##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062665_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1843
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.863266413456323	32.0	32.0	32.0	14.0	32.0
2	27.99403147042865	32.0	32.0	32.0	14.0	32.0
3	27.93163320672816	32.0	21.0	32.0	14.0	32.0
4	27.702116115029842	32.0	21.0	32.0	14.0	32.0
5	27.738469886055345	32.0	21.0	32.0	14.0	32.0
6	31.04286489419425	36.0	32.0	36.0	14.0	36.0
7	30.88171459576777	36.0	32.0	36.0	14.0	36.0
8	30.790016277807922	36.0	27.0	36.0	14.0	36.0
9	30.95387954422138	36.0	32.0	36.0	14.0	36.0
10	29.98155181768855	36.0	21.0	36.0	14.0	36.0
11	31.557786218122626	36.0	32.0	36.0	14.0	36.0
12	30.55832881172002	36.0	27.0	36.0	14.0	36.0
13	30.667932718393924	36.0	27.0	36.0	14.0	36.0
14	30.50135648399349	36.0	27.0	36.0	14.0	36.0
15	30.295713510580576	36.0	27.0	36.0	14.0	36.0
16	30.714053174172545	36.0	27.0	36.0	14.0	36.0
17	30.46228974498101	36.0	27.0	36.0	14.0	36.0
18	30.557786218122626	36.0	27.0	36.0	14.0	36.0
19	30.500813890396092	36.0	27.0	36.0	14.0	36.0
20	30.56809549647314	36.0	27.0	36.0	14.0	36.0
21	30.252306022788932	36.0	27.0	36.0	14.0	36.0
22	30.10634834508953	36.0	21.0	36.0	14.0	36.0
23	30.12425393380358	36.0	21.0	36.0	14.0	36.0
24	30.25718936516549	36.0	27.0	36.0	14.0	36.0
25	29.42322300596853	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	0.0
9	0.0
10	0.0
11	1.0
12	0.0
13	0.0
14	7.0
15	1.0
16	9.0
17	14.0
18	36.0
19	35.0
20	41.0
21	60.0
22	43.0
23	48.0
24	58.0
25	65.0
26	51.0
27	81.0
28	74.0
29	91.0
30	97.0
31	134.0
32	146.0
33	178.0
34	282.0
35	289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.671188279978296	20.835594139989148	12.533912099837222	24.959305480195333
2	17.145957677699403	18.39392295170917	38.90396093326099	25.55615843733044
3	19.37059142702116	24.796527400976668	29.9511665762344	25.881714595767768
4	12.805208898534998	15.843733043950081	37.05914270211611	34.29191535539881
5	14.169381107491857	36.047774158523346	33.930510314875136	15.852334419109662
6	30.798479087452474	37.26235741444867	17.43617599130907	14.502987506789788
7	28.19120043454644	32.21075502444324	21.347093970668116	18.250950570342205
8	28.082563824008687	32.10211841390549	20.912547528517113	18.902770233568713
9	25.43478260869565	15.543478260869565	19.619565217391305	39.40217391304348
10	15.489130434782608	28.097826086956523	32.119565217391305	24.293478260869566
11	36.30434782608696	21.57608695652174	22.880434782608695	19.23913043478261
12	24.130434782608695	25.59782608695652	29.184782608695652	21.086956521739133
13	28.369565217391308	21.90217391304348	24.67391304347826	25.05434782608696
14	22.228260869565215	20.48913043478261	26.14130434782609	31.141304347826086
15	23.641304347826086	27.500000000000004	23.369565217391305	25.48913043478261
16	24.347826086956523	27.336956521739133	26.14130434782609	22.17391304347826
17	22.5	26.14130434782609	26.6304347826087	24.728260869565215
18	24.63295269168026	26.916802610114193	26.916802610114193	21.533442088091352
19	22.94725394235998	28.167482327351824	26.590538336052198	22.294725394235996
20	24.469820554649267	24.57857531266993	28.167482327351824	22.784121805328983
21	23.762914627514952	25.502990755845566	27.569331158238175	23.164763458401303
