##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062667_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	909102
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90694773523763	32.0	32.0	32.0	32.0	32.0
2	30.397622049011	32.0	32.0	32.0	32.0	32.0
3	30.321508477596574	32.0	32.0	32.0	21.0	32.0
4	30.459670092024876	32.0	32.0	32.0	32.0	32.0
5	30.019174966065414	32.0	32.0	32.0	21.0	32.0
6	33.663927700082056	36.0	36.0	36.0	27.0	36.0
7	33.50354305677471	36.0	36.0	36.0	21.0	36.0
8	33.498625016774795	36.0	36.0	36.0	21.0	36.0
9	33.839639556397415	36.0	36.0	36.0	32.0	36.0
10	33.234155243306034	36.0	36.0	36.0	21.0	36.0
11	33.7782867049022	36.0	36.0	36.0	32.0	36.0
12	33.40405257055864	36.0	36.0	36.0	21.0	36.0
13	33.6327166808565	36.0	36.0	36.0	27.0	36.0
14	33.47287103097342	36.0	36.0	36.0	27.0	36.0
15	33.37443983183405	36.0	36.0	36.0	21.0	36.0
16	33.38107385089902	36.0	36.0	36.0	21.0	36.0
17	33.26071771924383	36.0	36.0	36.0	21.0	36.0
18	33.30846813668873	36.0	36.0	36.0	21.0	36.0
19	33.306055866118434	36.0	36.0	36.0	21.0	36.0
20	33.2861890084941	36.0	36.0	36.0	21.0	36.0
21	33.260360223605275	36.0	36.0	36.0	21.0	36.0
22	33.214225686446625	36.0	36.0	36.0	21.0	36.0
23	33.153350229127206	36.0	36.0	36.0	21.0	36.0
24	33.102232752760415	36.0	36.0	36.0	14.0	36.0
25	32.585910051897365	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	44.0
5	88.0
6	142.0
7	43.0
8	145.0
9	174.0
10	86.0
11	40.0
12	68.0
13	58.0
14	201.0
15	366.0
16	608.0
17	913.0
18	1308.0
19	1752.0
20	2380.0
21	3220.0
22	4407.0
23	5799.0
24	7943.0
25	10917.0
26	14910.0
27	18937.0
28	25674.0
29	34697.0
30	44878.0
31	61940.0
32	87841.0
33	119722.0
34	219187.0
35	240610.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.136711066520576	18.655639309492063	11.13307635102727	26.074573272960095
2	17.262678052649353	20.437237575502902	36.68635343114014	25.6137309407076
3	18.90680362880737	24.023853237248627	28.964126640845095	28.105216493098904
4	12.739266230058627	15.627568134287225	36.56077106461439	35.07239457103975
5	14.468595020406278	36.564373028282695	33.986546932236195	14.980485019074836
6	33.365979914649365	36.11489542456329	16.979780394884244	13.539344265903102
7	29.509580753956023	31.012517501607068	21.274579300992418	18.203322443444492
8	27.69430967345604	33.04368121312605	19.990840343443082	19.27116876997482
9	27.423810514749036	14.273162385257463	19.206389577958863	39.09663752203464
10	15.830799540558488	26.476913945018993	32.18535359486553	25.50693291955699
11	36.914493760730544	21.465047206122204	22.783052269781365	18.83740676336589
12	24.30406097520679	24.21925080679803	28.98877642057032	22.487911797424857
13	29.03547317760818	19.861895794099187	25.577691875640134	25.524939152652504
14	23.0939513717647	20.228504467555293	25.2070780437251	31.470466116954903
15	25.02937474465721	27.458531229730877	22.528639687785283	24.983454337826632
16	25.54521147730176	25.94032736915365	24.078943890900444	24.43551726264415
17	23.71174667646116	25.897666611967274	25.82619029001325	24.564396421558314
18	24.527757432912427	24.955421799538524	26.839325737509018	23.677495030040035
19	25.371224153084672	25.31703566967934	25.59128226252338	23.720457914712615
20	25.183256682701625	24.892843297915455	25.44261334602033	24.481286673362586
21	26.25477862743392	24.56349420507099	24.477916711180914	24.703810456314176
22	25.409185946343708	25.13488980786051	24.92746023807583	24.52846400771995
23	24.213041189079398	24.732393249194264	25.71679396207794	25.337771599648406
24	24.449427757321963	25.51240804521108	25.340806834089825	24.69735736337713
25	24.525743765061435	24.82642177745862	25.72768484538762	24.920149612092324
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	79.0
1	79.0
2	230.5
3	382.0
4	382.0
5	382.0
6	998.0
7	1614.0
8	1614.0
9	1614.0
10	1906.5
11	2199.0
12	2199.0
13	2199.0
14	2915.5
15	3632.0
16	3632.0
17	3632.0
18	5585.5
19	7539.0
20	7539.0
21	7539.0
