##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062665_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1843
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.442213781877374	32.0	32.0	32.0	21.0	32.0
2	29.598480737927293	32.0	32.0	32.0	14.0	32.0
3	30.62072707542051	32.0	32.0	32.0	32.0	32.0
4	31.036896364622898	32.0	32.0	32.0	32.0	32.0
5	30.837221920781335	32.0	32.0	32.0	32.0	32.0
6	34.431361909929464	36.0	36.0	36.0	32.0	36.0
7	34.366250678242	36.0	36.0	36.0	32.0	36.0
8	34.33478024959305	36.0	36.0	36.0	32.0	36.0
9	34.633749321758	36.0	36.0	36.0	32.0	36.0
10	34.18176885512751	36.0	36.0	36.0	32.0	36.0
11	34.78350515463917	36.0	36.0	36.0	32.0	36.0
12	34.40477482365708	36.0	36.0	36.0	32.0	36.0
13	34.608247422680414	36.0	36.0	36.0	32.0	36.0
14	34.76451437873033	36.0	36.0	36.0	32.0	36.0
15	34.55398806294086	36.0	36.0	36.0	32.0	36.0
16	34.70971242539338	36.0	36.0	36.0	32.0	36.0
17	34.36353771025502	36.0	36.0	36.0	32.0	36.0
18	34.540423223005966	36.0	36.0	36.0	32.0	36.0
19	34.53065653825285	36.0	36.0	36.0	32.0	36.0
20	34.41671188279978	36.0	36.0	36.0	32.0	36.0
21	34.60119370591427	36.0	36.0	36.0	32.0	36.0
22	34.46717308735757	36.0	36.0	36.0	32.0	36.0
23	34.23928377645144	36.0	36.0	36.0	32.0	36.0
24	34.60607704829083	36.0	36.0	36.0	32.0	36.0
25	34.26098752034726	36.0	36.0	36.0	32.0	36.0
26	34.13998914812805	36.0	36.0	36.0	32.0	36.0
27	34.37330439500814	36.0	36.0	36.0	32.0	36.0
28	34.196961475854586	36.0	36.0	36.0	32.0	36.0
29	34.06619641888226	36.0	36.0	36.0	32.0	36.0
30	34.24742268041237	36.0	36.0	36.0	32.0	36.0
31	34.19641888225719	36.0	36.0	36.0	32.0	36.0
32	33.90667390124796	36.0	36.0	36.0	32.0	36.0
33	33.79761258817146	36.0	36.0	36.0	32.0	36.0
34	33.95496473141617	36.0	36.0	36.0	32.0	36.0
35	33.74118285404232	36.0	36.0	36.0	32.0	36.0
36	33.721106890938685	36.0	36.0	36.0	32.0	36.0
37	33.71839392295171	36.0	36.0	36.0	32.0	36.0
38	33.569723277265325	36.0	36.0	36.0	27.0	36.0
39	33.75149213239284	36.0	36.0	36.0	32.0	36.0
40	33.403147042864894	36.0	36.0	36.0	21.0	36.0
41	33.61096039066739	36.0	36.0	36.0	27.0	36.0
42	33.47314161692892	36.0	36.0	36.0	21.0	36.0
43	33.50244167118828	36.0	36.0	36.0	21.0	36.0
44	33.323928377645146	36.0	36.0	36.0	21.0	36.0
45	33.255561584373304	36.0	36.0	36.0	21.0	36.0
46	33.35756918068367	36.0	36.0	36.0	21.0	36.0
47	33.18665219750407	36.0	36.0	36.0	21.0	36.0
48	33.10526315789474	36.0	36.0	36.0	14.0	36.0
49	33.21758003255562	36.0	36.0	36.0	21.0	36.0
50	32.56592512208356	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	3.0
25	6.0
26	12.0
27	26.0
28	41.0
29	51.0
30	77.0
31	123.0
32	172.0
33	255.0
34	439.0
35	638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91146116241173	20.0434546442151	11.569799022270505	25.475285171102662
2	18.61096039066739	21.106890938686924	36.67932718393923	23.602821486706457
3	22.734671730873576	26.478567552902877	28.811720021703746	21.975040694519805
4	14.053174172544763	15.843733043950081	36.24525230602279	33.85784047748236
5	16.549104720564298	34.780249593054805	32.82691264243082	15.843733043950081
6	33.36950623982637	35.919696147585455	16.49484536082474	14.215952251763428
7	27.238198589256648	31.795984807379273	21.758003255561583	19.207813347802496
8	26.96690179055887	31.958762886597935	19.967444384156266	21.106890938686924
9	25.718936516549107	15.897992403689637	18.7194791101465	39.66359196961476
10	15.19262072707542	26.26153011394465	32.772653282691266	25.773195876288657
11	39.0124796527401	20.835594139989148	21.70374389582203	18.448182311448726
12	23.98263700488334	22.89744981009224	29.9511665762344	23.168746608790016
13	30.71079761258817	20.130222463374935	26.207270754205105	22.951709169831798
14	23.494302767227346	18.990775908844277	26.804123711340207	30.71079761258817
15	25.122083559413998	27.2924579489962	23.114487249050462	24.47097124253934
16	25.447639717851327	27.88931090613131	22.4633749321758	24.199674443841563
17	22.843190450352687	27.129679869777533	25.773195876288657	24.25393380358112
18	22.843190450352687	25.773195876288657	28.32338578404775	23.060227889310905
19	25.393380358111774	26.804123711340207	26.09875203472599	21.70374389582203
