##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062666_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	766441
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.166372884540362	32.0	32.0	32.0	32.0	32.0
2	31.371620255179458	32.0	32.0	32.0	32.0	32.0
3	31.47898403138663	32.0	32.0	32.0	32.0	32.0
4	31.591517155267006	32.0	32.0	32.0	32.0	32.0
5	31.513277603886014	32.0	32.0	32.0	32.0	32.0
6	35.09423425938852	36.0	36.0	36.0	36.0	36.0
7	35.11653212706523	36.0	36.0	36.0	36.0	36.0
8	35.05902867931126	36.0	36.0	36.0	36.0	36.0
9	35.186726701729164	36.0	36.0	36.0	36.0	36.0
10	35.05476481555658	36.0	36.0	36.0	36.0	36.0
11	35.19739027531147	36.0	36.0	36.0	36.0	36.0
12	35.1131907087434	36.0	36.0	36.0	36.0	36.0
13	35.1381998092482	36.0	36.0	36.0	36.0	36.0
14	35.09762134332584	36.0	36.0	36.0	36.0	36.0
15	35.06478907052206	36.0	36.0	36.0	36.0	36.0
16	35.078629666210446	36.0	36.0	36.0	36.0	36.0
17	35.0605108547168	36.0	36.0	36.0	36.0	36.0
18	35.066585686308535	36.0	36.0	36.0	36.0	36.0
19	35.034030016661426	36.0	36.0	36.0	36.0	36.0
20	35.0145751597318	36.0	36.0	36.0	36.0	36.0
21	34.99665988641004	36.0	36.0	36.0	36.0	36.0
22	34.98041988881075	36.0	36.0	36.0	36.0	36.0
23	34.947176625467584	36.0	36.0	36.0	32.0	36.0
24	34.92652271994844	36.0	36.0	36.0	32.0	36.0
25	34.89904245727982	36.0	36.0	36.0	32.0	36.0
26	34.86100691377418	36.0	36.0	36.0	32.0	36.0
27	34.82372028636255	36.0	36.0	36.0	32.0	36.0
28	34.80992274682591	36.0	36.0	36.0	32.0	36.0
29	34.76963262664706	36.0	36.0	36.0	32.0	36.0
30	34.75232405364535	36.0	36.0	36.0	32.0	36.0
31	34.73230946674304	36.0	36.0	36.0	32.0	36.0
32	34.6816115004286	36.0	36.0	36.0	32.0	36.0
33	34.64460930456487	36.0	36.0	36.0	32.0	36.0
34	34.614532625472805	36.0	36.0	36.0	32.0	36.0
35	34.55834826164049	36.0	36.0	36.0	32.0	36.0
36	34.52309962541148	36.0	36.0	36.0	32.0	36.0
37	34.47560999476803	36.0	36.0	36.0	32.0	36.0
38	34.43610401844369	36.0	36.0	36.0	32.0	36.0
39	34.37242005581643	36.0	36.0	36.0	32.0	36.0
40	34.34085859185508	36.0	36.0	36.0	32.0	36.0
41	34.30835902567843	36.0	36.0	36.0	32.0	36.0
42	34.23315297589769	36.0	36.0	36.0	32.0	36.0
43	34.22314568244653	36.0	36.0	36.0	32.0	36.0
44	34.182449790655774	36.0	36.0	36.0	32.0	36.0
45	34.08731396154433	36.0	36.0	36.0	32.0	36.0
46	34.091841381136966	36.0	36.0	36.0	32.0	36.0
47	34.00803192939835	36.0	36.0	36.0	32.0	36.0
48	33.97332084270022	36.0	36.0	36.0	32.0	36.0
49	33.93776820394525	36.0	36.0	36.0	32.0	36.0
50	33.41286413435607	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	12.0
21	33.0
22	76.0
23	215.0
24	642.0
25	1477.0
26	3132.0
27	6027.0
28	10109.0
29	14643.0
30	20579.0
31	28152.0
32	40664.0
33	63084.0
34	132814.0
35	444778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.3104840972292	18.178864249986624	11.632844542634666	25.877807110149508
2	15.280813257846109	21.109761364511176	37.85482496715157	25.75460041049114
3	18.875319659725484	25.295652627733418	28.63485726214707	27.19417045039403
4	12.169641459214029	16.123636553415793	36.40219717133761	35.30452481603257
5	13.458831142905977	37.69787889739719	33.95903924763942	14.884250712057417
6	33.23317276328356	35.757453981806705	17.395973049282652	13.613400205627086
7	29.099173974252423	30.932322253115373	21.40399587182836	18.564507900803846
8	27.615824310025168	33.825565177228256	20.058295419999713	18.500315092746867
9	27.588424940732555	14.070489444066798	18.793357870990725	39.54772774420993
10	15.38944289253837	27.621173710696585	32.79691457007128	24.19246882669377
11	36.71619863759898	21.316317890091995	22.771093926342665	19.196389545966355
