##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062664_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1304963
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00353649873598	32.0	32.0	32.0	32.0	32.0
2	30.85370772964444	32.0	32.0	32.0	32.0	32.0
3	30.87391903065451	32.0	32.0	32.0	32.0	32.0
4	30.859975340297005	32.0	32.0	32.0	32.0	32.0
5	30.814592444383482	32.0	32.0	32.0	32.0	32.0
6	34.38108206899353	36.0	36.0	36.0	32.0	36.0
7	34.1805537781531	36.0	36.0	36.0	32.0	36.0
8	34.163783187722565	36.0	36.0	36.0	32.0	36.0
9	34.25447081641395	36.0	36.0	36.0	32.0	36.0
10	34.07458985427173	36.0	36.0	36.0	32.0	36.0
11	34.36685407938769	36.0	36.0	36.0	32.0	36.0
12	34.18620834460441	36.0	36.0	36.0	32.0	36.0
13	34.277479897897486	36.0	36.0	36.0	32.0	36.0
14	34.21150484726387	36.0	36.0	36.0	32.0	36.0
15	34.19908074022022	36.0	36.0	36.0	32.0	36.0
16	34.19930756657468	36.0	36.0	36.0	32.0	36.0
17	34.143497555103096	36.0	36.0	36.0	32.0	36.0
18	34.19422236492529	36.0	36.0	36.0	32.0	36.0
19	34.122371285622656	36.0	36.0	36.0	32.0	36.0
20	34.06109215357064	36.0	36.0	36.0	32.0	36.0
21	33.99166796299972	36.0	36.0	36.0	32.0	36.0
22	33.95261168324313	36.0	36.0	36.0	32.0	36.0
23	33.947508090267696	36.0	36.0	36.0	32.0	36.0
24	33.91505199764285	36.0	36.0	36.0	32.0	36.0
25	33.46607911488678	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	56.0
5	137.0
6	204.0
7	64.0
8	203.0
9	213.0
10	158.0
11	34.0
12	101.0
13	76.0
14	432.0
15	444.0
16	652.0
17	931.0
18	1290.0
19	1864.0
20	2803.0
21	3787.0
22	5057.0
23	6850.0
24	8557.0
25	10876.0
26	14604.0
27	18265.0
28	24446.0
29	32236.0
30	42118.0
31	58070.0
32	84594.0
33	123937.0
34	284943.0
35	576954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.37887330527926	20.523565345274324	12.763045259226846	23.33451609021957
2	12.087291442514307	22.288331202928006	44.01264142672785	21.611735927829837
3	18.629444969813825	28.127936044239338	30.836415301685488	22.406203684261346
4	9.828650085801417	18.42343056983024	40.609477473583205	31.138441870785133
5	9.859286651703034	41.17705228686021	37.13533809225938	11.828322969177368
6	27.49611488442021	40.49221941875451	19.980189168903433	12.031476527921848
7	24.931389621832018	34.410395497901625	23.087440179188302	17.570774701078058
8	24.179408750928044	38.45243500616651	21.649450535965197	15.718705706940241
9	25.829781946374826	16.110410549536862	21.594350303286912	36.465457200801396
10	14.58169659845574	29.050995543989295	35.67677964229159	20.69052821526337
11	32.68868847365171	23.362500527464604	26.445118746667383	17.50369225221631
12	22.776032546726068	26.921796085737483	33.418787416534556	16.883383951001893
13	28.04227486333557	23.275879927112307	28.21774239953966	20.46410281001247
14	20.063912716558995	24.8328141544033	27.840164500437336	27.263108628600364
15	22.830357314102688	32.68271351579432	26.43832831469725	18.04860085540574
16	21.232467382023305	28.70596755348489	31.24375239259869	18.817812671893112
17	18.73594031481419	29.492117369407968	31.496226710409616	20.27571560536823
18	19.33917601869741	27.8951907568738	35.02242799332762	17.743205231101175
19	21.98140229375291	29.396314987044214	28.12291151779473	20.499371201408138
20	20.837310075402097	29.44333794686561	31.895747468950724	17.823604508781568
21	21.627992090913068	27.812164556272446	27.203560777128406	23.35628257568608
22	20.37042437488776	31.62881433195758	28.32401925948892	19.676742033665732
23	19.757116460094416	30.671068221023102	28.974750916223137	20.59706440265935
24	20.895974758507762	31.284687293545144	28.920906256737954	18.89843169120914
25	21.594088137757257	29.640030753223794	29.43032875564538	19.33555235337357
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	509.0
1	509.0
2	1196.0
3	1883.0
4	1883.0
5	1883.0
6	5795.0
7	9707.0
8	9707.0
9	9707.0
10	9403.0
11	9099.0
12	9099.0
13	9099.0
14	8501.5
15	7904.0
16	7904.0
17	7904.0
18	13508.0
19	19112.0
20	19112.0
21	19112.0
22	31499.0
23	43886.0
24	43886.0
25	43886.0
26	69958.5
27	96031.0
28	96031.0
29	96031.0
30	122360.0
31	148689.0
32	148689.0
33	148689.0
34	150020.0
35	151351.0
36	151351.0
37	151351.0
38	147191.0
