##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062669_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1185315
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.180409427029947	32.0	32.0	32.0	32.0	32.0
2	31.386908965127414	32.0	32.0	32.0	32.0	32.0
3	31.48906661942184	32.0	32.0	32.0	32.0	32.0
4	31.59750614815471	32.0	32.0	32.0	32.0	32.0
5	31.542298038917924	32.0	32.0	32.0	32.0	32.0
6	35.09621577386602	36.0	36.0	36.0	36.0	36.0
7	35.10970585878015	36.0	36.0	36.0	36.0	36.0
8	35.060232933861464	36.0	36.0	36.0	36.0	36.0
9	35.17066265085652	36.0	36.0	36.0	36.0	36.0
10	35.07532512454495	36.0	36.0	36.0	36.0	36.0
11	35.185598764885285	36.0	36.0	36.0	36.0	36.0
12	35.09469212825283	36.0	36.0	36.0	36.0	36.0
13	35.14605400252253	36.0	36.0	36.0	36.0	36.0
14	35.09961571396633	36.0	36.0	36.0	36.0	36.0
15	35.075012971235495	36.0	36.0	36.0	36.0	36.0
16	35.07102247081999	36.0	36.0	36.0	36.0	36.0
17	35.045553291741015	36.0	36.0	36.0	36.0	36.0
18	35.057672433066315	36.0	36.0	36.0	36.0	36.0
19	35.029312039415686	36.0	36.0	36.0	36.0	36.0
20	35.00411704905447	36.0	36.0	36.0	36.0	36.0
21	34.97498386504853	36.0	36.0	36.0	36.0	36.0
22	34.94744856852398	36.0	36.0	36.0	36.0	36.0
23	34.8817284856768	36.0	36.0	36.0	32.0	36.0
24	34.852973260272584	36.0	36.0	36.0	32.0	36.0
25	34.82449559821651	36.0	36.0	36.0	32.0	36.0
26	34.77466243150555	36.0	36.0	36.0	32.0	36.0
27	34.73264828336771	36.0	36.0	36.0	32.0	36.0
28	34.68618890337168	36.0	36.0	36.0	32.0	36.0
29	34.65127919582558	36.0	36.0	36.0	32.0	36.0
30	34.616725511783784	36.0	36.0	36.0	32.0	36.0
31	34.59930229517048	36.0	36.0	36.0	32.0	36.0
32	34.55026385391225	36.0	36.0	36.0	32.0	36.0
33	34.512778459734335	36.0	36.0	36.0	32.0	36.0
34	34.480200621775644	36.0	36.0	36.0	32.0	36.0
35	34.398824784972774	36.0	36.0	36.0	32.0	36.0
36	34.345372327187285	36.0	36.0	36.0	32.0	36.0
37	34.28543045519545	36.0	36.0	36.0	32.0	36.0
38	34.215354568194954	36.0	36.0	36.0	32.0	36.0
39	34.0760067998802	36.0	36.0	36.0	32.0	36.0
40	34.00329617021636	36.0	36.0	36.0	32.0	36.0
41	33.985346511264936	36.0	36.0	36.0	32.0	36.0
42	33.853827885414425	36.0	36.0	36.0	32.0	36.0
43	33.8757494843143	36.0	36.0	36.0	32.0	36.0
44	33.8481585063886	36.0	36.0	36.0	32.0	36.0
45	33.65307871747173	36.0	36.0	36.0	27.0	36.0
46	33.716264452909144	36.0	36.0	36.0	27.0	36.0
47	33.63274319484694	36.0	36.0	36.0	21.0	36.0
48	33.59531263841257	36.0	36.0	36.0	21.0	36.0
49	33.61625306353164	36.0	36.0	36.0	21.0	36.0
50	33.05970396055057	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	13.0
21	52.0
22	206.0
23	538.0
24	1363.0
25	3094.0
26	6245.0
27	11491.0
28	18617.0
29	26857.0
30	36294.0
31	48817.0
32	70230.0
33	98021.0
34	198168.0
35	665305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.97992552759723	19.72582072789897	12.953861030412517	24.340392714091283
2	14.136163642729684	21.73615183102513	39.460964425676956	24.666720100568227
3	18.251052259035	26.76825497529332	29.403437252964842	25.577255512706838
4	10.887531531835554	17.729623474027893	38.273025621989184	33.10981937214737
5	12.123612710545299	39.21472351231529	34.465353091794164	14.196310685345246
6	31.09838869020652	37.65878770852157	18.24081315505071	13.002010446221195
7	28.03586222722418	32.68686609624116	21.522820113488915	17.75445156304574
8	26.96380287096679	34.390351931764975	20.384960959744877	18.26088423752336
9	26.81295689331528	15.70038344237608	20.05795927664798	37.42870038766066
10	15.914925568308846	27.024040023116218	32.37628815968751	24.684746248887425
11	34.718281638214314	22.790988049590194	23.561416163635826	18.929314148559666
12	23.900144687277223	26.07981844488596	29.428548529293902	20.591488338542916
13	28.468297456794183	20.990285282815115	26.703112674689848	23.838304585700847
14	22.824481256037423	22.313393486119722	25.835410840156413	29.02671441768644
15	24.4809185743874	28.67811510020543	24.781682506337976	22.059283819069194
16	24.4215250798311	26.99603059102433	26.47726553700915	22.105178792135423
17	21.849972370213823	27.38326942627065	27.7047029692529	23.062055234262623
18	23.358600878247554	24.950245293445203	30.401201368412618	21.289952459894625
19	23.645022631114937	27.700231584009316	27.126966249477984	21.527779535397766
20	23.508518832546624	25.45146226952329	29.021821203646287	22.0181976942838
21	24.951510779834894	25.880968350185395	25.380679397459748	23.786841472519964
22	23.276934823232644	27.502731341457753	26.485786478699758	22.734547356609845
23	21.740043785829084	26.934781049763146	27.18770959618329	24.13746556822448
24	22.4007120470086	28.10974297971425	26.947267182141456	22.54227779113569
25	23.548339470942324	26.135415480273178	26.933093734576886	23.383151314207616
26	20.91199385817272	27.07086301953489	29.06746307943458	22.949680042857807
27	23.65674947165943	27.4664540649532	27.00075507354585	21.876041389841518
28	21.90607560015692	26.13727152697806	28.128725275559663	23.827927597305358
29	21.81614170072934	26.690795273830165	28.563462033299164	22.92960099214133
30	23.231461678962976	25.965840304054193	28.84414691453327	21.95855110244956
31	22.734969185406413	25.86519195319388	27.361165597330668	24.038673264069043
32	21.556042064767595	27.374917216098677	27.735412105642805	23.333628613490927
33	21.442738850010336	26.86695097927555	28.07388753200626	23.616422638707853
34	21.730594820786038	27.140127307930804	29.454701914680907	21.674575956602254
35	24.075288003610854	26.7106212272687	27.480796244036394	21.733294525084048
36	21.897217195429064	27.15843467770171	27.894104098910415	23.050244027958815
37	24.24663486077541	26.79363713443262	26.483677334716933	22.476050670075043
38	22.320564575661322	27.29493847627002	26.832107920679316	23.552389027389346
39	22.658365075950275	26.93790258285772	26.838604084146407	23.565128257045593
40	23.877534663781358	26.944061283287564	27.121229377844706	22.05717467508637
41	22.077253725802844	26.812872527555964	28.534187114817577	22.575686631823608
42	24.23001480619076	28.051277508510395	26.89571970320126	20.822987982097587
43	22.545230592711643	26.515651957496527	26.957391073259007	23.98172637653282
44	22.202030683826663	26.732809421968	28.201279828568776	22.86388006563656
45	22.97490540489237	27.1342217047789	27.423849356500167	22.46702353382856
46	22.850335734105677	26.106943904268327	27.784053663462732	23.258666698163267
47	24.037998337994544	25.831108186431457	27.780041592319343	22.350851883254663
48	22.82203464901735	29.17764476109726	25.42665873628529	22.573661853600097
49	21.87781307078709	26.859526792456013	28.533512188743078	22.72914794801382
50	21.147307801983274	29.336024040887054	25.63253281408977	23.8841353430399
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	59.0
1	51.0
2	43.0
3	241.0
4	439.0
5	418.5
6	398.0
7	375.0
8	352.0
9	468.5
10	585.0
11	866.5
12	1148.0
13	2116.0
14	3084.0
15	4147.0
16	5210.0
17	6294.5
18	7379.0
19	7596.5
20	7814.0
21	8840.5
22	9867.0
23	10133.5
24	10400.0
25	13041.5
26	15683.0
27	20783.0
28	25883.0
29	29278.0
30	32673.0
31	32986.5
32	33300.0
33	38575.5
34	43851.0
35	50394.0
36	56937.0
37	65762.0
38	74587.0
39	72540.5
40	70494.0
41	72591.0
42	74688.0
43	69775.5
44	64863.0
45	73423.5
46	81984.0
47	88092.5
48	94201.0
49	89215.0
50	84229.0
51	72563.5
52	60898.0
53	56184.0
54	51470.0
55	52254.0
56	53038.0
57	53070.0
58	53102.0
59	47908.0
60	42714.0
61	37437.0
62	32160.0
63	28537.5
64	24915.0
65	21717.0
66	18519.0
67	17036.0
68	15553.0
69	14664.5
70	13776.0
71	10287.0
72	6798.0
73	5893.0
74	4988.0
75	3561.5
76	2135.0
77	1960.0
78	1785.0
79	1538.0
80	1291.0
81	991.5
82	692.0
83	657.0
84	622.0
85	519.5
86	417.0
87	305.5
88	194.0
89	121.5
90	49.0
91	36.5
92	24.0
93	17.0
94	10.0
95	6.5
96	3.0
97	4.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015017105157700696
2	0.004218287965646263
3	6.74926074503402E-4
4	4.218287965646263E-4
5	0.0
6	5.061945558775515E-4
7	8.436575931292525E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.687315186258505E-4
47	0.0
48	0.0
49	0.0
50	8.436575931292525E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1185315.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.01270438233227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.45418299163521	31.76605510211002
2	13.102963399900561	10.747759289052821
3	4.00307247088304	4.925304836081355
4	1.6809504494536485	2.7576129545916417
5	0.8440102900031051	1.730757225977194
6	0.5206846221429724	1.2812810690625662
7	0.3331324203583232	0.9563863033428729
8	0.24717185394935712	0.8109748942126391
9	0.18972870950014253	0.7003158728013668
>10	1.2047405079034847	9.88979147478771
>50	0.18953444423297594	5.4455080034524554
