FastQCFastQC Report
Thu 2 Feb 2017
SRR4062671_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062671_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512208
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG69300.45827029085945853No Hit
TCCATGTACTCTGCGTTGATACCAC60670.40120142202659953No Hit
GTATCAACGCAGAGTACTTTTTTTT44510.29433781596182534No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31560.20870144847798716No Hit
GAGTACATGGAAGCAGTGGTATCAA29800.1970628379164771No Hit
TATCAACGCAGAGTACTTTTTTTTT27130.17940653666691356No Hit
CATGTACTCTGCGTTGATACCACTG26250.17358723138615853No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25590.16922275242559223No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20120.13305047982817178No Hit
GCTTCCATGTACTCTGCGTTGATAC18560.12273443864865151No Hit
GCGTTGATACCACTGCTTCCATGTA16800.11109582808714144No Hit
CATGGAAGCAGTGGTATCAACGCAG16030.10600393596648079No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTA400.005275960514.2512428
TGTACCG1051.371518E-913.5708135
GTATTAG2850.012.9938171
ACGCCCC1207.385097E-1012.66818917
AGGGGCG751.4792062E-512.6660945
CGGTCCA2750.012.43744810
CGCAAGA2600.012.4188132
TATTAGC1251.4169927E-912.15582752
GCGTTAT1251.4206307E-912.1542181
TCCAACG1353.7107384E-1011.96440118
CCGTCGT1850.011.8118399
CAAGACG3000.011.715364
CGGTCGG1302.6102498E-911.69332610
CGTCGTA1800.011.612123510
AGGACGT2400.011.4786465
CGCGCTA752.0714257E-411.40137116
TTCGGAA1751.8189894E-1211.40137118
GGTCCAA3100.011.33932211
GGACGTG2100.011.3101346
GAGTTAG855.3482465E-511.1711561
Produced by FastQC (version 0.11.5)