FastQCFastQC Report
Thu 2 Feb 2017
SRR4062670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610423
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC609.75576E-625.6666133
ATACCGA500.002580838521.9999526
TCTAGCG5250.021.37138428
CTAGCGG5400.021.18513929
CGCAATA5300.020.75467336
GGACCGT1700.020.705846
TAGCGGC5550.020.6125730
CGCCGGT5950.018.8571037
CGGTTGA600.007413233618.33329414
ATACGAA6000.018.33329440
AATACGA6050.018.1817839
CGAATGC6300.017.80948643
ATACCGT3350.017.7313066
TACGAAT6500.017.59996241
CAATACG6250.017.59996238
CGGTCCA6550.017.46561210
ACCGTCG3150.017.460288
TCTTGCG6750.017.2740382
GCAATAC6700.017.0745937
GATATAC3100.017.033811
Produced by FastQC (version 0.11.5)