FastQCFastQC Report
Thu 2 Feb 2017
SRR4062668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082543
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA39190.1881833892505461No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC36770.17656298093244654No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC21080.1012223997295614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG801.3087083E-724.7540261
CGCAATA5550.024.18004836
TATAACG602.8724497E-422.0014672
ATACGAA6200.021.29020740
AATACGA6450.021.14717139
CGGTCCA6700.021.0148110
CAATACG6550.020.48843838
CGAATGC6500.019.96912243
CTAGCGG6500.019.96912229
TACGAAT6850.019.912341
TCTAGCG6550.019.81668728
GCAATAC7050.019.65946837
TAGCGGC6750.019.22952530
CGCCGGT6850.018.3064697
GCGCAAT7550.017.77473635
CCGGTCC7800.017.7691359
TAGGACG3100.017.7418384
TCGTATA750.001291342517.59990544
GGCGTTA4250.017.0822642
CGGCGCA9950.017.02503433
Produced by FastQC (version 0.11.5)