FastQCFastQC Report
Thu 2 Feb 2017
SRR4062667_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062667_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909102
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13150.14464823529152945No Hit
GTACATGGAAGCAGTGGTATCAACG10080.11087864728050317No Hit
GCGCAAGACGGACCAGAGCGAAAGC9320.1025187492712589No Hit
TATCAACGCAGAGTACTTTTTTTTT9210.1013087640330788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTT551.1265049E-515.54658114
AATCGTA508.702207E-515.20110113
ATCGCCA851.708031E-814.53286616
TACGCAG400.005282045314.2478945
CGAACGA852.6870475E-713.41495216
GCAAGAC2050.012.971553
GTACGAA604.075243E-412.6717719
TGTACGA604.079032E-412.67037418
ACGAACG1051.987246E-812.66758315
AAGACGG1950.012.6647955
TATACAC1405.2750693E-1112.21046453
CGGTCCA1251.4060788E-912.16021210
AGACCGT550.003066358612.091126
ATATACT550.003072686712.0877914
CGTTATT951.0434997E-611.99492552
CATTCCG802.8627801E-511.8752069
TTAATCC802.8708562E-511.8719384
CGCAAGA1850.011.8058172
TCGCCAG1052.7045826E-711.765347517
GCGTTAT1052.729812E-711.756931
Produced by FastQC (version 0.11.5)