22	23.491027732463294	27.7868406742795	26.80804785209353	21.914083741163676
23	22.838499184339316	26.373028820010873	27.623708537248504	23.164763458401303
24	23.382272974442632	26.373028820010873	27.351821642196843	22.892876563349645
25	22.39130434782609	26.684782608695652	26.032608695652176	24.891304347826086
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	2.0
3	4.0
4	4.0
5	4.0
6	4.5
7	5.0
8	5.0
9	5.0
10	7.0
11	9.0
12	9.0
13	9.0
14	10.5
15	12.0
16	12.0
17	12.0
18	17.5
19	23.0
20	23.0
21	23.0
22	32.0
23	41.0
24	41.0
25	41.0
26	62.0
27	83.0
28	83.0
29	83.0
30	102.0
31	121.0
32	121.0
33	121.0
34	126.5
35	132.0
36	132.0
37	132.0
38	134.0
39	136.0
40	136.0
41	136.0
42	165.5
43	195.0
44	195.0
45	195.0
46	240.0
47	285.0
48	285.0
49	285.0
50	271.5
51	258.0
52	258.0
53	258.0
54	202.0
55	146.0
56	146.0
57	146.0
58	141.0
59	136.0
60	136.0
61	136.0
62	115.5
63	95.0
64	95.0
65	95.0
66	81.5
67	68.0
68	68.0
69	68.0
70	57.0
71	46.0
72	46.0
73	46.0
74	35.5
75	25.0
76	25.0
77	25.0
78	19.0
79	13.0
80	13.0
81	13.0
82	9.5
83	6.0
84	6.0
85	6.0
86	3.5
87	1.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	1.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.05425935973955508
6	0.10851871947911015
7	0.10851871947911015
8	0.10851871947911015
9	0.16277807921866522
10	0.16277807921866522
11	0.16277807921866522
12	0.16277807921866522
13	0.16277807921866522
14	0.16277807921866522
15	0.16277807921866522
16	0.16277807921866522
17	0.16277807921866522
18	0.2170374389582203
19	0.2170374389582203
20	0.2170374389582203
21	0.2170374389582203
22	0.2170374389582203
23	0.2170374389582203
24	0.2170374389582203
25	0.16277807921866522
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1843.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.16332067281607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.9231217239371	89.36516549104721
2	2.7373325567850904	5.100379815518177
3	0.8153756552125802	2.278893109061313
4	0.29120559114735	1.0851871947911014
5	0.05824111822947001	0.27129679869777534
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.17472335468841002	1.8990775908844275
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	13	0.705371676614216	No Hit
TCCATGTACTCTGCGTTGATACCAC	11	0.5968529571351058	No Hit
GTACATGGAAGCAGTGGTATCAACG	11	0.5968529571351058	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5	0.27129679869777534	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4	0.2170374389582203	No Hit
CATGTACTCTGCGTTGATACCACTG	4	0.2170374389582203	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4	0.2170374389582203	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4	0.2170374389582203	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4	0.2170374389582203	No Hit
TATCAACGCAGAGTACATGGGAAGC	3	0.16277807921866522	No Hit
CAGTGGTATCAACGCAGAGTACATG	3	0.16277807921866522	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	3	0.16277807921866522	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3	0.16277807921866522	No Hit
GCGTTGATACCACTGCCCATGTACT	3	0.16277807921866522	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3	0.16277807921866522	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3	0.16277807921866522	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3	0.16277807921866522	No Hit
