##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062669_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1185315
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.908180525851776	32.0	32.0	32.0	32.0	32.0
2	30.554383433939503	32.0	32.0	32.0	32.0	32.0
3	30.56384083555848	32.0	32.0	32.0	32.0	32.0
4	30.61018632177944	32.0	32.0	32.0	32.0	32.0
5	30.44064489186419	32.0	32.0	32.0	32.0	32.0
6	34.02193762839414	36.0	36.0	36.0	32.0	36.0
7	33.83224965515496	36.0	36.0	36.0	32.0	36.0
8	33.81980486199871	36.0	36.0	36.0	32.0	36.0
9	33.996373116007135	36.0	36.0	36.0	32.0	36.0
10	33.65340015101471	36.0	36.0	36.0	27.0	36.0
11	34.03760856818651	36.0	36.0	36.0	32.0	36.0
12	33.769943854587176	36.0	36.0	36.0	32.0	36.0
13	33.91946950810544	36.0	36.0	36.0	32.0	36.0
14	33.79901882621919	36.0	36.0	36.0	32.0	36.0
15	33.76551802685362	36.0	36.0	36.0	32.0	36.0
16	33.7565204186229	36.0	36.0	36.0	32.0	36.0
17	33.65681865158207	36.0	36.0	36.0	27.0	36.0
18	33.70131230938611	36.0	36.0	36.0	27.0	36.0
19	33.66519279685147	36.0	36.0	36.0	27.0	36.0
20	33.629436057081875	36.0	36.0	36.0	27.0	36.0
21	33.57093683957429	36.0	36.0	36.0	27.0	36.0
22	33.519768162893406	36.0	36.0	36.0	27.0	36.0
23	33.4675103242598	36.0	36.0	36.0	27.0	36.0
24	33.45291420424107	36.0	36.0	36.0	27.0	36.0
25	32.94764007879762	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	6.0
4	67.0
5	117.0
6	209.0
7	59.0
8	165.0
9	212.0
10	131.0
11	51.0
12	79.0
13	68.0
14	423.0
15	456.0
16	663.0
17	944.0
18	1347.0
19	1906.0
20	2970.0
21	4278.0
22	6082.0
23	8562.0
24	10925.0
25	14338.0
26	18788.0
27	22881.0
28	29108.0
29	37353.0
30	47024.0
31	62314.0
32	87106.0
33	121330.0
34	254681.0
35	450671.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30081344028458	19.203130576529283	12.43261759700577	24.063438386180376
2	14.51674002529375	21.353561584546345	39.935525402164735	24.19417298799517
3	18.60806324377455	26.870420289304832	28.941620157687588	25.579896309233035
4	11.095088584695272	17.50126778591842	38.04041220550873	33.36323142387758
5	12.251143375343604	39.32021177024633	34.39013089210706	14.038513962303007
6	30.880646209553873	37.46740887356458	18.649847436811832	13.002097480069718
7	28.157756105467907	32.78835503566025	21.41565471687919	17.63823414199265
8	27.061086557444604	34.305731709582574	20.415130537874475	18.218051195098347
9	26.886497679351972	16.141762206203257	19.969902512515286	37.00183760192948
10	15.865992948597743	27.11664546515773	32.2597429802733	24.757618605971224
11	34.50795058599937	23.09027557808046	23.445887445887443	18.95588639003273
12	24.033781837320365	26.10235758160973	29.332110063841487	20.531750517228417
13	28.21481394510044	21.203661547584154	26.6140483211431	23.967476186172313
14	22.94786867575928	22.535136313183276	25.557898258009253	28.959096753048186
15	24.297631928132645	28.89652428842553	24.80288591074522	22.002957872696605
16	24.350130788897346	27.185424618381056	26.543515919758033	21.920928672963566
17	21.861405307874637	27.67399938842364	27.43578870543475	23.028806598266975
18	23.1738522557836	25.468453665727548	30.09896640717728	21.25872767131157
19	23.50618477098353	28.16110986268643	26.76144744423062	21.571257922099427
20	23.391460655934342	25.839599674955526	28.667351445045693	22.101588224064443
21	24.945279692366697	26.248091864224364	24.93370627338443	23.87292217002451
22	23.203369050063415	28.03108462423076	25.967761151655417	22.797785174050407
