##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062670_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1610423
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.136191547189775	32.0	32.0	32.0	32.0	32.0
2	30.814525748824998	32.0	32.0	32.0	32.0	32.0
3	30.80262142306711	32.0	32.0	32.0	32.0	32.0
4	30.865428524058586	32.0	32.0	32.0	32.0	32.0
5	30.682301482281364	32.0	32.0	32.0	32.0	32.0
6	34.31412305959366	36.0	36.0	36.0	32.0	36.0
7	34.197925638170844	36.0	36.0	36.0	32.0	36.0
8	34.19050895323775	36.0	36.0	36.0	32.0	36.0
9	34.33722630638037	36.0	36.0	36.0	32.0	36.0
10	34.027911300322955	36.0	36.0	36.0	32.0	36.0
11	34.333827199437664	36.0	36.0	36.0	32.0	36.0
12	34.11050823293011	36.0	36.0	36.0	32.0	36.0
13	34.2315546909104	36.0	36.0	36.0	32.0	36.0
14	34.15078522847724	36.0	36.0	36.0	32.0	36.0
15	34.0837165142326	36.0	36.0	36.0	32.0	36.0
16	34.0860972551932	36.0	36.0	36.0	32.0	36.0
17	34.00943106252208	36.0	36.0	36.0	32.0	36.0
18	34.02520021137304	36.0	36.0	36.0	32.0	36.0
19	34.01616159232699	36.0	36.0	36.0	32.0	36.0
20	34.0051433691645	36.0	36.0	36.0	32.0	36.0
21	33.97038355761188	36.0	36.0	36.0	32.0	36.0
22	33.927702845774064	36.0	36.0	36.0	32.0	36.0
23	33.890583405726325	36.0	36.0	36.0	32.0	36.0
24	33.87275082385187	36.0	36.0	36.0	32.0	36.0
25	33.42261132634097	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	62.0
5	164.0
6	264.0
7	54.0
8	260.0
9	286.0
10	181.0
11	52.0
12	124.0
13	88.0
14	299.0
15	366.0
16	554.0
17	807.0
18	1068.0
19	1559.0
20	2248.0
21	3410.0
22	5124.0
23	7615.0
24	10162.0
25	14280.0
26	19426.0
27	25129.0
28	33811.0
29	45034.0
30	57945.0
31	78268.0
32	111268.0
33	160765.0
34	357900.0
35	671841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.9710883656692	17.167074668723444	10.719462257015214	26.142374708592136
2	16.947503270993504	20.066171251646363	36.546886517172794	26.439438960187335
3	19.653529166294078	23.752384571820322	27.90334038769163	28.69074587419396
4	12.822996994770191	15.073037828384548	35.47034423823728	36.63362093860798
5	14.693241415809805	36.376517300690935	33.482643403201166	15.447597880298089
6	34.5903725083588	34.730897158569206	16.845066292596922	13.833664040475075
7	30.202369427353176	30.227535117011133	20.800281358624126	18.769814097011558
8	28.314346266912782	32.68031498020497	19.67383265278218	19.331506100100064
9	27.420987892962827	14.271374399511716	18.505512154585215	39.80212555294024
10	15.927026539759906	26.72091910004787	31.81252447887201	25.53952988132021
11	37.57914068017467	21.04849900032825	22.111209752021725	19.261150567475358
12	24.69996306663798	23.656502323568585	28.794094641429286	22.84943996836415
13	29.824106575549248	19.453220878920018	25.119709737303804	25.602962808226927
14	23.332779992178615	19.798981727928993	24.991062607753864	31.877175672138524
15	25.150162938133242	27.20762209791705	22.529351865551483	25.11286309839822
16	25.662957082628278	25.62516085155903	24.164348953703346	24.547533112109342
17	23.960590312209927	25.743252611075224	25.48125913542153	24.814897941293317
18	24.738439384528146	24.604262690360855	26.7536390298739	23.9036588952371
19	25.333750333423904	25.18894021250839	25.375160183372262	24.102149270695445
20	25.368983731143597	24.42833944265116	25.583099985576375	24.61957684062887
21	26.33994909169588	24.381459130977593	24.455197832355747	24.823393944970785
22	25.45908972468609	24.83757965546427	25.038136987187343	24.665193632662298
23	24.19468370613386	24.546756971199883	25.559946971373993	25.69861235129226
24	24.558209521322404	25.28780657657069	25.420556679941676	24.733427222165226
25	24.679059315969035	24.83226275384423	25.45123200061679	25.03744592956994
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	271.0
1	271.0
2	615.0
3	959.0
4	959.0
5	959.0
6	2548.5
7	4138.0
8	4138.0
9	4138.0
10	4329.0
11	4520.0
12	4520.0
13	4520.0
14	4818.0
15	5116.0
16	5116.0
17	5116.0
18	8173.5
19	11231.0
20	11231.0
21	11231.0
22	17635.5
23	24040.0
24	24040.0
25	24040.0
26	35811.0
27	47582.0