GGGGGGTCAGCGCCCGTCGGCATGT	3	0.16277807921866522	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	3	0.16277807921866522	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	3	0.16277807921866522	No Hit
AGGTTATCTAGAGTCACCAAAGCCG	3	0.16277807921866522	No Hit
GGGCGGGGACGGGCGGTGACTCGCC	3	0.16277807921866522	No Hit
GTCCTATTCCATTATTCCTAGCTGC	3	0.16277807921866522	No Hit
GCTTTTAGGCCTACAATGGTTAAAA	2	0.10851871947911015	No Hit
ACAGTGGACATTTCTAAATTTTCCA	2	0.10851871947911015	No Hit
TATCAACGCAGAGTACATGGAAGCA	2	0.10851871947911015	No Hit
ATACCACTGCTTCCCATGTACTCTG	2	0.10851871947911015	No Hit
ACCCTATGGAGCTTAAATTATATAA	2	0.10851871947911015	No Hit
CTCCGAGGTCACCCCAACCGAAATT	2	0.10851871947911015	No Hit
GACTAATCCTAGCCCTAGCCCTACA	2	0.10851871947911015	No Hit
GGTTGATCCTGCCAGTAGCATATGC	2	0.10851871947911015	No Hit
GGACTCTTTCGAGGCCCTGTAATTG	2	0.10851871947911015	No Hit
CTCCGGGCCGCGCTGACCATCCAGC	2	0.10851871947911015	No Hit
GTCTGGTGCCAGCAGCCGCGGTAAT	2	0.10851871947911015	No Hit
CTGTACACCAACCCAGGGCCTGAGG	2	0.10851871947911015	No Hit
CCTTCAACACGCTGACAGACCGTGA	2	0.10851871947911015	No Hit
GTATCAACGCAGAGTACATGGGGAA	2	0.10851871947911015	No Hit
GTTTATGGTCGGAACTACGACGGTA	2	0.10851871947911015	No Hit
TCCAGACACCGATGTTATATTGATG	2	0.10851871947911015	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2	0.10851871947911015	No Hit
TCGTAGTTCCGACCATAAACGATGC	2	0.10851871947911015	No Hit
ACGCAGAGTACATGGGCAGTGGTAT	2	0.10851871947911015	No Hit
TACCACTGCTTCCCATGTACTCTGC	2	0.10851871947911015	No Hit
GGCATAGACAGCTAGGAGGTTGGCT	2	0.10851871947911015	No Hit
GTACATGGGAGTGGTATCAACGCAA	2	0.10851871947911015	No Hit
ACTAAGAACGGCCATGCACCACCAC	2	0.10851871947911015	No Hit
TAACTAGTTAGCATGCCAGAGTCTC	2	0.10851871947911015	No Hit
GAACTACGACGGTATCTGATCGTCT	2	0.10851871947911015	No Hit
CTCCGGAATCGAACCCTGATTCCCC	2	0.10851871947911015	No Hit
GTTCTATCAACTAGAGGCTGTTCAC	2	0.10851871947911015	No Hit
GTGTATATCAATGAGTTACAATGAA	2	0.10851871947911015	No Hit
GTGTGTGACCTGCTCAGGTTAAAGG	2	0.10851871947911015	No Hit
CTTCATACAAAGAAGCTTCTTGCTA	2	0.10851871947911015	No Hit
GTTTTAGGTAGACAGCTAATCATAG	2	0.10851871947911015	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2	0.10851871947911015	No Hit
ATTCGAACGTCTGCCCTATCAACTT	2	0.10851871947911015	No Hit
AGTCGGCATCGTTTATGGTCGGAAC	2	0.10851871947911015	No Hit
GAATAATGGAATAGGACCGCGGTTC	2	0.10851871947911015	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	2	0.10851871947911015	No Hit
AAAAAGTACTCTGCGTTGATACCAC	2	0.10851871947911015	No Hit
GCGTTGATACCACTGCTTCCCATGT	2	0.10851871947911015	No Hit
CCTATAGTCTGATTAACTAACAATG	2	0.10851871947911015	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	2	0.10851871947911015	No Hit
GCGTTGATACCACTGCTTCCATGTA	2	0.10851871947911015	No Hit
TATCTACATTGTTCTATCAACTAGA	2	0.10851871947911015	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2	0.10851871947911015	No Hit
AGGATCCATTGGAGGGCAAGTCTGG	2	0.10851871947911015	No Hit
GTATCAACGCAGAGTACATGGGAGT	2	0.10851871947911015	No Hit
AGCTAATACATGCCGACGGGCGCTG	2	0.10851871947911015	No Hit
GTCATAGAGGGTGAGAATCCCGTAT	2	0.10851871947911015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE