FastQCFastQC Report
Thu 2 Feb 2017
SRR4062666_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062666_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766441
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG34250.44687066584381574No Hit
GTATCAACGCAGAGTACTTTTTTTT29940.3906367222004042No Hit
TCCATGTACTCTGCGTTGATACCAC28560.3726314223795439No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25210.32892290469846996No Hit
TATCAACGCAGAGTACTTTTTTTTT19610.2558579199181672No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17590.22950233612241516No Hit
GAGTACATGGAAGCAGTGGTATCAA14050.18331482788629522No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12880.16804946499469625No Hit
CATGTACTCTGCGTTGATACCACTG11870.15487167309682023No Hit
GGTATCAACGCAGAGTACTTTTTTT10330.134778802282237No Hit
GTACATGGGGTGGTATCAACGCAAA10310.13451785590802162No Hit
GTCCTAAAGTGTGTATTTCTCATTT8630.1125983604739308No Hit
GCTTCCATGTACTCTGCGTTGATAC8510.11103268222863862No Hit
CATGGAAGCAGTGGTATCAACGCAG7820.10203003231820844No Hit
GCGTTGATACCACTGCTTCCATGTA7690.10033388088580857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGGG401.2814591E-519.00405118
GCGTGAG250.00602874319.0003269
AACCGCG504.5554552E-617.1002947
TTGCGCG402.7543365E-416.62854618
TGCGCGC456.745868E-414.78092919
CTATACC400.005279836714.2483844
CGTTATT602.5755677E-514.2437322
GACTAAA400.00529358114.2428021
TTTGCGC551.9545684E-413.82112917
GCGTTAT551.9665189E-413.8112021
TTAGACA500.001499616513.2984924
GGTCGGA751.476002E-512.6668857
GACAGTC604.0872174E-412.6668847
CGCGGTC604.0872174E-412.66688410
TAAACCA604.0917253E-412.6652314
TAGAACC853.9409315E-612.2927234
TGCACCG701.0893311E-412.2144975
TAGGGTC701.09066066E-412.2129014
GTTCTAA701.0946581E-412.2081161
ATTTGCG550.003059609612.09427716
Produced by FastQC (version 0.11.5)