22	12044.0
23	16549.0
24	16549.0
25	16549.0
26	24343.0
27	32137.0
28	32137.0
29	32137.0
30	39005.0
31	45873.0
32	45873.0
33	45873.0
34	55003.5
35	64134.0
36	64134.0
37	64134.0
38	70264.0
39	76394.0
40	76394.0
41	76394.0
42	84887.0
43	93380.0
44	93380.0
45	93380.0
46	104963.0
47	116546.0
48	116546.0
49	116546.0
50	115620.5
51	114695.0
52	114695.0
53	114695.0
54	105855.0
55	97015.0
56	97015.0
57	97015.0
58	88290.0
59	79565.0
60	79565.0
61	79565.0
62	71028.5
63	62492.0
64	62492.0
65	62492.0
66	52514.5
67	42537.0
68	42537.0
69	42537.0
70	32856.0
71	23175.0
72	23175.0
73	23175.0
74	18259.5
75	13344.0
76	13344.0
77	13344.0
78	10861.0
79	8378.0
80	8378.0
81	8378.0
82	6110.5
83	3843.0
84	3843.0
85	3843.0
86	2972.0
87	2101.0
88	2101.0
89	2101.0
90	1436.0
91	771.0
92	771.0
93	771.0
94	509.5
95	248.0
96	248.0
97	248.0
98	364.0
99	480.0
100	480.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009899879221473498
2	0.0034099583985075384
3	0.004729942294704004
4	0.013749832252046526
5	0.0344295799591245
6	0.04179949004622144
7	0.06753917602205253
8	0.08480896533062296
9	0.09602882844829293
10	0.1147286003110762
11	0.11692857347140365
12	0.13111840035551567
13	0.12011853455387844
14	0.12220850905618952
15	0.11098864593851955
16	0.11153863922860141
17	0.12198851174015676
18	0.12616846074477892
19	0.12759844329899175
20	0.1194585426057802
21	0.12682845269287715
22	0.14563822321367678
23	0.1363983359403015
24	0.1301284124333683
25	0.12693845135089352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	909102.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.101475059791156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.17373177249402	50.33893184101397
2	9.293666743165428	10.98538826470404
3	2.285943527965457	4.053079032184245
4	0.9552235348333715	2.2582047968192023
5	0.4945187084481632	1.4613392556974625
6	0.33064710229912014	1.1725038882074201
7	0.22161495816981608	0.9168439646205031
8	0.15340050097619287	0.7252956706083146
9	0.11687665933805454	0.6216824670245763
>10	0.7624677932673267	8.893577522281335
>50	0.1058636600229691	4.477619774659509
>100	0.10064976512612506	11.795940814722501
>500	0.005023186039173842	2.04416792695476
>1k	3.7208785475361793E-4	0.25542478050217343
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1315	0.14464823529152945	No Hit
GTACATGGAAGCAGTGGTATCAACG	1008	0.11087864728050317	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	932	0.1025187492712589	No Hit
TATCAACGCAGAGTACTTTTTTTTT	921	0.1013087640330788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.199973160327444E-4
2	0.0	0.0	0.0	0.0	2.199973160327444E-4
3	0.0	0.0	0.0	0.0	2.199973160327444E-4
4	0.0	0.0	0.0	0.0	2.199973160327444E-4
5	0.0	0.0	0.0	0.0	2.199973160327444E-4
6	0.0	0.0	0.0	0.0	2.199973160327444E-4
7	0.0	0.0	0.0	0.0	2.199973160327444E-4
8	0.0	0.0	0.0	0.0	2.199973160327444E-4
9	0.0	0.0	0.0	1.099986580163722E-4	2.199973160327444E-4
10	0.0	0.0	0.0	3.299959740491166E-4	2.199973160327444E-4
11	0.0	0.0	0.0	3.299959740491166E-4	2.199973160327444E-4
12	0.0	0.0	0.0	3.299959740491166E-4	2.199973160327444E-4
13	0.0	0.0	0.0	3.299959740491166E-4	3.299959740491166E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGTT	55	1.1265049E-5	15.546581	14
AATCGTA	50	8.702207E-5	15.201101	13
ATCGCCA	85	1.708031E-8	14.532866	16
TACGCAG	40	0.0052820453	14.247894	5
CGAACGA	85	2.6870475E-7	13.414952	16
GCAAGAC	205	0.0	12.97155	3
GTACGAA	60	4.075243E-4	12.67177	19
TGTACGA	60	4.079032E-4	12.670374	18
ACGAACG	105	1.987246E-8	12.667583	15
AAGACGG	195	0.0	12.664795	5
TATACAC	140	5.2750693E-11	12.2104645	3
CGGTCCA	125	1.4060788E-9	12.160212	10
AGACCGT	55	0.0030663586	12.09112	6
ATATACT	55	0.0030726867	12.087791	4
CGTTATT	95	1.0434997E-6	11.9949255	2
CATTCCG	80	2.8627801E-5	11.875206	9
TTAATCC	80	2.8708562E-5	11.871938	4
CGCAAGA	185	0.0	11.805817	2
TCGCCAG	105	2.7045826E-7	11.7653475	17
GCGTTAT	105	2.729812E-7	11.75693	1
>>END_MODULE