##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062667_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 909102 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.955172246898588 32.0 32.0 32.0 32.0 32.0 2 30.741319455902637 32.0 32.0 32.0 32.0 32.0 3 31.10958506306223 32.0 32.0 32.0 32.0 32.0 4 31.386278987396352 32.0 32.0 32.0 32.0 32.0 5 31.188694997921026 32.0 32.0 32.0 32.0 32.0 6 34.79678737919397 36.0 36.0 36.0 32.0 36.0 7 34.81316507938603 36.0 36.0 36.0 32.0 36.0 8 34.71106102505549 36.0 36.0 36.0 32.0 36.0 9 34.96455843238712 36.0 36.0 36.0 32.0 36.0 10 34.69294314609362 36.0 36.0 36.0 32.0 36.0 11 34.9582159097659 36.0 36.0 36.0 36.0 36.0 12 34.80421228861008 36.0 36.0 36.0 32.0 36.0 13 34.90589724805358 36.0 36.0 36.0 32.0 36.0 14 34.85077912049473 36.0 36.0 36.0 32.0 36.0 15 34.759492334193524 36.0 36.0 36.0 32.0 36.0 16 34.80103882732631 36.0 36.0 36.0 32.0 36.0 17 34.76060772058581 36.0 36.0 36.0 32.0 36.0 18 34.78277245017611 36.0 36.0 36.0 32.0 36.0 19 34.7746380494158 36.0 36.0 36.0 32.0 36.0 20 34.772421576456765 36.0 36.0 36.0 32.0 36.0 21 34.778107407089635 36.0 36.0 36.0 32.0 36.0 22 34.745266207752266 36.0 36.0 36.0 32.0 36.0 23 34.6971142952056 36.0 36.0 36.0 32.0 36.0 24 34.665696478502966 36.0 36.0 36.0 32.0 36.0 25 34.6456481230929 36.0 36.0 36.0 32.0 36.0 26 34.57984582588092 36.0 36.0 36.0 32.0 36.0 27 34.56488710837728 36.0 36.0 36.0 32.0 36.0 28 34.547960514881716 36.0 36.0 36.0 32.0 36.0 29 34.51449672313998 36.0 36.0 36.0 32.0 36.0 30 34.50318006120325 36.0 36.0 36.0 32.0 36.0 31 34.51002087774529 36.0 36.0 36.0 32.0 36.0 32 34.46033118395955 36.0 36.0 36.0 32.0 36.0 33 34.44077342256424 36.0 36.0 36.0 32.0 36.0 34 34.419198285780915 36.0 36.0 36.0 32.0 36.0 35 34.400746010898665 36.0 36.0 36.0 32.0 36.0 36 34.363860160906036 36.0 36.0 36.0 32.0 36.0 37 34.36059760070927 36.0 36.0 36.0 32.0 36.0 38 34.314716060464065 36.0 36.0 36.0 32.0 36.0 39 34.302576608565374 36.0 36.0 36.0 32.0 36.0 40 34.30447408541616 36.0 36.0 36.0 32.0 36.0 41 34.259311936394376 36.0 36.0 36.0 32.0 36.0 42 34.22725612747524 36.0 36.0 36.0 32.0 36.0 43 34.23499563305327 36.0 36.0 36.0 32.0 36.0 44 34.175921953752166 36.0 36.0 36.0 32.0 36.0 45 34.135977041080096 36.0 36.0 36.0 32.0 36.0 46 34.140254888890354 36.0 36.0 36.0 32.0 36.0 47 34.12596826318719 36.0 36.0 36.0 32.0 36.0 48 34.0753886802581 36.0 36.0 36.0 32.0 36.0 49 34.06512800543833 36.0 36.0 36.0 32.0 36.0 50 33.40775512538747 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 4.0 21 20.0 22 71.0 23 229.0 24 567.0 25 1357.0 26 2928.0 27 5630.0 28 10142.0 29 16451.0 30 25557.0 31 39926.0 32 62967.0 33 109430.0 34 221607.0 35 412215.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.76683854033164 18.639363433043528 11.702187346328552 25.891610680296278 2 16.629541016967796 20.701262272089764 36.86912520969117 25.80007150125127 3 18.65143400223738 24.008386325361705 29.60524651330603 27.734933159094883 4 12.955120448795512 16.253987460125398 37.04410955890441 33.74678253217468 5 14.91944798273461 36.1206993274682 33.72833851427013 15.231514175527058 6 33.645730552998074 36.434865729695005 16.69048145471505 13.228922262591864 7 29.648048293810813 30.882123238096494 21.067712973901717 18.40211549419097 8 27.6169230735385 33.227514624321586 19.726609335366106 19.428952966773807 9 27.202338131474797 14.18597693108144 18.97366852124404 39.638016416199726 10 15.693728536511856 26.55081608004382 32.07021874333133 25.68523664011299 11 37.12553706844777 21.24327083209585 22.704493005185338 18.92669909427105 12 24.194754823991147 23.99851721808994 28.95241678051528 22.854311177403634 13 29.171534107283893 19.674249974150314 25.456219434122907 25.697996484442893 14 23.018869169796126 20.237113107220093 25.0147948195032 31.729222903480576 