##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062666_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	766441
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.998078129953903	32.0	32.0	32.0	32.0	32.0
2	30.647029582185713	32.0	32.0	32.0	32.0	32.0
3	30.6564445273674	32.0	32.0	32.0	32.0	32.0
4	30.701868767458944	32.0	32.0	32.0	32.0	32.0
5	30.513122340793355	32.0	32.0	32.0	32.0	32.0
6	34.12506768296581	36.0	36.0	36.0	32.0	36.0
7	33.95639064194113	36.0	36.0	36.0	32.0	36.0
8	33.944475830494454	36.0	36.0	36.0	32.0	36.0
9	34.10533752761139	36.0	36.0	36.0	32.0	36.0
10	33.771687579344004	36.0	36.0	36.0	32.0	36.0
11	34.159820521083816	36.0	36.0	36.0	32.0	36.0
12	33.91517155267007	36.0	36.0	36.0	32.0	36.0
13	34.04065414037088	36.0	36.0	36.0	32.0	36.0
14	33.93729067208044	36.0	36.0	36.0	32.0	36.0
15	33.88598600544595	36.0	36.0	36.0	32.0	36.0
16	33.885024418056965	36.0	36.0	36.0	32.0	36.0
17	33.80623687929012	36.0	36.0	36.0	32.0	36.0
18	33.83764047069507	36.0	36.0	36.0	32.0	36.0
19	33.812734444008086	36.0	36.0	36.0	32.0	36.0
20	33.7773357636139	36.0	36.0	36.0	32.0	36.0
21	33.73111302761726	36.0	36.0	36.0	32.0	36.0
22	33.681174415251796	36.0	36.0	36.0	27.0	36.0
23	33.66150036336783	36.0	36.0	36.0	27.0	36.0
24	33.64083341052997	36.0	36.0	36.0	27.0	36.0
25	33.15127191786452	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	32.0
5	65.0
6	109.0
7	47.0
8	127.0
9	125.0
10	105.0
11	32.0
12	62.0
13	45.0
14	221.0
15	239.0
16	395.0
17	528.0
18	716.0
19	1015.0
20	1541.0
21	2133.0
22	3153.0
23	4283.0
24	5915.0
25	7992.0
26	10412.0
27	13514.0
28	17695.0
29	23118.0
30	29679.0
31	40373.0
32	57456.0
33	82029.0
34	173705.0
35	289572.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.98133548371944	17.443832906648712	11.042128255916824	25.532703353715025
2	15.884029984146764	20.621603460311455	38.26253743125371	25.231829124288076
3	19.583328440371762	25.142124223813195	28.040845138418618	27.23370219739642
4	12.337554724761365	15.667038566684283	36.19826838132148	35.79713832723287
5	13.73150418892516	37.66286820319322	33.967817256100666	14.637810351780962
6	33.05112290251209	35.541545108635354	17.748973691252388	13.658358297600168
7	29.174968078189504	31.081268098817915	21.278856925009205	18.464906897983376
8	27.693221810868867	33.61083361083361	20.187133128309597	18.50881144998792
9	27.639884182440085	14.447417809104765	18.902240732832084	39.01045727562307
10	15.480649025429875	27.557715896577083	32.81194277854051	24.14969229945253
11	36.50400715867303	21.507501583921727	22.6744436679055	19.31404758949974
12	24.24020715803116	24.16795792037167	29.995845342210544	21.595989579386625
13	30.061267145656434	20.409144350097975	25.407576747224038	24.122011757021554
14	22.9478128641525	20.389404762526848	26.117474617100996	30.545307756219657
15	24.485535964804573	28.259143051959757	23.73601339683419	23.51930758640148
16	24.362949997387535	26.32190814567114	25.754872250378806	23.560269606562517
17	22.74842387711859	26.44437651374421	26.882398894297214	23.924800714839982
18	23.52287187205234	25.292838871339026	28.647388815292686	22.53690044131595
19	24.37122673986405	26.191928865105258	26.325711799680956	23.111132595349737
20	24.591050531810666	24.97759628372641	27.34938562870184	23.08196755576109
21	25.260693597819866	25.156050364618306	25.220325608981813	24.36293042858001
22	24.384905571620656	26.05891369142899	25.944055121659922	23.612125615290434
23	23.25526702596766	25.902596766291037	26.354270782296258	24.487865425445044
24	23.538726252954294	26.321675544250084	26.698858521761508	23.440739681034113
25	24.006736074185657	25.806114463615927	26.34372007404978	23.84342938814864
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	280.0
1	280.0
2	536.5
3	793.0
4	793.0
5	793.0
6	2236.0
7	3679.0
8	3679.0
9	3679.0
10	3599.0
11	3519.0
12	3519.0
13	3519.0
14	3356.5
15	3194.0
16	3194.0
17	3194.0
18	5072.0
19	6950.0
20	6950.0
21	6950.0
22	10883.5
23	14817.0
24	14817.0
25	14817.0
26	21412.0
27	28007.0
28	28007.0
29	28007.0
30	35328.0
31	42649.0
32	42649.0
33	42649.0
34	48336.5
35	54024.0
36	54024.0
37	54024.0
38	59246.0
39	64468.0
40	64468.0
41	64468.0
42	72769.0
43	81070.0
44	81070.0
45	81070.0
46	94516.5
47	107963.0
48	107963.0
49	107963.0
50	102924.0
51	97885.0
52	97885.0
53	97885.0
