FastQCFastQC Report
Thu 2 Feb 2017
SRR4062671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512208
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA45300.299561965020685No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC35360.233830266735793No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC17100.11307968216012614No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA16720.11056680033434554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG3000.021.267184
TATACCG808.988811E-519.2504635
AGGACGT3650.017.4798745
CCGTCGT2600.016.9229249
CGTCGTA2251.8189894E-1216.62207410
TGGCGAG4500.016.13318818
TATGGCG4100.016.09741416
GACGTGA2600.016.0767787
CTACGAC3750.015.8403814
TACGACG3750.015.8403815
ATAACGC4450.015.8211283
GGACGTG4050.015.7534656
GATATAC4900.015.7162221
TAGAACA7700.015.71466354
AGACCGT1401.5863734E-615.7146636
TAGCGGC4350.015.6780230
GACGGTA3800.015.6314388
ATACACT5100.015.5297864
CGTATTG4550.015.47238727
ACGGTAT3850.015.42843159
Produced by FastQC (version 0.11.5)