15 24.997415031536615 27.4563250328346 22.329837575981575 25.21642235964721 16 25.611977533874086 25.991362905372554 23.879058675484156 24.5176008852692 17 23.839459158598263 25.984762985891575 25.570508039801915 24.605269815708247 18 24.73814819459203 24.960455482443113 26.454127259647432 23.847269063317427 19 25.367780513077744 25.46578931737033 25.377790390957227 23.7886397785947 20 25.260201825537727 24.869596590921592 25.185512736744613 24.684688846796067 21 26.35688844596096 24.669949026621875 24.244804213388598 24.72835831402857 22 25.34292081636604 25.2908914511243 24.797657468578883 24.56853026393078 23 24.28869367793713 24.790177559833772 25.51880867053422 25.40232009169488 24 24.482291316045945 25.609227567423677 25.209052449560115 24.699428666970263 25 24.615059696271704 24.91656601789458 25.494388968454583 24.973985317379128 26 24.22863441066019 25.50065889196152 25.91018389575647 24.36052280162182 27 25.060774258554048 25.33533090896291 24.959025499888902 24.644869332594144 28 24.092566070693938 25.127543443969984 25.87751429432561 24.902376191010468 29 24.41453214270786 25.22159229657398 25.469749269058916 24.89412629165924 30 24.457871613966308 25.281981559824967 25.920633768268026 24.339513057940692 31 24.70228863207869 25.133813367476915 24.980365239544078 25.183532760900317 32 24.315423351835108 25.324001047187224 25.17616285081322 25.184412750164448 33 24.20344471797444 25.047024426302002 25.660926936691375 25.088603919032188 34 24.603839833154034 25.088383921716158 26.057142102866344 24.250634142263465 35 25.44422958039912 25.02480469738269 25.456549430096953 24.074416292121235 36 24.23083438382052 25.237652100644368 25.506158824862336 25.025354690672774 37 25.240732063068833 25.152403140681685 24.88928635070652 24.717578445542966 38 24.281543765166063 25.19376263609584 25.581177909629503 24.94351568910859 39 24.67489896623261 25.02733466651707 24.907876123911286 25.389890243339032 40 25.0960838277773 25.00258496846339 25.743865924835717 24.1574652789236 41 24.089706105585513 25.158783062846634 26.158010872267358 24.593499959300498 42 25.136893329901376 25.639037203746113 25.48096913217659 23.743100334175924 43 24.14822539165022 24.675118963548645 25.87146436813471 25.305191276666424 44 24.080576216970154 25.07705405994047 25.71801624020187 25.124353482887507 45 24.19288484680487 25.41408994810263 25.775765535660465 24.61725966943203 46 24.310363413566353 24.796117487366654 25.64453713664693 25.248981962420057 47 24.515070916134825 24.6916187622511 25.929763656883388 24.86354666473069 48 24.756627969138776 25.508248799364647 25.246341994627663 24.48878123686891 49 23.882028639250603 25.23930208051462 25.784015435011693 25.094653845223085 50 23.658977385351022 25.62388557487012 25.534566566311113 25.18257047346775 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6.0 1 9.0 2 12.0 3 32.5 4 53.0 5 60.0 6 67.0 7 85.5 8 104.0 9 144.0 10 184.0 11 248.0 12 312.0 13 480.0 14 648.0 15 905.5 16 1163.0 17 1610.0 18 2057.0 19 2406.0 20 2755.0 21 3492.5 22 4230.0 23 4601.0 24 4972.0 25 6593.5 26 8215.0 27 10477.0 28 12739.0 29 14430.0 30 16121.0 31 16865.0 32 17609.0 33 20374.0 34 23139.0 35 26776.0 36 30413.0 37 35520.5 38 40628.0 39 42512.5 40 44397.0 41 47116.5 42 49836.0 43 49759.5 44 49683.0 45 53577.0 46 57471.0 47 60370.5 48 63270.0 49 63897.0 50 64524.0 51 62275.5 52 60027.0 53 58323.0 54 56619.0 55 57261.5 56 57904.0 57 57098.5 58 56293.0 59 51151.5 60 46010.0 61 40638.5 62 35267.0 63 31513.5 64 27760.0 65 24011.5 66 20263.0 67 17968.5 68 15674.0 69 14537.5 70 13401.0 71 10700.5 72 8000.0 73 7214.5 74 6429.0 75 4966.0 76 3503.0 77 3058.5 78 2614.0 79 2268.5 80 1923.0 81 1528.0 82 1133.0 83 964.5 84 796.0 85 635.5 86 475.0 87 364.5 88 254.0 89 173.0 90 92.0 91 60.5 92 29.0 93 22.0 94 15.0 95 9.5 96 4.0 97 5.5 98 7.0 99 4.