FastQCFastQC Report
Thu 2 Feb 2017
SRR4062669_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062669_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185315
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG112570.9497053525855996No Hit
TCCATGTACTCTGCGTTGATACCAC94000.7930381375414973No Hit
GAGTACATGGAAGCAGTGGTATCAA44720.37728367564740173No Hit
GTATCAACGCAGAGTACTTTTTTTT39300.33155743409979627No Hit
CATGTACTCTGCGTTGATACCACTG38130.321686640260184No Hit
GCTTCCATGTACTCTGCGTTGATAC30990.2614494881107554No Hit
CCCATGTACTCTGCGTTGATACCAC29660.2502288421221363No Hit
GTACATGGGAAGCAGTGGTATCAAC27790.23445244513061927No Hit
CATGGAAGCAGTGGTATCAACGCAG27010.22787191590421113No Hit
CCATAGGGTCTTCTCGTCTTATTAT26260.22154448395574172No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25880.21833858510185056No Hit
TATCAACGCAGAGTACTTTTTTTTT25110.2118424216347553No Hit
GCGTTGATACCACTGCTTCCATGTA24920.21023947220780975No Hit
GTATCAACGCAGAGTACATGGAAGC23510.19834390014468725No Hit
GTATTAGAGGCACTGCCTGCCCAGT18270.15413624226471445No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17820.1503397830956328No Hit
ACTCTGCGTTGATACCACTGCTTCC16650.14046898925602055No Hit
GTCCTACAGTGGACATTTCTAAATT16530.13945660014426545No Hit
ACGCAGAGTACATGGAAGCAGTGGT16520.13937223438495253No Hit
GTATCAACGCAGAGTACATGGGAAG16140.13616633553106136No Hit
CAGTGGTATCAACGCAGAGTACATG15780.13312916819579604No Hit
CTCTAATACTTGTAATGCTAGAGGT15680.1322855106026668No Hit
GTCCTAAAGTGTGTATTTCTCATTT15500.13076692693503417No Hit
GGTATCAACGCAGAGTACTTTTTTT15410.13000763510121782No Hit
CTGTAGGACGTGGAATATGGCAAGA15370.1296701720639661No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15310.12916397750808856No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15030.12680173624732666No Hit
GGGTAGGCACACGCTGAGCCAGTCA14920.1258737128948845No Hit
CCATTGGGATGTCCTGATCCAACAT14690.12393330043068719No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14170.11954628094641509No Hit
GTCAGGATACCGCGGCCGTTAAACT14160.11946191518710217No Hit
GAATAGGACCGCGGTTCTATTTTGT13720.11574982177733346No Hit
GCGCAAGACGGACCAGAGCGAAAGC13640.11507489570283004No Hit
CTGTTAGTATGAGTAACAAGAATTC13620.1149061641842042No Hit
GTACATGGGGAATAATTGCAATCCC13450.11347194627588447No Hit
GTACATGGGGTGGTATCAACGCAAA13420.11321884899794568No Hit
CTTCTACACCATTGGGATGTCCTGA13420.11321884899794568No Hit
TATCAACGCAGAGTACATGGAAGCA13340.11254392292344229No Hit
ATCTATAACTTTATAGATGCAACAC13270.11195336260825181No Hit
CTTTAGGACGTGAAATATGGCGAGG12950.1092536583102382No Hit
GTATCTGATCGTCTTCGAACCTCCG12920.10900056103229944No Hit
GTTATATAATTTAAGCTCCATAGGG12760.10765070888329262No Hit
GATTAAGAGGGACGGCCGGGGGCAT12620.10646958825291167No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC12470.1052041018632178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA2050.015.29288710
CTGTGCG508.710154E-515.20020310
CTAGACA508.719572E-515.19827654
GGTCGGT456.759965E-414.77797411
TGCGCTA456.759965E-414.77797410
GTTTTCG1500.014.56747515
TCCAACG1600.014.25259818
CGGTCTA400.005271741714.25259816
TTTTCGG1600.014.25259816
GTCCCGA400.00527321914.25199512
CCAACGT400.00527321914.25199519
TTGTGCG400.00527765414.250197
GGTAGCG400.00527765414.250197
ATCACGC400.005286533414.2465793
AGCCCCG2200.013.82070116
CGCGGGA551.9593097E-413.81836511
GCCGCGC551.9593097E-413.8183658
TAACTTT2000.013.7751846
CGCATCG1800.013.72356413
TGCCCGG707.2580497E-613.571609510
Produced by FastQC (version 0.11.5)