FastQCFastQC Report
Thu 2 Feb 2017
SRR4062666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766441
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA13080.17065892873684993No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC12660.16517905487832724No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8750.11416403871922301No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7910.1032042910021776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTT250.002352291535.19989424
TCGCGTT300.00574380229.33324623
ATACCGT1053.0649971E-923.047556
TGTAGGA6150.022.1787972
GGACCGT500.002579628721.9999356
TAGGACC4600.021.9999354
GTCCTAA2250.021.5138551
TACCGTC1057.7370714E-820.9523187
TTAGGAC3700.020.810753
TCCTACA5950.020.7058222
TGGAACG654.929996E-420.3076325
CCGCCGT654.929996E-420.30763224
TGGTATA1750.020.11422744
TACGACG1352.593879E-919.5554985
GATATAC2750.019.2024481
CTGTAGG6000.019.06911
GTCCTAC6300.018.8595471
GACGGTA1404.0745363E-918.8570868
GCGTATG708.1197824E-418.8570868
ACGACGG1404.0745363E-918.8570866
Produced by FastQC (version 0.11.5)