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011109864459653592 2 0.0029699637664420495 3 3.2999597404911657E-4 4 2.199973160327444E-4 5 0.0 6 3.2999597404911657E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 1.099986580163722E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 909102.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.02547431761876 #Duplication Level Percentage of deduplicated Percentage of total 1 85.5318452946259 53.051532736603605 2 9.26346521624262 11.491416477244226 3 2.1709144422059405 4.039559939423766 4 0.8973381662209645 2.2263130153263018 5 0.48292852082692944 1.4976935282898163 6 0.3118084852108118 1.1604041514875287 7 0.2198495039358077 0.9545388832078167 8 0.15211500778747508 0.7548004407077131 9 0.10884511519848798 0.6076052907607864 >10 0.6808939846193869 8.22074475025942 >50 0.09017887489520753 3.9948032057685974 >100 0.08502476084324663 10.04315269114264 >500 0.004792627387175193 1.9574348897777891 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG 974 0.10713869290794652 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC 964 0.1060387063277828 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 937 0.10306874256134076 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.099986580163722E-4 7 0.0 0.0 0.0 0.0 1.099986580163722E-4 8 0.0 0.0 0.0 0.0 1.099986580163722E-4 9 0.0 0.0 0.0 0.0 1.099986580163722E-4 10 0.0 0.0 0.0 0.0 1.099986580163722E-4 11 0.0 0.0 0.0 0.0 1.099986580163722E-4 12 0.0 0.0 0.0 1.099986580163722E-4 3.299959740491166E-4 13 0.0 0.0 0.0 1.099986580163722E-4 3.299959740491166E-4 14 0.0 0.0 0.0 1.099986580163722E-4 3.299959740491166E-4 15 0.0 0.0 0.0 1.099986580163722E-4 4.399946320654888E-4 16 0.0 0.0 0.0 1.099986580163722E-4 4.399946320654888E-4 17 0.0 0.0 0.0 1.099986580163722E-4 4.399946320654888E-4 18 0.0 0.0 0.0 1.099986580163722E-4 4.399946320654888E-4 19 0.0 0.0 0.0 1.099986580163722E-4 5.49993290081861E-4 20 0.0 0.0 0.0 1.099986580163722E-4 7.699906061146054E-4 21 0.0 0.0 0.0 2.199973160327444E-4 7.699906061146054E-4 22 0.0 0.0 0.0 2.199973160327444E-4 7.699906061146054E-4 23 0.0 0.0 0.0 5.49993290081861E-4 7.699906061146054E-4 24 0.0 0.0 0.0 0.001099986580163722 7.699906061146054E-4 25 0.0 0.0 0.0 0.0012099852381800941 7.699906061146054E-4 26 0.0 0.0 0.0 0.0017599785282619552 9.899879221473497E-4 27 0.0 0.0 0.0 0.003409958398507538 9.899879221473497E-4 28 0.0 0.0 0.0 0.007149912771064193 9.899879221473497E-4 29 0.0 0.0 0.0 0.018149778572701412 9.899879221473497E-4 30 0.0 0.0 0.0 0.036629553119451944 9.899879221473497E-4 31 0.0 0.0 0.0 0.08106901095806632 9.899879221473497E-4 32 0.0 0.0 0.0 0.1380483158105471 9.899879221473497E-4 33 0.0 0.0 0.0 0.19293764616071685 9.899879221473497E-4 34 0.0 0.0 0.0 0.26421677655532605 9.899879221473497E-4 35 0.0 0.0 0.0 0.33219594720944406 9.899879221473497E-4 36 0.0 0.0 0.0 0.436804670983014 9.899879221473497E-4 37 0.0 0.0 0.0 0.5888228163616404 9.899879221473497E-4 38 0.0 0.0 0.0 0.7956202934324201 9.899879221473497E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACAC 55 1.5932346E-4 23.999912 3 ACTAGAC 95 6.8627014E-7 20.842028 3 GTACTAC 115 2.0177686E-7 19.132467 1 CGCTTCG 255 0.0 18.98032 32 ATCGGGG 70 8.121414E-4 18.85707 24 ACGAATT 70 8.121414E-4 18.85707 21 TAAACGC 270 0.0 18.740671 28 TAACGGC 180 1.4551915E-11 18.333263 36 GTAAACG 265 0.0 18.264082 27 TACTAGA 85 1.429777E-4 18.118576 2 GTCCTAA 295 0.0 17.900208 1 GAGCGAA 360 0.0 17.722155 16 AGCGAAA 360 0.0 17.722155 17 GAGCGTT 100 2.481995E-5 17.599936 7 ATCCCGT 75 0.0012905688 17.599934 20 GCGAAAG 365 0.0 17.479387 18 TTAACGG 190 3.274181E-11 17.368357 35 CGTAAAC 190 3.274181E-11 17.368357 32 TATACAG 190 3.274181E-11 17.368357 5 AATCCCG 90 2.2146749E-4 17.111046 19 >>END_MODULE