FastQCFastQC Report
Thu 2 Feb 2017
SRR4062667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909102
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG9740.10713869290794652No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC9640.1060387063277828No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9370.10306874256134076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC551.5932346E-423.9999123
ACTAGAC956.8627014E-720.8420283
GTACTAC1152.0177686E-719.1324671
CGCTTCG2550.018.9803232
ATCGGGG708.121414E-418.8570724
ACGAATT708.121414E-418.8570721
TAAACGC2700.018.74067128
TAACGGC1801.4551915E-1118.33326336
GTAAACG2650.018.26408227
TACTAGA851.429777E-418.1185762
GTCCTAA2950.017.9002081
GAGCGAA3600.017.72215516
AGCGAAA3600.017.72215517
GAGCGTT1002.481995E-517.5999367
ATCCCGT750.001290568817.59993420
GCGAAAG3650.017.47938718
TTAACGG1903.274181E-1117.36835735
CGTAAAC1903.274181E-1117.36835732
TATACAG1903.274181E-1117.3683575
AATCCCG902.2146749E-417.11104619
Produced by FastQC (version 0.11.5)