23	21.727566666976454	27.47550496068374	26.713089733332097	24.083838639007713
24	22.335117581262693	28.69504904992177	26.498193786897268	22.471639581918268
25	23.473845251883223	26.87906074206986	26.438509034477924	23.208584971569
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	594.0
1	594.0
2	976.0
3	1358.0
4	1358.0
5	1358.0
6	3264.5
7	5171.0
8	5171.0
9	5171.0
10	5098.0
11	5025.0
12	5025.0
13	5025.0
14	5588.5
15	6152.0
16	6152.0
17	6152.0
18	9984.0
19	13816.0
20	13816.0
21	13816.0
22	21698.5
23	29581.0
24	29581.0
25	29581.0
26	44938.0
27	60295.0
28	60295.0
29	60295.0
30	72130.0
31	83965.0
32	83965.0
33	83965.0
34	92394.5
35	100824.0
36	100824.0
37	100824.0
38	103618.5
39	106413.0
40	106413.0
41	106413.0
42	114997.5
43	123582.0
44	123582.0
45	123582.0
46	158039.5
47	192497.0
48	192497.0
49	192497.0
50	167042.0
51	141587.0
52	141587.0
53	141587.0
54	117534.0
55	93481.0
56	93481.0
57	93481.0
58	83603.0
59	73725.0
60	73725.0
61	73725.0
62	65979.5
63	58234.0
64	58234.0
65	58234.0
66	49617.0
67	41000.0
68	41000.0
69	41000.0
70	30959.5
71	20919.0
72	20919.0
73	20919.0
74	17078.5
75	13238.0
76	13238.0
77	13238.0
78	10608.5
79	7979.0
80	7979.0
81	7979.0
82	5541.0
83	3103.0
84	3103.0
85	3103.0
86	2318.0
87	1533.0
88	1533.0
89	1533.0
90	1035.0
91	537.0
92	537.0
93	537.0
94	324.0
95	111.0
96	111.0
97	111.0
98	353.0
99	595.0
100	595.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008605307449918376
2	0.003543361891142861
3	0.005061945558775515
4	0.015017105157700696
5	0.03762712865356466
6	0.047244825215238144
7	0.06774570472827898
8	0.0895120706310137
9	0.09811737808093207
10	0.12123359613267361
11	0.12148669341061236
12	0.13515394641930625
13	0.12182415644786405
14	0.1262955416914491
15	0.11439996962832664
16	0.11355631203519738
17	0.1256206156169457
18	0.1332978997144219
19	0.13506958065999333
20	0.12460822650519061
21	0.1322855106026668
22	0.15489553409853077
23	0.14409671690647632
24	0.1369256273648777
25	0.1326229736399185
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1185315.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.85062987817787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.26376053947459	27.69954482096868
2	13.017060876817995	9.33339663193023
3	4.010940568375062	4.313842373405482
4	1.7132275692695325	2.4568114993188934
5	0.8775087323489448	1.572962038915553
6	0.5339334115280266	1.1485109469770465
7	0.3387559000895986	0.850122867521284
8	0.2436913508927697	0.6989190740295885
9	0.18381482939692276	0.5930889673346538
>10	1.311910778804788	9.752778621945543
>50	0.2224978428729642	5.681030861715093
>100	0.2357086760112324	17.656145738794574
>500	0.03279630226168656	8.140629178060346
>1k	0.01392073261467271	8.354889651548502
>5k	2.3594462058767308E-4	0.7951237468569627
>10k+	2.3594462058767308E-4	0.9522029806775351
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	11257	0.9497053525855996	No Hit
TCCATGTACTCTGCGTTGATACCAC	9400	0.7930381375414973	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4472	0.37728367564740173	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3930	0.33155743409979627	No Hit
CATGTACTCTGCGTTGATACCACTG	3813	0.321686640260184	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3099	0.2614494881107554	No Hit
CCCATGTACTCTGCGTTGATACCAC	2966	0.2502288421221363	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2779	0.23445244513061927	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2701	0.22787191590421113	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	2626	0.22154448395574172	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2588	0.21833858510185056	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2511	0.2118424216347553	No Hit
