FastQCFastQC Report
Thu 2 Feb 2017
SRR4062665_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062665_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT130.705371676614216No Hit
TCCATGTACTCTGCGTTGATACCAC110.5968529571351058No Hit
GTACATGGAAGCAGTGGTATCAACG110.5968529571351058No Hit
GGTATCAACGCAGAGTACTTTTTTT50.27129679869777534No Hit
ACTCTGCGTTGATACCACTGCTTCC40.2170374389582203No Hit
CATGTACTCTGCGTTGATACCACTG40.2170374389582203No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40.2170374389582203No Hit
TATCAACGCAGAGTACTTTTTTTTT40.2170374389582203No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40.2170374389582203No Hit
TATCAACGCAGAGTACATGGGAAGC30.16277807921866522No Hit
CAGTGGTATCAACGCAGAGTACATG30.16277807921866522No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG30.16277807921866522No Hit
GTACATGGGAAGCAGTGGTATCAAC30.16277807921866522No Hit
GCGTTGATACCACTGCCCATGTACT30.16277807921866522No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30.16277807921866522No Hit
GCGCAAGACGGACCAGAGCGAAAGC30.16277807921866522No Hit
GAGTACATGGAAGCAGTGGTATCAA30.16277807921866522No Hit
GGGGGGTCAGCGCCCGTCGGCATGT30.16277807921866522No Hit
GGGTAGGCACACGCTGAGCCAGTCA30.16277807921866522No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC30.16277807921866522No Hit
AGGTTATCTAGAGTCACCAAAGCCG30.16277807921866522No Hit
GGGCGGGGACGGGCGGTGACTCGCC30.16277807921866522No Hit
GTCCTATTCCATTATTCCTAGCTGC30.16277807921866522No Hit
GCTTTTAGGCCTACAATGGTTAAAA20.10851871947911015No Hit
ACAGTGGACATTTCTAAATTTTCCA20.10851871947911015No Hit
TATCAACGCAGAGTACATGGAAGCA20.10851871947911015No Hit
ATACCACTGCTTCCCATGTACTCTG20.10851871947911015No Hit
ACCCTATGGAGCTTAAATTATATAA20.10851871947911015No Hit
CTCCGAGGTCACCCCAACCGAAATT20.10851871947911015No Hit
GACTAATCCTAGCCCTAGCCCTACA20.10851871947911015No Hit
GGTTGATCCTGCCAGTAGCATATGC20.10851871947911015No Hit
GGACTCTTTCGAGGCCCTGTAATTG20.10851871947911015No Hit
CTCCGGGCCGCGCTGACCATCCAGC20.10851871947911015No Hit
GTCTGGTGCCAGCAGCCGCGGTAAT20.10851871947911015No Hit
CTGTACACCAACCCAGGGCCTGAGG20.10851871947911015No Hit
CCTTCAACACGCTGACAGACCGTGA20.10851871947911015No Hit
GTATCAACGCAGAGTACATGGGGAA20.10851871947911015No Hit
GTTTATGGTCGGAACTACGACGGTA20.10851871947911015No Hit
TCCAGACACCGATGTTATATTGATG20.10851871947911015No Hit
ACGCAGAGTACATGGGAAGCAGTGG20.10851871947911015No Hit
TCGTAGTTCCGACCATAAACGATGC20.10851871947911015No Hit
ACGCAGAGTACATGGGCAGTGGTAT20.10851871947911015No Hit
TACCACTGCTTCCCATGTACTCTGC20.10851871947911015No Hit
GGCATAGACAGCTAGGAGGTTGGCT20.10851871947911015No Hit
GTACATGGGAGTGGTATCAACGCAA20.10851871947911015No Hit
ACTAAGAACGGCCATGCACCACCAC20.10851871947911015No Hit
TAACTAGTTAGCATGCCAGAGTCTC20.10851871947911015No Hit
GAACTACGACGGTATCTGATCGTCT20.10851871947911015No Hit
CTCCGGAATCGAACCCTGATTCCCC20.10851871947911015No Hit
GTTCTATCAACTAGAGGCTGTTCAC20.10851871947911015No Hit
GTGTATATCAATGAGTTACAATGAA20.10851871947911015No Hit
GTGTGTGACCTGCTCAGGTTAAAGG20.10851871947911015No Hit
CTTCATACAAAGAAGCTTCTTGCTA20.10851871947911015No Hit
GTTTTAGGTAGACAGCTAATCATAG20.10851871947911015No Hit
ACGCAGAGTACATGGAAGCAGTGGT20.10851871947911015No Hit
ATTCGAACGTCTGCCCTATCAACTT20.10851871947911015No Hit
AGTCGGCATCGTTTATGGTCGGAAC20.10851871947911015No Hit
GAATAATGGAATAGGACCGCGGTTC20.10851871947911015No Hit
ATATTAAAGTTGCTGCAGTTAAAAA20.10851871947911015No Hit
AAAAAGTACTCTGCGTTGATACCAC20.10851871947911015No Hit
GCGTTGATACCACTGCTTCCCATGT20.10851871947911015No Hit
CCTATAGTCTGATTAACTAACAATG20.10851871947911015No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG20.10851871947911015No Hit
GCGTTGATACCACTGCTTCCATGTA20.10851871947911015No Hit
TATCTACATTGTTCTATCAACTAGA20.10851871947911015No Hit
GAATAGGACCGCGGTTCTATTTTGT20.10851871947911015No Hit
AGGATCCATTGGAGGGCAAGTCTGG20.10851871947911015No Hit
GTATCAACGCAGAGTACATGGGAGT20.10851871947911015No Hit
AGCTAATACATGCCGACGGGCGCTG20.10851871947911015No Hit
GTCATAGAGGGTGAGAATCCCGTAT20.10851871947911015No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)