12	24.20812560914669	24.126449394017282	30.045626473531556	21.619798523304468
13	30.254383572903848	20.208731004734872	25.482326754440326	24.05455866792095
14	22.96823891206238	20.11374652452048	26.10259628595026	30.81541827746689
15	24.55427097454338	28.098575102323597	23.719764469802634	23.627389453330395
16	24.448457219799042	26.067890418179612	25.719005115853665	23.764647246167677
17	22.927400804497672	26.110163730802498	26.98616070904349	23.976274755656338
18	23.691582261387374	24.921683469438612	28.731500532982967	22.655233736191043
19	24.642731795402387	25.76519262408979	26.547509853987457	23.04456572652037
20	24.662433246655645	24.69257255287752	27.61112727528929	23.03386692517754
21	25.331891169705166	24.865188579420984	25.5004625274483	24.302457723425547
22	24.482510721634153	25.809031614957966	26.169007138188068	23.539450525219817
23	23.411064909105857	25.358768646249352	26.69781496553551	24.532351479109284
24	23.722504406731897	25.914453950140974	26.846945818399593	23.51609582472754
25	24.170288384885463	25.301621390296187	26.60987603742493	23.91821418739342
26	22.683154998232087	26.045709976371306	27.53806229050899	23.733072734887617
27	24.123578983900913	26.04440524450023	26.74504625926849	23.08696951233037
28	23.323256454182385	25.51729356858519	26.80845622820282	24.350993749029605
29	23.26036837799648	25.878704296873472	27.040828974441606	23.820098350688443
30	23.704499106911033	25.527992369928015	27.149513139302307	23.617995383858638
31	23.47108257517539	25.608755272747675	26.68515906638606	24.235003085690877
32	23.178039796931532	26.181923983711723	26.666762347003882	23.973273872352863
33	23.05852635754089	25.6193236009034	27.20131099458406	24.12083904697165
34	23.36566023999238	26.063193383443732	27.513533331332745	23.05761304523114
35	24.191425041196908	25.798332813615136	26.88974102377091	23.120501121417043
36	23.202046863359346	26.31187527807098	26.90552827941094	23.580549579158735
37	24.07439059236132	25.936373445575068	26.26086026191188	23.72837570015174
38	23.44094326895351	26.35336575157122	26.279126508106955	23.926564471368312
39	23.760602577367337	25.792852939756617	26.486317929233955	23.960226553642094
40	24.439324096701505	25.76440978496714	26.53876815045124	23.257497967880113
41	23.2148332356959	25.694737103051636	27.384495349283245	23.70593431196922
42	24.161155261787925	26.590174586171667	26.571647393602376	22.677022758438028
43	23.20452585391439	25.833951993695536	26.737348341229133	24.224173811160938
44	22.903524211256965	26.130256601617084	27.043960330932194	23.922258856193757
45	23.114107935248768	26.329228211956302	26.5839118731905	23.972751979604432
46	23.070790837129014	25.61958454727761	26.79306039212412	24.516564223469256
47	23.761124470115767	25.40456473492415	26.93866846893629	23.89564232602379
48	23.23792698981396	27.06535793361785	25.7688458733288	23.92786920323939
49	23.16681910284027	25.916932940696025	26.716733577666123	24.199514378797584
50	22.545665675069152	27.486300297479254	25.504149052763424	24.46388497468817
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	39.0
2	44.0
3	155.5
4	267.0
5	295.5
6	324.0
7	287.0
8	250.0
9	332.0
10	414.0
11	649.5
12	885.0
13	1447.0
14	2009.0
15	2740.0
16	3471.0
17	4001.0
18	4531.0
19	4876.0
20	5221.0
21	5773.5
22	6326.0
23	6700.0
24	7074.0
25	7470.0
26	7866.0
27	9612.0
28	11358.0
29	12942.5
30	14527.0
31	15998.5
32	17470.0
33	20970.5
34	24471.0
35	27329.5
36	30188.0
37	32841.5
38	35495.0
39	36091.0
40	36687.0
41	38386.0
42	40085.0
43	41022.5
44	41960.0
45	45868.5
46	49777.0
47	52662.5
48	55548.0
49	54490.5
50	53433.0
51	50675.5
52	47918.0
53	46538.0