GCGTTGATACCACTGCTTCCATGTA	2492	0.21023947220780975	No Hit
GTATCAACGCAGAGTACATGGAAGC	2351	0.19834390014468725	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1827	0.15413624226471445	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1782	0.1503397830956328	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1665	0.14046898925602055	No Hit
GTCCTACAGTGGACATTTCTAAATT	1653	0.13945660014426545	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1652	0.13937223438495253	No Hit
GTATCAACGCAGAGTACATGGGAAG	1614	0.13616633553106136	No Hit
CAGTGGTATCAACGCAGAGTACATG	1578	0.13312916819579604	No Hit
CTCTAATACTTGTAATGCTAGAGGT	1568	0.1322855106026668	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1550	0.13076692693503417	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1541	0.13000763510121782	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1537	0.1296701720639661	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1531	0.12916397750808856	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1503	0.12680173624732666	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1492	0.1258737128948845	No Hit
CCATTGGGATGTCCTGATCCAACAT	1469	0.12393330043068719	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1417	0.11954628094641509	No Hit
GTCAGGATACCGCGGCCGTTAAACT	1416	0.11946191518710217	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1372	0.11574982177733346	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1364	0.11507489570283004	No Hit
CTGTTAGTATGAGTAACAAGAATTC	1362	0.1149061641842042	No Hit
GTACATGGGGAATAATTGCAATCCC	1345	0.11347194627588447	No Hit
GTACATGGGGTGGTATCAACGCAAA	1342	0.11321884899794568	No Hit
CTTCTACACCATTGGGATGTCCTGA	1342	0.11321884899794568	No Hit
TATCAACGCAGAGTACATGGAAGCA	1334	0.11254392292344229	No Hit
ATCTATAACTTTATAGATGCAACAC	1327	0.11195336260825181	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1295	0.1092536583102382	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1292	0.10900056103229944	No Hit
GTTATATAATTTAAGCTCCATAGGG	1276	0.10765070888329262	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1262	0.10646958825291167	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1247	0.1052041018632178	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.5309727793877576E-4	0.0
8	0.0	0.0	0.0	3.3746303725170105E-4	0.0
9	0.0	0.0	0.0	4.218287965646263E-4	0.0
10	0.0	0.0	0.0	4.218287965646263E-4	0.0
11	0.0	0.0	0.0	4.218287965646263E-4	0.0
12	0.0	0.0	0.0	4.218287965646263E-4	0.0
13	0.0	0.0	0.0	4.218287965646263E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCCA	205	0.0	15.292887	10
CTGTGCG	50	8.710154E-5	15.200203	10
CTAGACA	50	8.719572E-5	15.1982765	4
GGTCGGT	45	6.759965E-4	14.777974	11
TGCGCTA	45	6.759965E-4	14.777974	10
GTTTTCG	150	0.0	14.567475	15
TCCAACG	160	0.0	14.252598	18
CGGTCTA	40	0.0052717417	14.252598	16
TTTTCGG	160	0.0	14.252598	16
GTCCCGA	40	0.005273219	14.251995	12
CCAACGT	40	0.005273219	14.251995	19
TTGTGCG	40	0.005277654	14.25019	7
GGTAGCG	40	0.005277654	14.25019	7
ATCACGC	40	0.0052865334	14.246579	3
AGCCCCG	220	0.0	13.820701	16
CGCGGGA	55	1.9593097E-4	13.818365	11
GCCGCGC	55	1.9593097E-4	13.818365	8
TAACTTT	200	0.0	13.775184	6
CGCATCG	180	0.0	13.723564	13
TGCCCGG	70	7.2580497E-6	13.5716095	10
>>END_MODULE