550337702377193No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20570.12328579108782178No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA19320.11579394670961192No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19010.11393596930381587No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG18490.11081936204248057No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18240.10932099316683858No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC17350.10398679996955315No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17330.1038669304595018No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG17260.10344738717432203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG1900.023.1659091
ACCGTCG5850.019.931358
ATACCGT6150.018.9590936
CGTCGTA6050.018.90883410
TACCGTC6150.018.6013747
GTCGTAG6250.018.30375111
GTCCTAC4600.018.1802021
ATAGGAG2300.018.1747553
CCGTCGT6350.018.0155039
ACGGCGT1002.4842222E-517.599768
GTAGGAC5800.017.4490853
GGCGTTA4300.017.39511142
GTGTACG902.2101568E-417.1170331
TAGCGGC6950.017.09329230
TGTAGGA6350.016.97922
AATAACG6100.016.9536382
CGCAATA6500.016.92284636
TAGGACG4300.016.8834924
CTAGCGG7050.016.85083429
CTGTAGG6600.016.6724361
Produced by FastQC (version 0.11.5)