39	143031.0
40	143031.0
41	143031.0
42	143605.5
43	144180.0
44	144180.0
45	144180.0
46	183934.0
47	223688.0
48	223688.0
49	223688.0
50	179341.0
51	134994.0
52	134994.0
53	134994.0
54	102107.5
55	69221.0
56	69221.0
57	69221.0
58	54518.0
59	39815.0
60	39815.0
61	39815.0
62	34426.0
63	29037.0
64	29037.0
65	29037.0
66	23514.0
67	17991.0
68	17991.0
69	17991.0
70	13082.0
71	8173.0
72	8173.0
73	8173.0
74	6123.0
75	4073.0
76	4073.0
77	4073.0
78	2691.5
79	1310.0
80	1310.0
81	1310.0
82	879.0
83	448.0
84	448.0
85	448.0
86	295.0
87	142.0
88	142.0
89	142.0
90	104.0
91	66.0
92	66.0
93	66.0
94	52.0
95	38.0
96	38.0
97	38.0
98	311.5
99	585.0
100	585.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007816313565978499
2	0.003601634682362642
3	0.004674462034555769
4	0.0157858881822703
5	0.03578645524815646
6	0.047970708748064125
7	0.06590225163472067
8	0.08789521235467979
9	0.09555826487034498
10	0.11916046661859378
11	0.11939035819406374
12	0.13249417799585123
13	0.1216892739487633
14	0.12437134232924613
15	0.11402622143309811
16	0.11218708882933846
17	0.12191916552423325
18	0.12881591278833193
19	0.1290458043638019
20	0.11885394451796717
21	0.12659362755878903
22	0.14690071672530178
23	0.13693874845493703
24	0.13119145906818813
25	0.12912243488895853
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1304963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.61115345486387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.18410266066557	15.41721208442501
2	14.663107241345314	6.630995359183692
3	5.868137379455851	3.9805606434329706
4	2.981774281127112	2.6968542335332613
5	1.7480443005234794	1.9762648962518286
6	1.1079662035447702	1.5031436310692246
7	0.7773878419460191	1.2304345051730832
8	0.5558976419293393	1.0055589509489007
9	0.42034762965798983	0.8554090282726587
>10	2.8680232912435843	12.763896638101336
>50	0.355747345745856	5.729943552175406
>100	0.3737382973821315	17.68926815073993
>500	0.05804654754980375	9.18089488506952
>1k	0.03564261691654616	15.298139868475772
>5k	0.001357813977773187	1.8724223813372256
>10k+	6.789069888865936E-4	2.169001191810152
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	14967	1.1469290700196098	No Hit
TCCATGTACTCTGCGTTGATACCAC	13292	1.018572940382218	No Hit
GAGTACATGGAAGCAGTGGTATCAA	6493	0.4975619998421411	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6347	0.48637394316926996	No Hit
CATGTACTCTGCGTTGATACCACTG	6005	0.46016630356569493	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5550	0.4252994146194183	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4984	0.38192653738075333	No Hit
GTCCTACAGTGGACATTTCTAAATT	4512	0.3457569295068136	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4456	0.3414656200980411	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4340	0.33257647917986943	No Hit
CCCATGTACTCTGCGTTGATACCAC	4320	0.3310438686767364	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4142	0.3174036351988524	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4026	0.3085144942806808	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3985	0.305372642749258	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3860	0.2957938271046765	No Hit
GCGTTGATACCACTGCTTCCATGTA	3654	0.2800079389224062	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3417	0.2618465044602797	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	3364	0.2577850866269772	No Hit
TTGTAGAACAGTGTATATCAATGAG	3354	0.2570187813754106	No Hit
GATATACACTGTTCTACAAATCCCG	3228	0.24736333520567252	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	3210	0.24598398575285274	No Hit
GTATCAACGCAGAGTACATGGAAGC	3129	0.23977691321516395	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3088	0.23663506168374124	No Hit
GTGTATATCAATGAGTTACAATGAA	2847	0.2181671051209881	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2831	0.21694101671848168	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2736	0.20966111682859975	No Hit
GTACATGGGGTGGTATCAACGCAAA	2703	0.2071323094984302	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2495	0.19119316026584662	No Hit