28	47582.0
29	47582.0
30	59255.0
31	70928.0
32	70928.0
33	70928.0
34	85693.5
35	100459.0
36	100459.0
37	100459.0
38	113550.0
39	126641.0
40	126641.0
41	126641.0
42	144890.5
43	163140.0
44	163140.0
45	163140.0
46	187408.5
47	211677.0
48	211677.0
49	211677.0
50	211885.5
51	212094.0
52	212094.0
53	212094.0
54	198473.0
55	184852.0
56	184852.0
57	184852.0
58	169984.5
59	155117.0
60	155117.0
61	155117.0
62	137911.0
63	120705.0
64	120705.0
65	120705.0
66	99881.5
67	79058.0
68	79058.0
69	79058.0
70	60240.5
71	41423.0
72	41423.0
73	41423.0
74	31988.0
75	22553.0
76	22553.0
77	22553.0
78	18166.0
79	13779.0
80	13779.0
81	13779.0
82	9711.0
83	5643.0
84	5643.0
85	5643.0
86	4145.5
87	2648.0
88	2648.0
89	2648.0
90	1779.0
91	910.0
92	910.0
93	910.0
94	561.0
95	212.0
96	212.0
97	212.0
98	469.5
99	727.0
100	727.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008507081679782268
2	0.0034773472559693945
3	0.0044087795566754824
4	0.014281961944160012
5	0.035642809373686295
6	0.046012755654880735
7	0.06762198503126197
8	0.08898283246078825
9	0.09463352175173852
10	0.11754675634910827
11	0.11767094732253575
12	0.1317045273198408
13	0.12102410360507766
14	0.12493611926804324
15	0.11406940909313888
16	0.11158558962458931
17	0.12332163661348601
18	0.13015214015199733
19	0.13102147696598967
20	0.12114829457850515
21	0.12673688838274166
22	0.149277550059829
23	0.1387834128052071
24	0.13276015059397436
25	0.13064890404570725
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1610423.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.100743564500476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70018403547986	43.364386590179706
2	13.304408855830339	14.661656412847531
3	3.795018227973147	6.273249786064601
4	1.4892820853359172	3.2824220111719558
5	0.7354572034653198	2.026211938540362
6	0.41274346264136136	1.364548301575538
7	0.2882215238163974	1.1116854191503813
8	0.189908162215704	0.8371264757642451
9	0.13526048879306024	0.6707658156655862
>10	0.7707721351989536	8.048364442045079
>50	0.0837226729295754	3.2142413455663337
>100	0.0846018455336562	9.851661585585795
>500	0.007701222320587277	2.8499629839650336
>1k	0.002718078466089627	2.443716891878054
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3786	0.23509351269821654	No Hit
GTACATGGAAGCAGTGGTATCAACG	3061	0.19007428483075567	No Hit
TCCATGTACTCTGCGTTGATACCAC	2504	0.15548709873120292	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2465	0.1530653747493671	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2274	0.1412051367870429	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1877	0.11655322856168844	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	6.209548671373918E-5	0.0	0.0	0.0	0.0
6	6.209548671373918E-5	0.0	0.0	0.0	6.209548671373918E-5
7	6.209548671373918E-5	0.0	0.0	0.0	6.209548671373918E-5
8	1.2419097342747837E-4	0.0	0.0	0.0	6.209548671373918E-5
9	1.2419097342747837E-4	0.0	0.0	6.209548671373918E-5	6.209548671373918E-5
10	1.2419097342747837E-4	0.0	0.0	1.2419097342747837E-4	1.2419097342747837E-4
11	1.2419097342747837E-4	0.0	0.0	1.8628646014121755E-4	1.2419097342747837E-4
12	1.2419097342747837E-4	0.0	0.0	2.4838194685495674E-4	3.725729202824351E-4
13	1.2419097342747837E-4	0.0	0.0	2.4838194685495674E-4	4.346684069961743E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGAAC	125	1.03682396E-10	12.915393	3
GCATCGC	210	0.0	12.667267	14
TTATACG	75	1.486463E-5	12.660184	2
AGGACGT	360	0.0	12.402209	5
CGCATCG	210	0.0	12.214865	13
ATCGCCA	220	0.0	12.093362	16
TCGTTAG	55	0.003067436	12.091483	14
AAAATCG	55	0.0030695791	12.090355	5
TATCCCG	55	0.0030695791	12.090355	5
ACACGCT	270	0.0	11.964274	9
CGCGCCA	105	2.716024E-7	11.763194	10
GCGTATG	65	8.016312E-4	11.69359	8
CGTCTTA	65	8.020494E-4	11.6928625	15
GGACGTG	375	0.0	11.654611	6
CGAGCGA	90	7.451805E-6	11.612384	10
CGGTCTG	90	7.4569634E-6	11.611663	15
ATATACG	230	0.0	11.566486	6
CGCAAGA	295	0.0	11.265418	2
TAGGACG	405	0.0	11.256993	4
ATATATC	135	4.7530193E-9	11.255245	3
>>END_MODULE