##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062671_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1512208 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.111458873382496 32.0 32.0 32.0 32.0 32.0 2 30.913606461544973 32.0 32.0 32.0 32.0 32.0 3 30.90049715383069 32.0 32.0 32.0 32.0 32.0 4 30.912842677726875 32.0 32.0 32.0 32.0 32.0 5 30.847850295726513 32.0 32.0 32.0 32.0 32.0 6 34.40679655179711 36.0 36.0 36.0 32.0 36.0 7 34.29814284807381 36.0 36.0 36.0 32.0 36.0 8 34.30112788716896 36.0 36.0 36.0 32.0 36.0 9 34.378890999121815 36.0 36.0 36.0 32.0 36.0 10 34.19288021224593 36.0 36.0 36.0 32.0 36.0 11 34.39095679959371 36.0 36.0 36.0 32.0 36.0 12 34.24119036534657 36.0 36.0 36.0 32.0 36.0 13 34.3148720281866 36.0 36.0 36.0 32.0 36.0 14 34.26229195983621 36.0 36.0 36.0 32.0 36.0 15 34.23357434956038 36.0 36.0 36.0 32.0 36.0 16 34.22261752351528 36.0 36.0 36.0 32.0 36.0 17 34.160417085480304 36.0 36.0 36.0 32.0 36.0 18 34.174860865701014 36.0 36.0 36.0 32.0 36.0 19 34.15788701025256 36.0 36.0 36.0 32.0 36.0 20 34.13839630527018 36.0 36.0 36.0 32.0 36.0 21 34.10044583813867 36.0 36.0 36.0 32.0 36.0 22 34.057430591558834 36.0 36.0 36.0 32.0 36.0 23 34.02771840910774 36.0 36.0 36.0 32.0 36.0 24 34.013458466031125 36.0 36.0 36.0 32.0 36.0 25 33.49593045401162 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 66.0 5 147.0 6 237.0 7 66.0 8 240.0 9 278.0 10 200.0 11 62.0 12 97.0 13 80.0 14 296.0 15 444.0 16 806.0 17 1113.0 18 1588.0 19 2106.0 20 2825.0 21 3517.0 22 4685.0 23 6273.0 24 8279.0 25 11099.0 26 15477.0 27 19718.0 28 27294.0 29 36848.0 30 48873.0 31 68321.0 32 99906.0 33 148998.0 34 350005.0 35 652256.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.633570137069285 17.51293911309043 11.116592883190197 25.73689786665009 2 16.148884306757118 20.837097279774415 37.507687709056086 25.506330704412385 3 19.455734625991152 24.88483133063956 28.313967714416087 27.3454663289532 4 12.448494757212718 15.897543753827756 36.59593831143492 35.0580231775246 5 14.005534236389822 36.999737378454846 34.06909261940846 14.925635765746875 6 32.68438135538275 36.03737709343082 17.675655005511032 13.602586545675397 7 28.868302967297588 30.9889734663073 21.609173800749485 18.533549765645628 8 27.463790801741773 33.07703559465289 20.571682729124156 18.887490874481177 9 27.310198745635372 14.495291995565044 18.945671780105577 39.24883747869401 10 15.266981501208601 27.362996988260907 32.74149029775103 24.628531212779468 11 36.71948914364028 21.780582029323263 22.30964542622674 19.190283400809715 12 23.574898804190994 24.4157196010306 29.913434790698663 22.095946804079748 13 29.432534895515396 20.296206349984043 25.5894266687677 24.681832085732864 14 23.18895659295876 19.985950099680135 25.953582360422978 30.87151094693812 15 24.835302334665126 27.16698830515824 23.884885418356234 24.1128239418204 16 24.999867592460184 26.32599530747679 24.931743913225397 23.74239318683763 17 23.186399335777818 25.995373221093555 26.53180943603352 24.2864180070951 18 24.02362036839617 25.242116721912407 27.75413151179098 22.98013139790044 19 24.483797297198866 26.229844703819534 26.119264129317028 23.16709386966457 20 24.427022503303856 24.808124707022717 27.015485090932206 23.749367698741224 21 25.619498706473852 25.084276191504113 25.037130523972444 24.259094578049588 22 24.325308809183046 25.70565323962374 25.94368019879291 24.025357752400303 23 23.640820997227934 25.15380122458224 26.06144367199844 25.143934106191384 24 23.45306638678555 26.222896173596926 26.16025401197882 24.163783427638702 25 24.193977727364828 25.304629736312023 25.9424285886449 24.558963947678244 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 249.0 1 249.0 2 692.5 3 1136.0 4 1136.0 5 1136.0 6 2943.0 7 4750.0 8 4750.0 9 4750.0 10 5074.0 11 5398.0 12 5398.0 13 5398.0 14 5624.5 15 5851.0 16 5851.0 17 5851.0 18 9396.0 19 12941.0 20 12941.0 21 12941.0 22 20194.5 23 27448.0 24 27448.0 25 27448.0 26 39608.5 27 51769.0 28 51769.0 29 51769.0 30 64783.5 31 77798.0 32 77798.0 33 77798.0 34 91400.5 35 105003.0 36 105003.0 37 105003.0 38 117080.5 39 129158.0 40 129158.0 41 129158.0 42 147491.5 43 165825.0 44 165825.0 45 165825.0 46 191927.0 47 218029.0 48 218029.0 49 218029.0 50 207454.5 51 196880.0 52 196880.0 53 196880.0 54 177797.0 55 158714.0 56 158714.0 57 158714.0 58 142041.5 59 125369.0 60 125369.0 61 125369.0 62 109781.0 63 94193.0 64 94193.0 65 94193.0 66 78043.5 67 61894.0 68 61894.0 69 61894.0 70 47240.5 71 32587.0 72 32587.0 73 32587.0 74 25403.0 75 18219.0 76 18219.0 77 18219.0 78 14590.5 79 10962.0 80 10962.0 81 10962.0 82 7720.0 83 4478.0 84 4478.0 85 4478.0 86 3277.0 87 2076.0 88 2076.0 89 2076.0 90 1384.5 91 693.0 92 693.0 93 693.0 94 406.0 95 119.0 96 119.0 97 119.0 98 394.0 99 669.0 100 669.