20	25.827455236028214	22.951709169831798	26.478567552902877	24.742268041237114
21	25.447639717851327	23.657080846446014	26.641345632121542	24.25393380358112
22	25.122083559413998	27.129679869777533	25.66467715680955	22.083559413998916
23	21.3781877373847	26.478567552902877	27.238198589256648	24.90504612045578
24	22.951709169831798	27.2924579489962	25.773195876288657	23.98263700488334
25	23.657080846446014	28.106348345089526	25.610417797069996	22.626153011394464
26	23.33152468800868	26.26153011394465	26.74986435160065	23.657080846446014
27	23.711340206185564	27.88931090613131	25.122083559413998	23.277265328269124
28	23.060227889310905	25.55615843733044	29.137276180141075	22.24633749321758
29	24.579489962018446	25.881714595767768	25.339120998372216	24.199674443841563
30	24.036896364622898	27.02116115029843	27.34671730873576	21.595225176342918
31	23.168746608790016	26.31578947368421	26.42430819316332	24.091155724362455
32	22.02930005425936	27.726532826912642	26.044492674986436	24.199674443841563
33	21.486706456863807	27.509495387954424	27.997829625610414	23.00596852957135
34	23.98263700488334	26.478567552902877	26.912642430819318	22.626153011394464
35	23.657080846446014	25.013564839934887	28.486163863266412	22.843190450352687
36	24.036896364622898	25.501899077590885	26.695604991861092	23.765599565925122
37	23.711340206185564	27.835051546391753	25.881714595767768	22.57189365165491
38	22.843190450352687	27.2924579489962	25.99023331524688	23.874118285404233
39	25.935973955507325	27.2924579489962	24.90504612045578	21.866521975040694
40	23.060227889310905	28.811720021703746	25.067824199674444	23.060227889310905
41	22.02930005425936	27.02116115029843	27.563754747693974	23.38578404774824
42	22.734671730873576	30.16820401519262	25.176342919153555	21.920781334780248
43	23.114487249050462	26.53282691264243	27.672273467173085	22.68041237113402
44	24.579489962018446	25.827455236028214	26.53282691264243	23.060227889310905
45	23.98263700488334	27.075420510037983	26.96690179055887	21.975040694519805
46	23.765599565925122	26.370048833423766	26.53282691264243	23.33152468800868
47	21.540965816603364	26.478567552902877	27.129679869777533	24.850786760716222
48	23.602821486706457	27.075420510037983	25.393380358111774	23.928377645143787
49	24.25393380358112	27.726532826912642	26.26153011394465	21.758003255561583
50	22.734671730873576	27.400976668475312	25.935973955507325	23.928377645143787
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	2.0
4	1.0
5	1.0
6	1.0
7	2.0
8	3.0
9	3.0
10	3.0
11	3.0
12	3.0
13	4.0
14	5.0
15	7.0
16	9.0
17	8.0
18	7.0
19	9.0
20	11.0
21	15.5
22	20.0
23	23.0
24	26.0
25	26.0
26	26.0
27	25.0
28	24.0
29	34.5
30	45.0
31	45.5
32	46.0
33	50.5
34	55.0
35	56.0
36	57.0
37	71.0
38	85.0
39	86.5
40	88.0
41	94.5
42	101.0
43	102.0
44	103.0
45	108.0
46	113.0
47	122.0
48	131.0
49	137.5
50	144.0
51	119.5
52	95.0
53	95.5
54	96.0
55	98.5
56	101.0
57	106.5
58	112.0
59	98.0
60	84.0
61	71.0
62	58.0
63	53.0
64	48.0
65	43.5
66	39.0
67	33.5
68	28.0
69	24.0
70	20.0
71	15.5
72	11.0
73	11.5
74	12.0
75	11.5
76	11.0
77	7.5
78	4.0
79	3.0
80	2.0
81	2.5
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	4.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10851871947911015
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1843.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.36462289744982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.12837837837837	93.5973955507325
2	2.2522522522522523	4.3407487791644055
3	0.5630630630630631	1.6277807921866523
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.05630630630630631	0.4340748779164406
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	8	0.4340748779164406	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.16277807921866522	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3	0.16277807921866522	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	3	0.16277807921866522	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	3	0.16277807921866522	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3	0.16277807921866522	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	3	0.16277807921866522	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.16277807921866522	No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT	3	0.16277807921866522	No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT	3	0.16277807921866522	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT	3	0.16277807921866522	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2	0.10851871947911015	No Hit
