##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062668_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2082543
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12073508206073	32.0	32.0	32.0	32.0	32.0
2	30.731496540527615	32.0	32.0	32.0	32.0	32.0
3	30.735711579544816	32.0	32.0	32.0	32.0	32.0
4	30.812202197025464	32.0	32.0	32.0	32.0	32.0
5	30.573563186930595	32.0	32.0	32.0	32.0	32.0
6	34.23354907917868	36.0	36.0	36.0	32.0	36.0
7	34.09611518225554	36.0	36.0	36.0	32.0	36.0
8	34.0813188491186	36.0	36.0	36.0	32.0	36.0
9	34.26792820124243	36.0	36.0	36.0	32.0	36.0
10	33.89645928079276	36.0	36.0	36.0	32.0	36.0
11	34.28840028753308	36.0	36.0	36.0	32.0	36.0
12	34.0214156442388	36.0	36.0	36.0	32.0	36.0
13	34.15626279985575	36.0	36.0	36.0	32.0	36.0
14	34.05125512414389	36.0	36.0	36.0	32.0	36.0
15	33.988373829495956	36.0	36.0	36.0	32.0	36.0
16	33.980333659377024	36.0	36.0	36.0	32.0	36.0
17	33.895953648976274	36.0	36.0	36.0	32.0	36.0
18	33.9145731924863	36.0	36.0	36.0	32.0	36.0
19	33.90213695467513	36.0	36.0	36.0	32.0	36.0
20	33.89336546712361	36.0	36.0	36.0	32.0	36.0
21	33.85124436806347	36.0	36.0	36.0	32.0	36.0
22	33.80202377573956	36.0	36.0	36.0	32.0	36.0
23	33.74663428318167	36.0	36.0	36.0	32.0	36.0
24	33.71171927782524	36.0	36.0	36.0	27.0	36.0
25	33.19733181979916	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	61.0
5	189.0
6	355.0
7	112.0
8	314.0
9	336.0
10	233.0
11	93.0
12	158.0
13	103.0
14	401.0
15	498.0
16	768.0
17	1056.0
18	1486.0
19	2157.0
20	3185.0
21	4556.0
22	6932.0
23	10066.0
24	13916.0
25	19245.0
26	26667.0
27	34152.0
28	45817.0
29	61441.0
30	79657.0
31	109421.0
32	156272.0
33	222742.0
34	475207.0
35	804937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.05783563454195	17.2011021982497	11.12254031917264	25.618521848035712
2	16.1557111719683	20.154614397991615	37.56398048280144	26.12569394723864
3	19.27475023469895	24.42367302054546	28.12257647824323	28.17900026651236
4	12.351190547655284	15.43200064160532	36.20003486567829	36.016773945061104
5	14.114499398370196	37.0241300384995	33.94484219526429	14.916528367866002
6	34.005517947742355	35.13053362100067	17.317929582992843	13.546018848264124
7	29.870332677933398	30.760840526395704	21.09359533954906	18.27523145612184
8	28.1084120503757	32.93279154541719	19.964676453131418	18.994119951075685
9	27.14843045943505	14.720916361182498	18.65410412592276	39.47654905345969
10	15.333399033249767	27.273269187780773	32.43507125636668	24.958260522602775
11	36.94314977777671	21.550106604682913	21.83268713508628	19.674056482454095
12	23.923199972305603	24.22437655149932	29.353321380508046	22.49910209568703
13	29.58854651176766	19.84913932978056	25.35887099681981	25.203443161631967
14	23.223008107567576	19.780272611190597	25.68224911924432	31.31447016199751
15	25.127966496044674	27.180429875129313	23.29826673178761	24.393336897038406
16	25.614728486906497	26.12721053248908	24.377195630562984	23.880865350041436
17	23.609359014791966	26.255596024707124	26.054975654407976	24.080069306092934
18	24.736273505325492	24.99816097016156	27.105905521464724	23.15966000304822
19	24.724993905848606	26.51131348555169	25.59990730158669	23.16378530701301
20	24.64212983706427	24.614534797633173	26.69622957534407	24.047105789958483
21	25.78232237735038	25.151627771909524	24.67012095124401	24.395928899496088
22	24.505076190952494	25.506849683964315	25.46539765098735	24.522676474095846
23	23.433352823061476	25.14362455090281	25.686387835332315	25.7366347907034
24	23.503903445908296	26.481359765634576	25.58926719456263	24.425469593894498
25	24.425098214942516	25.27649471579329	25.246973739312466	25.05143332995172
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	302.0
1	302.0
2	772.0
3	1242.0
4	1242.0
5	1242.0
6	3346.5
7	5451.0
8	5451.0
9	5451.0
10	5792.5
11	6134.0
12	6134.0
13	6134.0
14	6573.0
15	7012.0
16	7012.0
17	7012.0
18	11334.0
19	15656.0
20	15656.0
21	15656.0
22	24491.5
23	33327.0
24	33327.0
25	33327.0
26	48589.5
27	63852.0
28	63852.0
29	63852.0
30	78745.5
31	93639.0
32	93639.0
33	93639.0
34	114949.5
35	136260.0
36	136260.0
37	136260.0
38	154894.0
39	173528.0
40	173528.0
41	173528.0
42	200049.0
43	226570.0
44	226570.0
45	226570.0
46	270368.0
47	314166.0
48	314166.0
49	314166.0
50	297171.5
51	280177.0
