FastQCFastQC Report
Thu 2 Feb 2017
SRR4062668_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062668_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082543
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG107980.5185006984249545No Hit
TCCATGTACTCTGCGTTGATACCAC92290.4431601172220694No Hit
GAGTACATGGAAGCAGTGGTATCAA50680.24335631965342375No Hit
GTATCAACGCAGAGTACTTTTTTTT44150.21200042448103112No Hit
CATGTACTCTGCGTTGATACCACTG40830.1960583767057871No Hit
TATCAACGCAGAGTACTTTTTTTTT32390.15553100224101013No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32120.15423451040386682No Hit
GCTTCCATGTACTCTGCGTTGATAC31260.15010494381148431No Hit
CCCATGTACTCTGCGTTGATACCAC28020.13454704176576426No Hit
GTACATGGGAAGCAGTGGTATCAAC26600.1277284550667141No Hit
CATGGAAGCAGTGGTATCAACGCAG25260.12129401409718792No Hit
GCGTTGATACCACTGCTTCCATGTA25030.12018959512480655No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24660.11841292112575827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAGA3500.014.3811112
CAAGACG3700.014.3758514
AAGACGG3800.013.99854855
ACGCGCG1153.765308E-1013.21792613
GCGCAAG3800.012.994281
CGCGCTA1251.0186341E-1012.92207616
TCGGCGT1900.012.50050513
AACCGCG1750.012.486527
AACGGAC1001.4416582E-712.35109515
CGGTCGG2000.012.350510
ACGGTAT2900.012.1214729
CGACCAT3450.012.11643310
AGAACCG1900.011.9987555
CGGTCCA3300.011.80350810
ATCGCCA2350.011.72528716
AGGGGCG1552.5465852E-1111.6439525
CGAACGA1001.9266936E-611.40183216
CGCGGTC1751.8189894E-1211.40046210
CGCATCG2500.011.40046213
CCGACCA3600.011.3479559
Produced by FastQC (version 0.11.5)