54	88244.0
55	78603.0
56	78603.0
57	78603.0
58	71054.0
59	63505.0
60	63505.0
61	63505.0
62	56129.0
63	48753.0
64	48753.0
65	48753.0
66	40239.5
67	31726.0
68	31726.0
69	31726.0
70	24081.0
71	16436.0
72	16436.0
73	16436.0
74	12761.0
75	9086.0
76	9086.0
77	9086.0
78	7135.0
79	5184.0
80	5184.0
81	5184.0
82	3650.5
83	2117.0
84	2117.0
85	2117.0
86	1519.0
87	921.0
88	921.0
89	921.0
90	644.5
91	368.0
92	368.0
93	368.0
94	235.5
95	103.0
96	103.0
97	103.0
98	222.0
99	341.0
100	341.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00874170353621479
2	0.004697034735876604
3	0.004436088361661237
4	0.013830157833414446
5	0.03314018952535159
6	0.04514372273925847
7	0.0661499058635955
8	0.088199874484794
9	0.0983767830791933
10	0.12055722488749948
11	0.1221229031327917
12	0.13503974865645235
13	0.12277526906833011
14	0.12590662555891452
15	0.11572971696451521
16	0.1149468778418691
17	0.12460189368783767
18	0.13086460666900648
19	0.13334359722405245
20	0.12303621544254549
21	0.1282551429268528
22	0.15030511154805132
23	0.13908441745679054
24	0.13438738272091394
25	0.1317779189787603
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	766441.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.19957775955117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.0319324975562	47.37856611136229
2	12.800779491430653	15.156014817716338
3	3.570074133138169	6.340406437562258
4	1.289202392437443	3.0528094911559926
5	0.6222444616960342	1.8418304697812222
6	0.35532499823470026	1.2621053917744514
7	0.21951757333615088	0.9096743356611009
8	0.1537561908151922	0.7281841259341086
9	0.12089220782514033	0.6441090891901393
>10	0.6749623021072662	7.7502447304903495
>50	0.08841273920278128	3.681892383935017
>100	0.06802617249862282	7.4113450083038215
>500	0.0024374198608711245	1.0277684230034314
>1k	0.0024374198608711245	2.8150491841293728
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3425	0.44687066584381574	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2994	0.3906367222004042	No Hit
TCCATGTACTCTGCGTTGATACCAC	2856	0.3726314223795439	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2521	0.32892290469846996	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1961	0.2558579199181672	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1759	0.22950233612241516	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1405	0.18331482788629522	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1288	0.16804946499469625	No Hit
CATGTACTCTGCGTTGATACCACTG	1187	0.15487167309682023	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1033	0.134778802282237	No Hit
GTACATGGGGTGGTATCAACGCAAA	1031	0.13451785590802162	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	863	0.1125983604739308	No Hit
GCTTCCATGTACTCTGCGTTGATAC	851	0.11103268222863862	No Hit
CATGGAAGCAGTGGTATCAACGCAG	782	0.10203003231820844	No Hit
GCGTTGATACCACTGCTTCCATGTA	769	0.10033388088580857	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3047318710768344E-4
2	0.0	0.0	0.0	0.0	1.3047318710768344E-4
3	0.0	0.0	0.0	0.0	1.3047318710768344E-4
4	0.0	0.0	0.0	0.0	1.3047318710768344E-4
5	0.0	0.0	0.0	0.0	1.3047318710768344E-4
6	0.0	0.0	0.0	0.0	1.3047318710768344E-4
7	0.0	0.0	0.0	0.0	1.3047318710768344E-4
8	0.0	0.0	0.0	0.0	1.3047318710768344E-4
9	0.0	0.0	0.0	0.0	1.3047318710768344E-4
10	0.0	0.0	0.0	0.0	1.3047318710768344E-4
11	0.0	0.0	0.0	0.0	1.3047318710768344E-4
12	0.0	0.0	0.0	0.0	1.3047318710768344E-4
13	0.0	0.0	0.0	1.3047318710768344E-4	1.3047318710768344E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGGG	40	1.2814591E-5	19.004051	18
GCGTGAG	25	0.006028743	19.000326	9
AACCGCG	50	4.5554552E-6	17.100294	7
TTGCGCG	40	2.7543365E-4	16.628546	18
TGCGCGC	45	6.745868E-4	14.780929	19
CTATACC	40	0.0052798367	14.248384	4
CGTTATT	60	2.5755677E-5	14.243732	2
GACTAAA	40	0.005293581	14.242802	1
TTTGCGC	55	1.9545684E-4	13.821129	17
GCGTTAT	55	1.9665189E-4	13.811202	1
TTAGACA	50	0.0014996165	13.298492	4
GGTCGGA	75	1.476002E-5	12.666885	7
GACAGTC	60	4.0872174E-4	12.666884	7
CGCGGTC	60	4.0872174E-4	12.666884	10
TAAACCA	60	4.0917253E-4	12.665231	4
TAGAACC	85	3.9409315E-6	12.292723	4
TGCACCG	70	1.0893311E-4	12.214497	5
TAGGGTC	70	1.09066066E-4	12.212901	4
GTTCTAA	70	1.0946581E-4	12.208116	1
ATTTGCG	55	0.0030596096	12.094277	16
>>END_MODULE