52	280177.0
53	280177.0
54	251801.5
55	223426.0
56	223426.0
57	223426.0
58	202679.5
59	181933.0
60	181933.0
61	181933.0
62	158859.5
63	135786.0
64	135786.0
65	135786.0
66	111751.5
67	87717.0
68	87717.0
69	87717.0
70	66014.5
71	44312.0
72	44312.0
73	44312.0
74	34546.0
75	24780.0
76	24780.0
77	24780.0
78	20294.5
79	15809.0
80	15809.0
81	15809.0
82	11089.5
83	6370.0
84	6370.0
85	6370.0
86	4605.5
87	2841.0
88	2841.0
89	2841.0
90	1922.5
91	1004.0
92	1004.0
93	1004.0
94	606.5
95	209.0
96	209.0
97	209.0
98	624.5
99	1040.0
100	1040.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00763489637428855
2	0.0034573115657155697
3	0.004225603024763474
4	0.01277284550667141
5	0.033516714900964834
6	0.046673706137160195
7	0.06583297439716732
8	0.08537638838669838
9	0.09440381303051125
10	0.11418731810099479
11	0.1165882289105195
12	0.12964918371433387
13	0.1189891397200442
14	0.1205737408543305
15	0.1109700976162317
16	0.1092414418333739
17	0.11980544939528259
18	0.12686412717528522
19	0.12868881939052398
20	0.1180767936124248
21	0.1255196171219514
22	0.1455432132733874
23	0.1351712785762407
24	0.1303214387410008
25	0.12801656436385708
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2082543.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.239871239058466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8589130867359	37.609021545734805
2	14.562036400861961	14.631896675155746
3	4.820154055629284	7.264917573217219
4	2.1177169088997148	4.255752992955942
5	1.0917956143468714	2.742583554207777
6	0.6318566747350174	1.9046638788136148
7	0.4106916963559204	1.4443168560710355
8	0.26666578789500656	1.0717803878165761
9	0.18756764172896226	0.8481036752169222
>10	0.8829508493647791	7.9537472172831
>50	0.07581029132606253	2.6615164007465553
>100	0.07785199828239003	8.341549934444261
>500	0.010723157544110951	3.7067513789170623
>1k	0.0049786088597657974	4.356305901520888
>5k	1.9148495614483837E-4	0.6876985344836267
>10k+	9.574247807241918E-5	0.5193934934149962
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	10798	0.5185006984249545	No Hit
TCCATGTACTCTGCGTTGATACCAC	9229	0.4431601172220694	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5068	0.24335631965342375	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4415	0.21200042448103112	No Hit
CATGTACTCTGCGTTGATACCACTG	4083	0.1960583767057871	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3239	0.15553100224101013	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3212	0.15423451040386682	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3126	0.15010494381148431	No Hit
CCCATGTACTCTGCGTTGATACCAC	2802	0.13454704176576426	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2660	0.1277284550667141	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2526	0.12129401409718792	No Hit
GCGTTGATACCACTGCTTCCATGTA	2503	0.12018959512480655	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2466	0.11841292112575827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.801821619049403E-5
2	0.0	0.0	0.0	0.0	4.801821619049403E-5
3	0.0	0.0	0.0	0.0	4.801821619049403E-5
4	0.0	0.0	0.0	0.0	4.801821619049403E-5
5	0.0	0.0	0.0	0.0	4.801821619049403E-5
6	0.0	0.0	0.0	0.0	4.801821619049403E-5
7	0.0	0.0	0.0	0.0	4.801821619049403E-5
8	0.0	0.0	0.0	0.0	9.603643238098806E-5
9	0.0	0.0	0.0	4.801821619049403E-5	1.920728647619761E-4
10	0.0	0.0	0.0	4.801821619049403E-5	1.920728647619761E-4
11	0.0	0.0	0.0	1.920728647619761E-4	1.920728647619761E-4
12	0.0	0.0	0.0	1.920728647619761E-4	2.8810929714296414E-4
13	0.0	0.0	0.0	1.920728647619761E-4	2.8810929714296414E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAAGA	350	0.0	14.381111	2
CAAGACG	370	0.0	14.375851	4
AAGACGG	380	0.0	13.9985485	5
ACGCGCG	115	3.765308E-10	13.217926	13
GCGCAAG	380	0.0	12.99428	1
CGCGCTA	125	1.0186341E-10	12.922076	16
TCGGCGT	190	0.0	12.500505	13
AACCGCG	175	0.0	12.48652	7
AACGGAC	100	1.4416582E-7	12.351095	15
CGGTCGG	200	0.0	12.3505	10
ACGGTAT	290	0.0	12.121472	9
CGACCAT	345	0.0	12.116433	10
AGAACCG	190	0.0	11.998755	5
CGGTCCA	330	0.0	11.803508	10
ATCGCCA	235	0.0	11.725287	16
AGGGGCG	155	2.5465852E-11	11.643952	5
CGAACGA	100	1.9266936E-6	11.401832	16
CGCGGTC	175	1.8189894E-12	11.400462	10
CGCATCG	250	0.0	11.400462	13
CCGACCA	360	0.0	11.347955	9
>>END_MODULE