54	45158.0
55	44993.0
56	44828.0
57	43833.0
58	42838.0
59	39292.0
60	35746.0
61	31921.0
62	28096.0
63	24609.0
64	21122.0
65	18383.0
66	15644.0
67	13519.5
68	11395.0
69	9958.5
70	8522.0
71	6866.0
72	5210.0
73	4536.0
74	3862.0
75	2984.0
76	2106.0
77	1865.0
78	1624.0
79	1378.0
80	1132.0
81	879.5
82	627.0
83	533.5
84	440.0
85	356.0
86	272.0
87	201.0
88	130.0
89	83.0
90	36.0
91	24.0
92	12.0
93	7.5
94	3.0
95	2.0
96	1.0
97	2.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01670056794978348
2	0.005479873858522704
3	1.3047318710768344E-4
4	1.3047318710768344E-4
5	0.0
6	6.523659355384172E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	1.3047318710768344E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	766441.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.15982172019284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.73872062525378	51.801819212327395
2	12.618746365587771	16.19233034296886
3	3.3438671614774726	6.43625762809206
4	1.243391254471211	3.1910304486127936
5	0.5791243823219183	1.8578258561795544
6	0.32384381573780285	1.246665688975465
7	0.2195008333316678	0.9858194033795499
8	0.13898585981937073	0.7133846390110834
9	0.10263137798283047	0.5926329822848515
>10	0.5616248380757166	6.974152943079444
>50	0.07777973768672973	3.500702445741308
>100	0.05076035402113012	5.88084313672821
>500	6.140365395614673E-4	0.288513517909205
>1k	4.093576930409782E-4	0.33802175471019286
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1308	0.17065892873684993	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1266	0.16517905487832724	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	875	0.11416403871922301	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	791	0.1032042910021776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.3047318710768344E-4	0.0
12	0.0	0.0	0.0	1.3047318710768344E-4	0.0
13	0.0	0.0	0.0	2.609463742153669E-4	0.0
14	0.0	0.0	0.0	2.609463742153669E-4	0.0
15	0.0	0.0	0.0	2.609463742153669E-4	0.0
16	0.0	0.0	0.0	2.609463742153669E-4	0.0
17	0.0	0.0	0.0	5.218927484307338E-4	0.0
18	0.0	0.0	0.0	6.523659355384172E-4	0.0
19	0.0	0.0	0.0	7.828391226461006E-4	0.0
20	0.0	0.0	0.0	9.13312309753784E-4	0.0
21	0.0	0.0	0.0	0.0013047318710768343	0.0
22	0.0	0.0	0.0	0.002739936929261352	0.0
23	0.0	0.0	0.0	0.005871293419845754	0.0
24	0.0	0.0	0.0	0.009655015845968574	0.0
25	0.0	0.0	0.0	0.012786372336552977	0.0
26	0.0	0.0	0.0	0.016570094762675798	0.0
27	0.0	0.0	0.0	0.022702334556736918	0.0
28	0.0	0.0	0.0	0.031704984467167074	0.0
29	0.0	0.0	0.0	0.05049312341067349	0.0
30	0.0	0.0	0.0	0.08376378612313276	0.0
31	0.0	0.0	0.0	0.14391192537977482	0.0
32	0.0	0.0	0.0	0.22023873983776965	0.0
33	0.0	0.0	0.0	0.29904454485081045	0.0
34	0.0	0.0	0.0	0.3867225265871737	0.0
35	0.0	0.0	0.0	0.47961943580784433	0.0
36	0.0	0.0	0.0	0.6184429068904195	0.0
37	0.0	0.0	0.0	0.8142831607390523	0.0
38	0.0	0.0	0.0	1.04026272080956	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTTT	25	0.0023522915	35.199894	24
TCGCGTT	30	0.005743802	29.333246	23
ATACCGT	105	3.0649971E-9	23.04755	6
TGTAGGA	615	0.0	22.178797	2
GGACCGT	50	0.0025796287	21.999935	6
TAGGACC	460	0.0	21.999935	4
GTCCTAA	225	0.0	21.513855	1
TACCGTC	105	7.7370714E-8	20.952318	7
TTAGGAC	370	0.0	20.81075	3
TCCTACA	595	0.0	20.705822	2
TGGAACG	65	4.929996E-4	20.307632	5
CCGCCGT	65	4.929996E-4	20.307632	24
TGGTATA	175	0.0	20.114227	44
TACGACG	135	2.593879E-9	19.555498	5
GATATAC	275	0.0	19.202448	1
CTGTAGG	600	0.0	19.0691	1
GTCCTAC	630	0.0	18.859547	1
GACGGTA	140	4.0745363E-9	18.857086	8
GCGTATG	70	8.1197824E-4	18.857086	8
ACGACGG	140	4.0745363E-9	18.857086	6
>>END_MODULE