>100	0.19717244561007954	16.781593151313814
>500	0.023768166829880963	6.728047512936465
>1k	0.008680547885695656	4.999351344480866
>5k	2.0667971156418228E-4	0.47926096579621247
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5654	0.4770040031552794	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	4542	0.38318927879930653	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	2476	0.20888962005880296	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1980	0.167044203439592	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	1905	0.16071677149112262	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1752	0.14780881031624504	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1519	0.12815158839633345	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1507	0.12713919928457837	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	1501	0.12663300472870082	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1466	0.12368020315274844	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1464	0.12351147163412257	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	1450	0.12233035100374162	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1420	0.11979937822435387	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1416	0.11946191518710217	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	1383	0.11667784512977564	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	1378	0.116256016333211	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1362	0.1149061641842042	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1355	0.11431560386901372	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	1352	0.11406250659107495	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1295	0.1092536583102382	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	1274	0.10748197736466679	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	1258	0.10613212521565997	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1249	0.10537283338184365	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1239	0.1045291757887144	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1229	0.10368551819558514	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1224	0.10326368939902052	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	1220	0.10292622636176882	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1218	0.10275749484314296	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.436575931292526E-5	0.0
10	0.0	0.0	0.0	8.436575931292526E-5	0.0
11	0.0	0.0	0.0	8.436575931292526E-5	0.0
12	0.0	0.0	0.0	1.6873151862585053E-4	0.0
13	0.0	0.0	0.0	1.6873151862585053E-4	0.0
14	0.0	0.0	0.0	1.6873151862585053E-4	0.0
15	0.0	0.0	0.0	2.5309727793877576E-4	0.0
16	0.0	0.0	0.0	4.218287965646263E-4	0.0
17	0.0	0.0	0.0	4.218287965646263E-4	0.0
18	0.0	0.0	0.0	6.749260745034021E-4	0.0
19	0.0	0.0	0.0	6.749260745034021E-4	0.0
20	0.0	0.0	0.0	9.280233524421778E-4	0.0
21	0.0	0.0	0.0	0.0015185836676326547	0.0
22	0.0	0.0	0.0	0.0026997042980136084	0.0
23	0.0	0.0	0.0	0.005230677077401366	0.0
24	0.0	0.0	0.0	0.007930381375414974	0.0
25	0.0	0.0	0.0	0.00995515959892518	0.0
26	0.0	0.0	0.0	0.012992326934190489	0.0
27	0.0	0.0	0.0	0.0163669573067075	0.0
28	0.0	0.0	0.0	0.02345368108899322	0.0
29	0.0	0.0	0.0	0.03324010916929255	0.0
30	0.0	0.0	0.0	0.048172848567680324	0.0
31	0.0	0.0	0.0	0.08824658424131981	0.0
32	0.0	0.0	0.0	0.13304480243648312	0.0
33	0.0	0.0	0.0	0.1677191295140954	0.0
34	0.0	0.0	0.0	0.21555451504452403	0.0
35	0.0	0.0	0.0	0.27039225859792543	0.0
36	0.0	0.0	0.0	0.3504553641858915	0.0
37	0.0	0.0	0.0	0.4625774583127692	0.0
38	0.0	0.0	0.0	0.5989125253624564	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGCCG	30	0.0057450766	29.33322	5
TAGCGAG	40	7.0322066E-4	27.499895	18
TCGTATA	50	8.3415136E-5	26.399899	44
TGGTATA	380	0.0	22.578861	44
AGACCGT	60	2.8728065E-4	21.999916	6
GATATAC	440	0.0	21.503546	1
GTTATAT	295	0.0	20.8848	1
GCGAGAA	815	0.0	20.515259	20
TGTAGGA	1215	0.0	20.460827	2
ATAGCGA	55	0.004483661	19.999924	17
GTCCGAC	55	0.004483661	19.999924	12
AGGACCT	895	0.0	19.910536	5
GGCGAGA	850	0.0	19.670513	19
TAGGACG	1225	0.0	19.575436	4
GTCGTAT	90	1.0019661E-5	19.555481	43
TAGGACC	1060	0.0	19.509357	4
TTAGGAC	715	0.0	19.38454	3
ACGCTTA	125	2.2879249E-8	19.359924	17
CGCTTAC	125	2.2879249E-8	19.359924	18
CTGTAGG	1160	0.0	19.348019	1
>>END_MODULE