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008332187106535608 2 0.0035709373313724036 3 0.0051580205897601384 4 0.014945034016484504 5 0.03484970321543068 6 0.04589315755504534 7 0.06844296551797106 8 0.08828150624781776 9 0.09806851967454212 10 0.1180393173425878 11 0.11922962978637859 12 0.1333811221736692 13 0.12180864008125866 14 0.12412313649974077 15 0.11327806756742459 16 0.11374096685112102 17 0.12366023721604436 18 0.13133113963158508 19 0.13205855279167944 20 0.12246992477225355 21 0.13192629585348048 22 0.15176483658332715 23 0.14164718081110533 24 0.13510046237025594 25 0.13318273676637077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1512208.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.09412969905784 #Duplication Level Percentage of deduplicated Percentage of total 1 76.7696527894808 41.527875549458116 2 14.451708756052032 15.635052156457766 3 4.346634473923351 7.053822269604172 4 1.6989371802128246 3.676101127079365 5 0.7953018545595941 2.1510580835223956 6 0.4426366724404505 1.4366427341131864 7 0.27534799832154144 1.0426297233506994 8 0.19398100554784817 0.8394586938607164 9 0.1280730665509194 0.6235200965665351 >10 0.7212160083188921 7.40372837191951 >50 0.08304979930589822 3.117393000913932 >100 0.08278981575865557 9.11488264535513 >500 0.007726971381719349 2.8481282900674323 >1k 0.002698307466632153 2.6674000599578607 >5k 2.453006787847412E-4 0.8623071977731647 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGAAGCAGTGGTATCAACG 6930 0.45827029085945853 No Hit TCCATGTACTCTGCGTTGATACCAC 6067 0.40120142202659953 No Hit GTATCAACGCAGAGTACTTTTTTTT 4451 0.29433781596182534 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3156 0.20870144847798716 No Hit GAGTACATGGAAGCAGTGGTATCAA 2980 0.1970628379164771 No Hit TATCAACGCAGAGTACTTTTTTTTT 2713 0.17940653666691356 No Hit CATGTACTCTGCGTTGATACCACTG 2625 0.17358723138615853 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2559 0.16922275242559223 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2012 0.13305047982817178 No Hit GCTTCCATGTACTCTGCGTTGATAC 1856 0.12273443864865151 No Hit GCGTTGATACCACTGCTTCCATGTA 1680 0.11109582808714144 No Hit CATGGAAGCAGTGGTATCAACGCAG 1603 0.10600393596648079 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.3225693819897793E-4 0.0 3 0.0 0.0 0.0 1.3225693819897793E-4 0.0 4 0.0 0.0 0.0 1.9838540729846688E-4 0.0 5 0.0 0.0 0.0 1.9838540729846688E-4 0.0 6 0.0 0.0 0.0 1.9838540729846688E-4 0.0 7 0.0 0.0 0.0 3.306423454974448E-4 0.0 8 0.0 0.0 0.0 3.306423454974448E-4 0.0 9 0.0 0.0 0.0 6.612846909948896E-4 6.612846909948896E-5 10 0.0 0.0 0.0 6.612846909948896E-4 6.612846909948896E-5 11 0.0 0.0 0.0 6.612846909948896E-4 6.612846909948896E-5 12 0.0 0.0 0.0 6.612846909948896E-4 1.3225693819897793E-4 13 0.0 0.0 0.0 6.612846909948896E-4 1.3225693819897793E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGCTA 40 0.0052759605 14.251242 8 TGTACCG 105 1.371518E-9 13.570813 5 GTATTAG 285 0.0 12.993817 1 ACGCCCC 120 7.385097E-10 12.668189 17 AGGGGCG 75 1.4792062E-5 12.666094 5 CGGTCCA 275 0.0 12.437448 10 CGCAAGA 260 0.0 12.418813 2 TATTAGC 125 1.4169927E-9 12.1558275 2 GCGTTAT 125 1.4206307E-9 12.154218 1 TCCAACG 135 3.7107384E-10 11.964401 18 CCGTCGT 185 0.0 11.811839 9 CAAGACG 300 0.0 11.71536 4 CGGTCGG 130 2.6102498E-9 11.693326 10 CGTCGTA 180 0.0 11.6121235 10 AGGACGT 240 0.0 11.478646 5 CGCGCTA 75 2.0714257E-4 11.401371 16 TTCGGAA 175 1.8189894E-12 11.401371 18 GGTCCAA 310 0.0 11.339322 11 GGACGTG 210 0.0 11.310134 6 GAGTTAG 85 5.3482465E-5 11.171156 1 >>END_MODULE