FastQCFastQC Report
Thu 2 Feb 2017
SRR4062664_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062664_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304963
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG149671.1469290700196098No Hit
TCCATGTACTCTGCGTTGATACCAC132921.018572940382218No Hit
GAGTACATGGAAGCAGTGGTATCAA64930.4975619998421411No Hit
GTATCAACGCAGAGTACTTTTTTTT63470.48637394316926996No Hit
CATGTACTCTGCGTTGATACCACTG60050.46016630356569493No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55500.4252994146194183No Hit
GTCCTAAAGTGTGTATTTCTCATTT49840.38192653738075333No Hit
GTCCTACAGTGGACATTTCTAAATT45120.3457569295068136No Hit
TATCAACGCAGAGTACTTTTTTTTT44560.3414656200980411No Hit
CTGTAGGACGTGGAATATGGCAAGA43400.33257647917986943No Hit
CCCATGTACTCTGCGTTGATACCAC43200.3310438686767364No Hit
GAGTACTTTTTTTTTTTTTTTTTTT41420.3174036351988524No Hit
CTTTAGGACGTGAAATATGGCGAGG40260.3085144942806808No Hit
GCTTCCATGTACTCTGCGTTGATAC39850.305372642749258No Hit
GTACATGGGAAGCAGTGGTATCAAC38600.2957938271046765No Hit
GCGTTGATACCACTGCTTCCATGTA36540.2800079389224062No Hit
CATGGAAGCAGTGGTATCAACGCAG34170.2618465044602797No Hit
CCATAGGGTCTTCTCGTCTTATTAT33640.2577850866269772No Hit
TTGTAGAACAGTGTATATCAATGAG33540.2570187813754106No Hit
GATATACACTGTTCTACAAATCCCG32280.24736333520567252No Hit
GTATTAGAGGCACTGCCTGCCCAGT32100.24598398575285274No Hit
GTATCAACGCAGAGTACATGGAAGC31290.23977691321516395No Hit
GTCCTACAGTGTGCATTTCTCATTT30880.23663506168374124No Hit
GTGTATATCAATGAGTTACAATGAA28470.2181671051209881No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28310.21694101671848168No Hit
CTGTAGGACCTGGAATATGGCGAGA27360.20966111682859975No Hit
GTACATGGGGTGGTATCAACGCAAA27030.2071323094984302No Hit
ACTCTGCGTTGATACCACTGCTTCC24950.19119316026584662No Hit
CTCTAATACTTGTAATGCTAGAGGT24600.1885110918853638No Hit
ACGCAGAGTACATGGAAGCAGTGGT23320.17870238466531235No Hit
CAGTGGTATCAACGCAGAGTACATG23170.17755292678796256No Hit
GGTATCAACGCAGAGTACTTTTTTT21840.16736106694212788No Hit
GTATCAACGCAGAGTACATGGGAAG21660.16598171748930812No Hit
CCATTGGGATGTCCTGATCCAACAT21610.16559856486352487No Hit
GATATACACTGTTCTACAATGCCGG21250.16283986595788538No Hit
CTGAAGGACCTGGAATATGGCGAGA20980.1607708417786558No Hit
CTTCTACACCATTGGGATGTCCTGA20980.1607708417786558No Hit
ATCTATAACTTTATAGATGCAACAC20030.15349094188877385No Hit
GTGTATATCAATGAGTTACAATGAG19630.15042572088250777No Hit
GTCAGGATACCGCGGCCGTTAAACT19580.15004256825672452No Hit
GATATATTTTGATCAACGGACCAAG19510.14950615458062796No Hit
TATCAACGCAGAGTACATGGAAGCA18920.1449849535963855No Hit
CTGTTAGTATGAGTAACAAGAATTC18880.14467843149575887No Hit
GTTCTACAGTGTGGTTTTTATCATT18480.1416132104894928No Hit
ATTTAGAAATGTCCACTGTAGGACG18040.13824146738260013No Hit
ATCCTGACCGTGCAAAGGTAGCATA17920.1373219010807203No Hit
GTTATATAATTTAAGCTCCATAGGG17230.1320343948449113No Hit
CCTCTAGCATTACAAGTATTAGAGG17050.13065504539209158No Hit
GTATCCTGACCGTGCAAAGGTAGCA16760.12843276016254868No Hit
GTCCTTCAGTGTGCATTTCTCATTT16590.12713004123488558No Hit
GGTCAGGATACCGCGGCCGTTAAAC16520.12659362755878903No Hit
GTTCATGCTAGTCCCTAATTAAGGA15840.1213827518481367No Hit
GTTATAGATTAACCCAATTTTAAGT15820.12122949079782339No Hit
ATCGTAAATAGATAGAAACCGACCT15670.1200800329204736No Hit
TTTCTAAATTTTCCACCTTTTTCAG15050.11532894036076118No Hit
GTACATGGGTGGTATCAACGCAAAA15010.11502241826013458No Hit
GTACATGGGAGAAATCGTAAATAGA14730.11287676355574833No Hit
GTTCTACAAATCCCGTTTCCAACGA14700.11264687198027837No Hit
ACCTATAACTTCTCTGTTAACCCAA14510.11119089200230198No Hit
GTATCAACGCAGAGTACATGGGAGA14480.11096100042683203No Hit
GCCTAAAGGAAAGATCCAAAAAGAT14430.11057784780104878No Hit
ATAAATAATCCACCTATAACTTCTC14120.10820230152119256No Hit
CTATAGAACTAGTACCGCAAGGGAA14120.10820230152119256No Hit
ATACCACTGCTTCCATGTACTCTGC13870.10628653839227625No Hit
CACTATAAATAATCCACCTATAACT13830.10598001629164966No Hit
GACTATAGGCAATAATCACACTATA13770.10552023314070974No Hit
GTATCAACGCAGAGTACATGGGCAG13650.1046006668388299No Hit
GCGTTGATACCACTGCTTCCCATGT13560.10391099211242004No Hit
GAGTACATGGGAAGCAGTGGTATCA13530.1036811005369501No Hit
TGCTAGAGGTGATGTTTTTGGTAAA13400.10268490370991361No Hit
CTGTAGAACATATTAGATGAGTGAG13230.10138218478225054No Hit
CCTATAACTTCTCTGTTAACCCAAC13160.10084577110615396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGG453.5348705E-516.89073213
CTAATGT350.002171704316.283744
CTTGCTA1100.015.5477479
AACTTGC953.0377123E-1015.0004847
TAACTTT3250.014.90817456
AATACCG456.761602E-414.7776885
TGCGGCG400.005274679514.251554518
TCGAGTT400.005276022514.25100812
TTAACCG400.00528274314.2482734
TATTAAC400.00529485814.2433542
GGTGTGC551.9579566E-413.819698
TGCCGTC551.9592319E-413.8186287
ACTTTAT3500.013.3014518
GACGGTT500.001498088613.3014518
TAAGACC500.001500690213.29838854
CGGTTGT2300.013.21933910
TAGGACC8050.012.97979454
TTGCGGT905.393067E-712.66853417
CCGGTGT1950.012.66804813
TAGAAAT5100.012.66513254
Produced by FastQC (version 0.11.5)