##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062671_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1512208
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.211926533915967	32.0	32.0	32.0	32.0	32.0
2	31.33148217705501	32.0	32.0	32.0	32.0	32.0
3	31.436898230931195	32.0	32.0	32.0	32.0	32.0
4	31.561865827981336	32.0	32.0	32.0	32.0	32.0
5	31.477822495318105	32.0	32.0	32.0	32.0	32.0
6	35.05416715160877	36.0	36.0	36.0	36.0	36.0
7	35.07425499666712	36.0	36.0	36.0	36.0	36.0
8	35.00960185371324	36.0	36.0	36.0	36.0	36.0
9	35.13059248463174	36.0	36.0	36.0	36.0	36.0
10	35.014978098251035	36.0	36.0	36.0	36.0	36.0
11	35.14629535090411	36.0	36.0	36.0	36.0	36.0
12	35.04833197549544	36.0	36.0	36.0	36.0	36.0
13	35.09586776422291	36.0	36.0	36.0	36.0	36.0
14	35.05624292425381	36.0	36.0	36.0	36.0	36.0
15	35.022082279686394	36.0	36.0	36.0	36.0	36.0
16	35.04059229947203	36.0	36.0	36.0	36.0	36.0
17	35.01267153724884	36.0	36.0	36.0	36.0	36.0
18	35.026184889909324	36.0	36.0	36.0	36.0	36.0
19	34.99884275179076	36.0	36.0	36.0	36.0	36.0
20	34.9881550686149	36.0	36.0	36.0	36.0	36.0
21	34.96820080306413	36.0	36.0	36.0	36.0	36.0
22	34.95606622898437	36.0	36.0	36.0	32.0	36.0
23	34.915003094812356	36.0	36.0	36.0	32.0	36.0
24	34.90097790780104	36.0	36.0	36.0	32.0	36.0
25	34.872930840201875	36.0	36.0	36.0	32.0	36.0
26	34.83155293451694	36.0	36.0	36.0	32.0	36.0
27	34.82142932718251	36.0	36.0	36.0	32.0	36.0
28	34.797934543396146	36.0	36.0	36.0	32.0	36.0
29	34.77096470855861	36.0	36.0	36.0	32.0	36.0
30	34.75043248018791	36.0	36.0	36.0	32.0	36.0
31	34.742598240453695	36.0	36.0	36.0	32.0	36.0
32	34.70203305365399	36.0	36.0	36.0	32.0	36.0
33	34.67465917387026	36.0	36.0	36.0	32.0	36.0
34	34.65271311883021	36.0	36.0	36.0	32.0	36.0
35	34.61195880460889	36.0	36.0	36.0	32.0	36.0
36	34.5870859035265	36.0	36.0	36.0	32.0	36.0
37	34.566564916995546	36.0	36.0	36.0	32.0	36.0
38	34.51932339995556	36.0	36.0	36.0	32.0	36.0
39	34.47928591833928	36.0	36.0	36.0	32.0	36.0
40	34.451060965158234	36.0	36.0	36.0	32.0	36.0
41	34.438162607260374	36.0	36.0	36.0	32.0	36.0
42	34.36429181699872	36.0	36.0	36.0	32.0	36.0
43	34.369237565202674	36.0	36.0	36.0	32.0	36.0
44	34.33814660417085	36.0	36.0	36.0	32.0	36.0
45	34.25542980859776	36.0	36.0	36.0	32.0	36.0
46	34.266692148170094	36.0	36.0	36.0	32.0	36.0
47	34.23777020092474	36.0	36.0	36.0	32.0	36.0
48	34.19043412017395	36.0	36.0	36.0	32.0	36.0
49	34.18691145662501	36.0	36.0	36.0	32.0	36.0
50	33.59978058573953	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	7.0
21	30.0
22	134.0
23	371.0
24	959.0
25	2406.0
26	4921.0
27	9629.0
28	16801.0
29	25906.0
30	38503.0
31	56062.0
32	83285.0
33	132492.0
34	277635.0
35	863065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.12214053476332	18.119161690903944	11.606842875245702	26.151854899087034
2	15.660034850924673	21.320367290390205	37.091369601661206	25.928228257023918
3	18.908077331296557	25.110782670224406	28.707607153839287	27.273532844639746
4	12.298935857860448	16.32808800928975	36.768451875540606	34.6045242573092
5	13.872760890036293	37.2413715573519	33.84428597124205	15.041581581369758
6	33.02371902203671	36.14472650515407	17.322133786182288	13.509420686626935
7	28.97842095796345	30.964258885021106	21.461531746955444	18.595788410059992
8	27.70848983737687	33.14577095214415	20.36479108694042	18.78094812353856
9	27.49079491710135	14.270457503200618	18.72288732766921	39.51586025202882
10	15.307418027149705	27.482264344587513	32.58652248896977	24.623795139293005
11	37.058063441008116	21.66018166813031	22.2180414334536	19.063713457407978
12	23.70289007861352	24.32092675081735	29.790809200850678	22.18537396971845
13	29.806018748743558	20.081364468380013	25.45886544708135	24.653751335795075
14	23.27265825865225	19.955257477807287	25.728867986414567	31.043216277125897
15	25.00634833303355	27.226148783765197	23.541073714727073	24.22642916847418
16	25.274036375948285	26.259416693999764	24.73429581115825	23.732251118893696
17	23.380712177160813	25.987430300593566	26.363304518955065	24.268553003290553
18	24.279860971506565	25.291097520975953	27.465269328028945	22.963772179488537
19	24.788653412758034	26.105601874874356	25.847700845386353	23.25804386698126
20	24.707117010358363	24.870057558219504	26.742154518426037	23.680670912996096
21	25.910324505623567	25.18985483478463	24.748050532730947	24.151770126860857
22	24.503507454001035	25.735150190979017	25.689124776485773	24.07221757853417
23	23.83951149577307	25.077767079661	25.831565498926075	25.251155925639857
24	23.63100843270238	26.311393670711965	25.807825378519354	24.249772518066298
25	24.510583200194684	25.22649000666575	25.650042851247978	24.612883941891592
26	23.403989398283834	25.89954556516035	26.425994307661384	24.270470728894438
27	24.877794589104145	25.774099859278614	25.792681959095574	23.555423592521663
28	23.7445510141462	25.437572080031316	26.24599261477257	24.57188429104991
29	23.610111836466942	25.900339106789545	26.319130701596606	24.17041835514691
30	24.191579465258748	25.854842720049092	26.3590061684636	23.59457164622856
31	23.799437643498777	25.85649593177658	25.648918667273286	24.695147757451355
32	23.787203876715374	26.077166633161575	25.72999217038926	24.405637319733795
33	23.586107202183825	25.432612444848857	26.078687587950867	24.902592765016454
34	23.701236866886035	26.190312443790802	26.5140113000328	23.59443938929036
35	24.940021478526763	25.559050077767083	25.97823844338874	23.522690000317418
36	23.739988149778338	26.360460994783786	25.77495952937691	24.124591326060965
37	24.69223810481098	25.921037317617685	25.66234274650048	23.724381831070858
38	23.79494090760001	25.92659210902204	25.76047739464412	24.517989588733823
39	24.187810142520078	25.67708939510967	25.89551172854528	24.239588733824977
40	24.760152042576152	25.794004528477565	25.70526012294605	23.740583306000232
41	23.361931691936558	26.007268841323416	26.585562303598447	24.04523716314158
42	24.946303683091216	26.237924941542435	25.74797911398432	23.06779226138203
43	24.046691989461767	25.02420301969041	25.984190005607694	24.94491498524013
44	23.606673156073768	25.69944081766529	26.368528668013923	24.32535735824701
45	24.025001851597136	25.92156634537048	26.001714049919062	24.051717753113326
46	23.61588063009892	25.431901849416118	26.292630572401794	24.659586948083163
47	24.056214489012092	25.1678340545745	26.562417339413624	24.213534116999778
48	23.940754181964387	26.719075682710315	25.45026874609842	23.88990138922688
49	23.15852052098653	25.782233660977855	26.742485160771533	24.31676065726408
50	23.227095743442703	26.79214764106525	25.273837990540983	24.706918624951065
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	24.5
2	28.0
3	103.5
4	179.0
5	190.5
6	202.0
7	232.0
8	262.0
9	355.0
10	448.0
11	658.0
12	868.0
13	1434.5
14	2001.0
15	2904.0
16	3807.0
17	4674.0
18	5541.0
19	6370.5
20	7200.0
21	8384.5
22	9569.0
23	10578.5
24	11588.0
25	12761.5
26	13935.0
27	16956.0
28	19977.0
29	23304.5
30	26632.0
31	29189.5
32	31747.0
33	35973.0
34	40199.0
35	45679.0
36	51159.0
37	57520.5
38	63882.0
39	68007.0
40	72132.0
41	77295.5
42	82459.0
43	85379.0
44	88299.0
45	97577.0
46	106855.0
47	113774.5
48	120694.0
49	119423.5
50	118153.0
51	110434.5
52	102716.0
53	98362.0
54	94008.0
55	93013.5
56	92019.0
57	88625.0
58	85231.0
59	77151.5
60	69072.0
61	60879.0
62	52686.0
63	46095.0
64	39504.0
65	33853.5
66	28203.0
67	25112.5
68	22022.0
69	19986.0
70	17950.0
71	14198.5
72	10447.0
73	9172.0
74	7897.0
75	6025.5
76	4154.0
77	3638.5
78	3123.0
79	2704.5
80	2286.0
81	1789.0
82	1292.0
83	1094.5
84	897.0
85	709.0
86	521.0
87	381.5
88	242.0
89	152.5
90	63.0
91	42.5
92	22.0
93	14.5
94	7.0
95	6.0
96	5.0
97	3.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014019235449091661
2	0.0035048088622729153
3	8.596700982933565E-4
4	2.6451387639795585E-4
5	0.0
6	2.6451387639795585E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	6.612846909948896E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1512208.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.828962664289186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.66842656447486	44.91484539991103
2	14.064549897303042	16.266766618023397
3	4.098178206683626	7.10980183517733
4	1.6242942407897178	3.757250040257941
5	0.7812247876053333	2.2588709537423037
6	0.4321664901899954	1.4995043895572486
7	0.2475660956922785	1.0021543353312834
8	0.16563028115143333	0.7662581875825567
9	0.11614739259248938	0.6045014906807046
>10	0.6538945028428661	7.131158146773211
>50	0.07243671706818079	2.945970539967545
>100	0.06824665015736264	7.99858908563895
>500	0.005399907176098889	2.081053875874132
>1k	0.0018382662727145153	1.663275101482441
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	4530	0.299561965020685	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3536	0.233830266735793	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1710	0.11307968216012614	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1672	0.11056680033434554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.612846909948896E-5	0.0
5	0.0	0.0	0.0	6.612846909948896E-5	6.612846909948896E-5
6	0.0	0.0	0.0	1.3225693819897793E-4	6.612846909948896E-5
7	0.0	0.0	0.0	1.3225693819897793E-4	6.612846909948896E-5
8	0.0	0.0	0.0	1.3225693819897793E-4	6.612846909948896E-5
9	0.0	0.0	0.0	1.9838540729846688E-4	6.612846909948896E-5
10	0.0	0.0	0.0	1.9838540729846688E-4	6.612846909948896E-5
11	0.0	0.0	0.0	1.9838540729846688E-4	6.612846909948896E-5
12	0.0	0.0	0.0	1.9838540729846688E-4	1.3225693819897793E-4
13	0.0	0.0	0.0	1.9838540729846688E-4	1.9838540729846688E-4
14	0.0	0.0	0.0	1.9838540729846688E-4	1.9838540729846688E-4
15	0.0	0.0	0.0	2.6451387639795585E-4	2.6451387639795585E-4
16	1.3225693819897793E-4	0.0	0.0	2.6451387639795585E-4	2.6451387639795585E-4
17	1.3225693819897793E-4	0.0	0.0	3.306423454974448E-4	2.6451387639795585E-4
18	1.3225693819897793E-4	0.0	0.0	3.306423454974448E-4	2.6451387639795585E-4
19	1.3225693819897793E-4	0.0	0.0	5.290277527959117E-4	2.6451387639795585E-4
20	1.3225693819897793E-4	0.0	0.0	5.290277527959117E-4	3.9677081459693375E-4
21	1.3225693819897793E-4	0.0	0.0	5.951562218954006E-4	3.9677081459693375E-4
22	1.3225693819897793E-4	0.0	0.0	0.0011903124437908012	3.9677081459693375E-4
23	1.3225693819897793E-4	0.0	0.0	0.001587083258387735	3.9677081459693375E-4
24	1.9838540729846688E-4	0.0	0.0	0.002909652640377514	3.9677081459693375E-4
25	1.9838540729846688E-4	0.0	0.0	0.003438680393173426	3.9677081459693375E-4
26	1.9838540729846688E-4	0.0	0.0	0.004959635182461672	3.9677081459693375E-4
27	1.9838540729846688E-4	0.0	0.0	0.00634833303355094	3.9677081459693375E-4
28	1.9838540729846688E-4	0.0	0.0	0.010051527303122322	3.9677081459693375E-4
29	1.9838540729846688E-4	0.0	0.0	0.02023531154444362	3.9677081459693375E-4
30	1.9838540729846688E-4	0.0	0.0	0.03928031064509644	3.9677081459693375E-4
31	1.9838540729846688E-4	0.0	0.0	0.08226381555976427	4.628992836964227E-4
32	1.9838540729846688E-4	0.0	0.0	0.13695205950504163	4.628992836964227E-4
33	1.9838540729846688E-4	0.0	0.0	0.18661453979875783	4.628992836964227E-4
34	2.6451387639795585E-4	0.0	0.0	0.24837852993768053	4.628992836964227E-4
35	2.6451387639795585E-4	0.0	0.0	0.3192021203432332	4.628992836964227E-4
36	2.6451387639795585E-4	0.0	0.0	0.4206431919418493	4.628992836964227E-4
37	2.6451387639795585E-4	0.0	0.0	0.5785579761514289	4.628992836964227E-4
38	2.6451387639795585E-4	0.0	0.0	0.7824320463851534	4.628992836964227E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	300	0.0	21.26718	4
TATACCG	80	8.988811E-5	19.250463	5
AGGACGT	365	0.0	17.479874	5
CCGTCGT	260	0.0	16.922924	9
CGTCGTA	225	1.8189894E-12	16.622074	10
TGGCGAG	450	0.0	16.133188	18
TATGGCG	410	0.0	16.097414	16
GACGTGA	260	0.0	16.076778	7
CTACGAC	375	0.0	15.840381	4
TACGACG	375	0.0	15.840381	5
ATAACGC	445	0.0	15.821128	3
GGACGTG	405	0.0	15.753465	6
GATATAC	490	0.0	15.716222	1
TAGAACA	770	0.0	15.7146635	4
AGACCGT	140	1.5863734E-6	15.714663	6
TAGCGGC	435	0.0	15.67802	30
GACGGTA	380	0.0	15.631438	8
ATACACT	510	0.0	15.529786	4
CGTATTG	455	0.0	15.472387	27
ACGGTAT	385	0.0	15.4284315	9
>>END_MODULE