CCGCAGCTTCGGCATATAGTTTTAGCCCCGTTACATCTTCCGCGCAGGCC	2	0.10851871947911015	No Hit
GTTGGTAACTAAAATTATGAAATAAAAAATAGTGTATGCAATAGTATCCA	2	0.10851871947911015	No Hit
TCCTACGCCGCCGCCGTCGCAGGATGGAAGGCATTATGGTTGATGGGAAT	2	0.10851871947911015	No Hit
AATCCACCTTCCTCTGCCTCCCGAGTGCTAGGATTAAAGGCATGCGCCAC	2	0.10851871947911015	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2	0.10851871947911015	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC	2	0.10851871947911015	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	2	0.10851871947911015	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	2	0.10851871947911015	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	2	0.10851871947911015	No Hit
TGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAA	2	0.10851871947911015	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2	0.10851871947911015	No Hit
GTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTA	2	0.10851871947911015	No Hit
GTGTTGGGGACAGCTGACTGGTCTGGAGGACTCTTCTCTTTCTGCTCTCT	2	0.10851871947911015	No Hit
CTTTTGTGCCGTCTGGTGCGCTCCCGACGATCCTTGAAAATCCACCGGAA	2	0.10851871947911015	No Hit
CCTTGCTGGCCTCCTTTTTCCATTCTTTTGGACAATACTTATAAAGCTTT	2	0.10851871947911015	No Hit
AACCTTATGTGTATGTATGTAAACTCTCGCTCTTCCTGCCCCCACAACAC	2	0.10851871947911015	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2	0.10851871947911015	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGTTATA	2	0.10851871947911015	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2	0.10851871947911015	No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG	2	0.10851871947911015	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG	2	0.10851871947911015	No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT	2	0.10851871947911015	No Hit
GGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAAC	2	0.10851871947911015	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2	0.10851871947911015	No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCCTGTCTCTTATACA	2	0.10851871947911015	No Hit
GCTCAACACCATGGCATGGTAGCCCAGCACCTCGGCTTGCCTCTGAGCCT	2	0.10851871947911015	No Hit
ATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGA	2	0.10851871947911015	No Hit
GACTATAAATAGAGAAGGGAGTTATTATAAACATAGCTAGCTCATTCTTT	2	0.10851871947911015	No Hit
GCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTT	2	0.10851871947911015	No Hit
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAG	2	0.10851871947911015	No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA	2	0.10851871947911015	No Hit
CCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGC	2	0.10851871947911015	No Hit
ACTCATCGCTGATGTGCTCAGCAAATACAGTCTTGAAGGTCCGGAGGCCT	2	0.10851871947911015	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT	2	0.10851871947911015	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.10851871947911015	No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT	2	0.10851871947911015	No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA	2	0.10851871947911015	No Hit
GAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTG	2	0.10851871947911015	No Hit
CCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT	2	0.10851871947911015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.05425935973955507	0.0
29	0.0	0.0	0.0	0.05425935973955507	0.0
30	0.0	0.0	0.0	0.05425935973955507	0.0
31	0.0	0.0	0.0	0.16277807921866522	0.0
32	0.0	0.0	0.0	0.27129679869777534	0.0
33	0.0	0.0	0.0	0.27129679869777534	0.0
34	0.0	0.0	0.0	0.43407487791644056	0.0
35	0.0	0.0	0.0	0.48833423765599565	0.0
36	0.0	0.0	0.0	0.5425935973955507	0.0
37	0.0	0.0	0.0	0.8138903960933261	0.0
38	0.0	0.0	0.0	0.9224091155724362	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE