##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062668_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2082543
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206616622081754	32.0	32.0	32.0	32.0	32.0
2	31.34322844714371	32.0	32.0	32.0	32.0	32.0
3	31.47259240265387	32.0	32.0	32.0	32.0	32.0
4	31.594941857142924	32.0	32.0	32.0	32.0	32.0
5	31.514359607460687	32.0	32.0	32.0	32.0	32.0
6	35.119418422572785	36.0	36.0	36.0	36.0	36.0
7	35.13695803640069	36.0	36.0	36.0	36.0	36.0
8	35.07253631737736	36.0	36.0	36.0	36.0	36.0
9	35.21435811889599	36.0	36.0	36.0	36.0	36.0
10	35.08564144894007	36.0	36.0	36.0	36.0	36.0
11	35.22785988092443	36.0	36.0	36.0	36.0	36.0
12	35.131316376180465	36.0	36.0	36.0	36.0	36.0
13	35.17246750727356	36.0	36.0	36.0	36.0	36.0
14	35.131215537926465	36.0	36.0	36.0	36.0	36.0
15	35.09146941983911	36.0	36.0	36.0	36.0	36.0
16	35.10372991097903	36.0	36.0	36.0	36.0	36.0
17	35.079083601154935	36.0	36.0	36.0	36.0	36.0
18	35.085662096773035	36.0	36.0	36.0	36.0	36.0
19	35.071098171802454	36.0	36.0	36.0	36.0	36.0
20	35.056338332509824	36.0	36.0	36.0	36.0	36.0
21	35.04254269899829	36.0	36.0	36.0	36.0	36.0
22	35.021836283812625	36.0	36.0	36.0	36.0	36.0
23	34.96731592096778	36.0	36.0	36.0	36.0	36.0
24	34.946221518595294	36.0	36.0	36.0	32.0	36.0
25	34.90899203521848	36.0	36.0	36.0	32.0	36.0
26	34.85652349075145	36.0	36.0	36.0	32.0	36.0
27	34.82927411342767	36.0	36.0	36.0	32.0	36.0
28	34.80635357829346	36.0	36.0	36.0	32.0	36.0
29	34.771696910940136	36.0	36.0	36.0	32.0	36.0
30	34.75732842010945	36.0	36.0	36.0	32.0	36.0
31	34.746171867759756	36.0	36.0	36.0	32.0	36.0
32	34.70093006482939	36.0	36.0	36.0	32.0	36.0
33	34.67501127227625	36.0	36.0	36.0	32.0	36.0
34	34.652502253254795	36.0	36.0	36.0	32.0	36.0
35	34.60276354437819	36.0	36.0	36.0	32.0	36.0
36	34.566006080066536	36.0	36.0	36.0	32.0	36.0
37	34.532160920566824	36.0	36.0	36.0	32.0	36.0
38	34.481942509710485	36.0	36.0	36.0	32.0	36.0
39	34.41658731656441	36.0	36.0	36.0	32.0	36.0
40	34.374320242127055	36.0	36.0	36.0	32.0	36.0
41	34.354850296008294	36.0	36.0	36.0	32.0	36.0
42	34.25935887038107	36.0	36.0	36.0	32.0	36.0
43	34.281223004759084	36.0	36.0	36.0	32.0	36.0
44	34.26283298832245	36.0	36.0	36.0	32.0	36.0
45	34.14602147470664	36.0	36.0	36.0	32.0	36.0
46	34.19981580212269	36.0	36.0	36.0	32.0	36.0
47	34.133351868364784	36.0	36.0	36.0	32.0	36.0
48	34.11334171731388	36.0	36.0	36.0	32.0	36.0
49	34.126473258895494	36.0	36.0	36.0	32.0	36.0
50	33.5818621752348	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	13.0
21	50.0
22	192.0
23	589.0
24	1681.0
25	4006.0
26	8364.0
27	16059.0
28	25734.0
29	36742.0
30	51646.0
31	73097.0
32	108002.0
33	166586.0
34	366133.0
35	1223643.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56501067134375	17.818982889350362	11.64449153112171	25.97151490818418
2	15.63891830114792	20.585748139824677	37.224203047830336	26.55113051119707
3	18.682078004894034	24.57729422204466	28.576456781539434	28.164170991521875
4	12.086027763252225	15.904927499487403	36.42864448506798	35.5804002521924
5	13.898632585257541	37.19337367823858	33.84155813349352	15.066435603010358
6	34.197649784012924	35.420248044207725	16.974769175215553	13.407332996563797
7	29.8010653321444	30.776651430486666	21.10938405593546	18.31289918143347
8	28.16724552626284	33.1425569604085	19.75551045044448	18.934687062884176
9	27.14714654151199	14.405704948229161	18.590444471014525	39.85670403924433
10	15.291833109808536	27.3466142115673	32.43198339722157	24.929569281402593
11	37.11039820066141	21.349667209752692	21.841565816408114	19.698368773177794
12	23.811705208487894	24.18360629288327	29.416535456890923	22.58815304173791
13	29.70786197451865	19.743938060342572	25.40643818639039	25.141761778748382
14	23.357933065487725	19.66014627309016	25.71951695595241	31.262403705469705
15	25.246537526476043	27.142776883838653	23.16806903867051	24.442616551014794
16	25.667609264250483	26.023088118708714	24.412653184111925	23.896649432928875
17	23.719702306266903	26.1852936530002	26.029714632542998	24.06528940818989
18	24.8487066053378	24.822152531784457	27.176053507658665	23.153087355219075
19	24.882847557049242	26.35700679409741	25.634572731511422	23.125572917341923
20	24.735527669776808	24.46845035132528	26.74302523405279	24.052996744845125
21	25.87403957565342	25.042556144098825	24.733318831832044	24.35008544841571
22	24.587679582126277	25.34238188599227	25.539208554157106	24.53072997772435
23	23.506117280651587	24.961069231223558	25.795049610020058	25.737763878104797
24	23.75115423787168	26.21741783963164	25.580936384026643	24.450491538470036
25	24.717904984434895	24.957852010738794	25.302862894067495	25.021380110758816
26	23.794610723524077	25.52619561756948	26.10534332304303	24.573850335863412
27	25.11890510784171	25.63759787913143	25.307712733902733	23.935784279124128
28	24.151290033387067	24.816006200112074	26.135162635297327	24.897541131203532
29	23.793362249903126	25.758747838580042	26.38164013900313	24.0662497725137
30	24.609047688331046	25.21585388633032	26.405889338179335	23.769209087159304
31	24.12151873934896	25.479906057161845	25.45191143712279	24.94666376636641
32	23.915232482594597	25.593421120236172	25.61282047957713	24.8785259175921
33	23.628035531559252	25.14104150550553	25.971516554520125	25.259406408415096
34	23.945387922362226	25.664103934468578	26.46053406820411	23.92997407496508
35	25.167931706572205	25.20456960552555	25.905731598339145	23.721767089563098
36	24.141446299068015	25.903714833259144	25.67678074354287	24.27805812412997
37	25.155687061443626	25.544538576154252	25.575414289164737	23.72436007323738
38	24.0642330074337	25.50353101952757	25.66122284149715	24.77101313154158
39	24.4693626974329	25.35928429809132	25.62300034140952	24.54835266306626
40	24.81951152989398	25.45426432971612	25.6018723262857	24.124351814104198
41	23.549621784520173	25.762061095497184	26.314942836714533	24.373374283268102
42	25.249370601231284	25.778291252569574	25.730465109243845	23.241873036955297
43	24.406987034601446	24.633248869291055	25.630635237783807	25.329128858323696
44	24.08272002066704	25.26660914084367	26.19384089548211	24.45682994300718
45	24.409676054708115	25.520337395194243	25.665880608467628	24.404105941630018
46	23.805988747389605	25.37138560190229	26.257947630272472	24.564678020435633
47	24.35038525033349	24.85755389327082	26.544098510378184	24.247962346017513
48	24.556515759818645	26.551096423939384	24.9906484523969	23.901739363845067
49	23.571758182183995	25.733826384377178	26.381688157219322	24.312727276219505
50	23.507953260966648	26.732330008230328	25.001368519818566	24.758348210984458
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	32.0
2	44.0
3	164.0
4	284.0
5	339.0
6	394.0
7	356.5
8	319.0
9	459.0
10	599.0
11	872.0
12	1145.0
13	2005.5
14	2866.0
15	3955.5
16	5045.0
17	5874.0
18	6703.0
19	7482.0
20	8261.0
21	9650.0
22	11039.0
23	12237.5
24	13436.0
25	15022.0
26	16608.0
27	19851.0
28	23094.0
29	26480.5
30	29867.0
31	33323.5
32	36780.0
33	42767.0
34	48754.0
35	55498.5
36	62243.0
37	72595.0
38	82947.0
39	89636.0
40	96325.0
41	105769.5
42	115214.0
43	120388.5
44	125563.0
45	139195.0
46	152827.0
47	163185.5
48	173544.0
49	172263.5
50	170983.0
51	157864.0
52	144745.0
53	139833.0
54	134921.0
55	134449.5
56	133978.0
57	127574.0
58	121170.0
59	109993.5
60	98817.0
61	87138.0
62	75459.0
63	65013.5
64	54568.0
65	47049.5
66	39531.0
67	34201.5
68	28872.0
69	26609.5
70	24347.0
71	19024.5
72	13702.0
73	12393.5
74	11085.0
75	8084.0
76	5083.0
77	4645.0
78	4207.0
79	3610.0
80	3013.0
81	2377.0
82	1741.0
83	1440.5
84	1140.0
85	938.5
86	737.0
87	534.0
88	331.0
89	215.0
90	99.0
91	74.0
92	49.0
93	33.0
94	17.0
95	13.0
96	9.0
97	10.5
98	12.0
99	9.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015894029559053523
2	0.003745420862858534
3	3.361275133334582E-4
4	2.8810929714296414E-4
5	0.0
6	5.282003780954342E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.920728647619761E-4
47	4.801821619049403E-5
48	0.0
49	0.0
50	4.801821619049403E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2082543.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.607889219148305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.08663417448606	40.252359666645646
2	14.689524848219405	15.749488414905443
3	4.749966476430786	7.639070299895094
4	2.036273904379298	4.366413835432321
5	1.0445964688256686	2.7999305889759984
6	0.6158274263444781	1.9807925069752799
7	0.36368243311165166	1.3647373309639819
8	0.2494286248153332	1.0697073669747914
9	0.1901142560665834	0.9172461580378377
>10	0.8278507763214337	7.864356437342261
>50	0.06739131552630212	2.554839158233864
>100	0.06718436991609601	7.736440136267418
>500	0.008553655686614025	3.2235602880611993
>1k	0.002971269870086973	2.481057811288787
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3919	0.1881833892505461	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3677	0.17656298093244654	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2108	0.1012223997295614	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.801821619049403E-5
2	0.0	0.0	0.0	0.0	4.801821619049403E-5
3	0.0	0.0	0.0	0.0	4.801821619049403E-5
4	0.0	0.0	0.0	0.0	4.801821619049403E-5
5	0.0	0.0	0.0	0.0	1.920728647619761E-4
6	0.0	0.0	0.0	0.0	1.920728647619761E-4
7	0.0	0.0	0.0	0.0	1.920728647619761E-4
8	0.0	0.0	0.0	0.0	2.4009108095247013E-4
9	0.0	0.0	0.0	0.0	2.8810929714296414E-4
10	0.0	0.0	0.0	0.0	2.8810929714296414E-4
11	0.0	0.0	0.0	1.920728647619761E-4	2.8810929714296414E-4
12	0.0	0.0	0.0	1.920728647619761E-4	4.321639457144462E-4
13	0.0	0.0	0.0	1.920728647619761E-4	5.282003780954342E-4
14	0.0	0.0	0.0	2.8810929714296414E-4	5.282003780954342E-4
15	0.0	0.0	0.0	4.321639457144462E-4	5.762185942859283E-4
16	0.0	0.0	0.0	4.8018216190494025E-4	6.242368104764223E-4
17	0.0	0.0	0.0	6.242368104764223E-4	6.242368104764223E-4
18	0.0	0.0	0.0	9.603643238098805E-4	7.202732428574104E-4
19	0.0	0.0	0.0	0.0010083825400003744	7.202732428574104E-4
20	0.0	0.0	0.0	0.0015365829180958089	8.163096752383985E-4
21	0.0	0.0	0.0	0.0022568561609532193	9.603643238098805E-4
22	0.0	0.0	0.0	0.0031211840523821116	9.603643238098805E-4
23	0.0	0.0	0.0	0.005954258807621259	0.0010083825400003744
24	0.0	0.0	0.0	0.009603643238098805	0.0010564007561908685
25	0.0	0.0	0.0	0.01157239010190906	0.0010564007561908685
26	0.0	0.0	0.0	0.01502970166762463	0.0011044189723813625
27	0.0	0.0	0.0	0.018631067881911684	0.0011044189723813625
28	0.0	0.0	0.0	0.026313982472390727	0.0011044189723813625
29	0.0	0.0	0.0	0.03827051830382374	0.0011044189723813625
30	0.0	0.0	0.0	0.060166824886689015	0.0011044189723813625
31	0.0	0.0	0.0	0.10026203540575153	0.0011044189723813625
32	0.0	0.0	0.0	0.14453483073338702	0.0011044189723813625
33	0.0	0.0	0.0	0.1889996989257845	0.0011044189723813625
34	0.0	0.0	0.0	0.249118505596283	0.0011524371885718566
35	0.0	0.0	0.0	0.3093333486991625	0.0011524371885718566
36	0.0	0.0	0.0	0.39600622892300424	0.0011524371885718566
37	0.0	0.0	0.0	0.5207095363697172	0.0011524371885718566
38	0.0	0.0	0.0	0.6749920649897745	0.0012004554047623506
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	80	1.3087083E-7	24.754026	1
CGCAATA	555	0.0	24.180048	36
TATAACG	60	2.8724497E-4	22.001467	2
ATACGAA	620	0.0	21.290207	40
AATACGA	645	0.0	21.147171	39
CGGTCCA	670	0.0	21.01481	10
CAATACG	655	0.0	20.488438	38
CGAATGC	650	0.0	19.969122	43
CTAGCGG	650	0.0	19.969122	29
TACGAAT	685	0.0	19.9123	41
TCTAGCG	655	0.0	19.816687	28
GCAATAC	705	0.0	19.659468	37
TAGCGGC	675	0.0	19.229525	30
CGCCGGT	685	0.0	18.306469	7
GCGCAAT	755	0.0	17.774736	35
CCGGTCC	780	0.0	17.769135	9
TAGGACG	310	0.0	17.741838	4
TCGTATA	75	0.0012913425	17.599905	44
GGCGTTA	425	0.0	17.08226	42
CGGCGCA	995	0.0	17.025034	33
>>END_MODULE