CTCTAATACTTGTAATGCTAGAGGT	2460	0.1885110918853638	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2332	0.17870238466531235	No Hit
CAGTGGTATCAACGCAGAGTACATG	2317	0.17755292678796256	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2184	0.16736106694212788	No Hit
GTATCAACGCAGAGTACATGGGAAG	2166	0.16598171748930812	No Hit
CCATTGGGATGTCCTGATCCAACAT	2161	0.16559856486352487	No Hit
GATATACACTGTTCTACAATGCCGG	2125	0.16283986595788538	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2098	0.1607708417786558	No Hit
CTTCTACACCATTGGGATGTCCTGA	2098	0.1607708417786558	No Hit
ATCTATAACTTTATAGATGCAACAC	2003	0.15349094188877385	No Hit
GTGTATATCAATGAGTTACAATGAG	1963	0.15042572088250777	No Hit
GTCAGGATACCGCGGCCGTTAAACT	1958	0.15004256825672452	No Hit
GATATATTTTGATCAACGGACCAAG	1951	0.14950615458062796	No Hit
TATCAACGCAGAGTACATGGAAGCA	1892	0.1449849535963855	No Hit
CTGTTAGTATGAGTAACAAGAATTC	1888	0.14467843149575887	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1848	0.1416132104894928	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1804	0.13824146738260013	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	1792	0.1373219010807203	No Hit
GTTATATAATTTAAGCTCCATAGGG	1723	0.1320343948449113	No Hit
CCTCTAGCATTACAAGTATTAGAGG	1705	0.13065504539209158	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	1676	0.12843276016254868	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1659	0.12713004123488558	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	1652	0.12659362755878903	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	1584	0.1213827518481367	No Hit
GTTATAGATTAACCCAATTTTAAGT	1582	0.12122949079782339	No Hit
ATCGTAAATAGATAGAAACCGACCT	1567	0.1200800329204736	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1505	0.11532894036076118	No Hit
GTACATGGGTGGTATCAACGCAAAA	1501	0.11502241826013458	No Hit
GTACATGGGAGAAATCGTAAATAGA	1473	0.11287676355574833	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1470	0.11264687198027837	No Hit
ACCTATAACTTCTCTGTTAACCCAA	1451	0.11119089200230198	No Hit
GTATCAACGCAGAGTACATGGGAGA	1448	0.11096100042683203	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	1443	0.11057784780104878	No Hit
ATAAATAATCCACCTATAACTTCTC	1412	0.10820230152119256	No Hit
CTATAGAACTAGTACCGCAAGGGAA	1412	0.10820230152119256	No Hit
ATACCACTGCTTCCATGTACTCTGC	1387	0.10628653839227625	No Hit
CACTATAAATAATCCACCTATAACT	1383	0.10598001629164966	No Hit
GACTATAGGCAATAATCACACTATA	1377	0.10552023314070974	No Hit
GTATCAACGCAGAGTACATGGGCAG	1365	0.1046006668388299	No Hit
GCGTTGATACCACTGCTTCCCATGT	1356	0.10391099211242004	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1353	0.1036811005369501	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	1340	0.10268490370991361	No Hit
CTGTAGAACATATTAGATGAGTGAG	1323	0.10138218478225054	No Hit
CCTATAACTTCTCTGTTAACCCAAC	1316	0.10084577110615396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.663052515665195E-5	0.0
2	0.0	0.0	0.0	2.2989157546995587E-4	0.0
3	0.0	0.0	0.0	2.2989157546995587E-4	0.0
4	0.0	0.0	0.0	2.2989157546995587E-4	0.0
5	0.0	0.0	0.0	2.2989157546995587E-4	0.0
6	0.0	0.0	0.0	2.2989157546995587E-4	0.0
7	0.0	0.0	0.0	3.8315262578325973E-4	0.0
8	0.0	0.0	0.0	3.8315262578325973E-4	0.0
9	0.0	0.0	0.0	6.130442012532156E-4	0.0
10	0.0	0.0	0.0	6.130442012532156E-4	0.0
11	0.0	0.0	0.0	6.130442012532156E-4	0.0
12	0.0	0.0	0.0	6.130442012532156E-4	0.0
13	0.0	0.0	0.0	6.130442012532156E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATGG	45	3.5348705E-5	16.890732	13
CTAATGT	35	0.0021717043	16.28374	4
CTTGCTA	110	0.0	15.547747	9
AACTTGC	95	3.0377123E-10	15.000484	7
TAACTTT	325	0.0	14.9081745	6
AATACCG	45	6.761602E-4	14.777688	5
TGCGGCG	40	0.0052746795	14.2515545	18
TCGAGTT	40	0.0052760225	14.251008	12
TTAACCG	40	0.005282743	14.248273	4
TATTAAC	40	0.005294858	14.243354	2
GGTGTGC	55	1.9579566E-4	13.81969	8
TGCCGTC	55	1.9592319E-4	13.818628	7
ACTTTAT	350	0.0	13.301451	8
GACGGTT	50	0.0014980886	13.30145	18
TAAGACC	50	0.0015006902	13.2983885	4
CGGTTGT	230	0.0	13.219339	10
TAGGACC	805	0.0	12.9797945	4
TTGCGGT	90	5.393067E-7	12.668534	17
CCGGTGT	195	0.0	12.668048	13
TAGAAAT	510	0.0	12.6651325	4
>>END_MODULE