FastQCFastQC Report
Thu 2 Feb 2017
SRR4062670_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062670_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610423
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37860.23509351269821654No Hit
GTACATGGAAGCAGTGGTATCAACG30610.19007428483075567No Hit
TCCATGTACTCTGCGTTGATACCAC25040.15548709873120292No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24650.1530653747493671No Hit
TATCAACGCAGAGTACTTTTTTTTT22740.1412051367870429No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18770.11655322856168844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAAC1251.03682396E-1012.9153933
GCATCGC2100.012.66726714
TTATACG751.486463E-512.6601842
AGGACGT3600.012.4022095
CGCATCG2100.012.21486513
ATCGCCA2200.012.09336216
TCGTTAG550.00306743612.09148314
AAAATCG550.003069579112.0903555
TATCCCG550.003069579112.0903555
ACACGCT2700.011.9642749
CGCGCCA1052.716024E-711.76319410
GCGTATG658.016312E-411.693598
CGTCTTA658.020494E-411.692862515
GGACGTG3750.011.6546116
CGAGCGA907.451805E-611.61238410
CGGTCTG907.4569634E-611.61166315
ATATACG2300.011.5664866
CGCAAGA2950.011.2654182
TAGGACG4050.011.2569934
ATATATC1354.7530193E-911.2552453
Produced by FastQC (version 0.11.5)