##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062664_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1304963
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14102622066679	32.0	32.0	32.0	32.0	32.0
2	31.473330661482358	32.0	32.0	32.0	32.0	32.0
3	31.562055782424483	32.0	32.0	32.0	32.0	32.0
4	31.65613737707506	32.0	32.0	32.0	32.0	32.0
5	31.617058874466174	32.0	32.0	32.0	32.0	32.0
6	35.202224890667395	36.0	36.0	36.0	36.0	36.0
7	35.22653362585759	36.0	36.0	36.0	36.0	36.0
8	35.17866023787648	36.0	36.0	36.0	36.0	36.0
9	35.281540549425536	36.0	36.0	36.0	36.0	36.0
10	35.20449085529628	36.0	36.0	36.0	36.0	36.0
11	35.29322057406991	36.0	36.0	36.0	36.0	36.0
12	35.21811116483762	36.0	36.0	36.0	36.0	36.0
13	35.244065923708185	36.0	36.0	36.0	36.0	36.0
14	35.21709887560031	36.0	36.0	36.0	36.0	36.0
15	35.20121490034583	36.0	36.0	36.0	36.0	36.0
16	35.207605886143895	36.0	36.0	36.0	36.0	36.0
17	35.201436362563534	36.0	36.0	36.0	36.0	36.0
18	35.20873158855845	36.0	36.0	36.0	36.0	36.0
19	35.151826526882374	36.0	36.0	36.0	36.0	36.0
20	35.11183841993988	36.0	36.0	36.0	36.0	36.0
21	35.07357066828715	36.0	36.0	36.0	36.0	36.0
22	35.045500906922264	36.0	36.0	36.0	36.0	36.0
23	35.0105658168086	36.0	36.0	36.0	36.0	36.0
24	34.98753144725176	36.0	36.0	36.0	36.0	36.0
25	34.97879096955239	36.0	36.0	36.0	36.0	36.0
26	34.93216665913133	36.0	36.0	36.0	36.0	36.0
27	34.88422890150908	36.0	36.0	36.0	36.0	36.0
28	34.83627198625555	36.0	36.0	36.0	32.0	36.0
29	34.79340333787241	36.0	36.0	36.0	32.0	36.0
30	34.748776785242185	36.0	36.0	36.0	32.0	36.0
31	34.71944645173848	36.0	36.0	36.0	32.0	36.0
32	34.666060263777595	36.0	36.0	36.0	32.0	36.0
33	34.63943498781192	36.0	36.0	36.0	32.0	36.0
34	34.59447892392351	36.0	36.0	36.0	32.0	36.0
35	34.4980562667294	36.0	36.0	36.0	32.0	36.0
36	34.41759881314643	36.0	36.0	36.0	32.0	36.0
37	34.32881468669993	36.0	36.0	36.0	32.0	36.0
38	34.240843610125346	36.0	36.0	36.0	32.0	36.0
39	34.07193767179606	36.0	36.0	36.0	32.0	36.0
40	34.003726542438365	36.0	36.0	36.0	32.0	36.0
41	33.99611176715355	36.0	36.0	36.0	32.0	36.0
42	33.82572685968874	36.0	36.0	36.0	32.0	36.0
43	33.86004354146439	36.0	36.0	36.0	32.0	36.0
44	33.81146975048335	36.0	36.0	36.0	32.0	36.0
45	33.622337185038965	36.0	36.0	36.0	21.0	36.0
46	33.59971355509696	36.0	36.0	36.0	21.0	36.0
47	33.50594844451528	36.0	36.0	36.0	21.0	36.0
48	33.46685384949612	36.0	36.0	36.0	21.0	36.0
49	33.47316360693751	36.0	36.0	36.0	21.0	36.0
50	32.9405891201513	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	5.0
21	33.0
22	99.0
23	361.0
24	1023.0
25	2650.0
26	5716.0
27	11170.0
28	19313.0
29	28966.0
30	39356.0
31	53633.0
32	75842.0
33	103851.0
34	202268.0
35	760675.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97616213610315	21.231426248015875	13.212608576020651	23.579803039860323
2	11.877133315043215	22.7318847354651	43.50184765965665	21.889134289835035
3	18.407622031121402	28.19103202105208	31.125618221025412	22.275727726801097
4	9.82884467280481	18.719496074577286	40.741941292994774	30.709717959623127
5	9.700504918530257	41.38975587813601	36.97246588600596	11.93727331732777
6	27.69155081328537	40.60993720851463	19.588353305940288	12.110158672259711
7	24.876797273179392	34.498066228697674	23.063795678498163	17.56134081962477
8	24.204670937030397	38.43994044275585	21.70682233902417	15.648566281189582
9	25.85299353314998	15.700061994094852	21.626436918134846	36.82050755462033
10	14.603249287527692	29.06105383830806	35.596794698393744	20.7389021757705
11	32.70031410852262	23.223953476075568	26.53554162072028	17.540190794681536
12	22.754514878965917	26.964212778446594	33.429836708013944	16.851435634573548
13	28.26133767777324	23.067244052130214	28.3945215304955	20.276896739601046
14	20.166318891799996	24.798480876469295	27.950141115112075	27.08505911661863
15	22.943409123477064	32.57096178205819	26.407338752133203	18.078290342331545
16	21.40221600152648	28.578051638245682	31.191842220813925	18.827890139413913
17	18.806893375520993	29.35309276968006	31.48625669846578	20.35375715633317
18	19.43204519974896	27.558942284187367	35.210193698978436	17.798818817085234
19	22.152352212284946	29.086035389509128	28.54395105455097	20.217661343654957
20	20.97814267530957	29.244047532382144	32.027344836596896	17.750464955711386
21	21.605593415292233	27.436793227087662	27.66553534468027	23.29207801293983
22	20.42693930785777	31.13452258799675	28.68502785136437	19.753510252781115
23	19.81971902651646	30.161238287982112	29.32995035108275	20.689092334418678
24	20.982357354193184	30.960111512740205	29.097376707232314	18.960154425834297
25	21.807744740655483	28.959978175626432	29.609498506854216	19.622778576863865
26	17.317272597000834	29.654863777746954	32.77364952109753	20.254214104154677
27	20.003862178467894	31.41552672374619	30.477645726353924	18.102965371431985
28	18.669418213389957	29.443823311465533	30.02414627847686	21.862612196667644
29	19.42261964515469	28.98940429728659	30.12805727058928	21.45991878696944
30	20.927413267655865	28.318274157964634	30.85022333966557	19.904089234713933
31	20.338507681826997	27.6094418002656	30.439790246926542	21.61226027098086
32	17.831999834478065	30.829303206297805	30.93612615836618	20.402570800857955
33	18.9728750930103	30.064300673658945	31.127625840732648	19.835198392598105
34	18.019208207435767	30.687613365283156	33.318032771810394	17.97514565547069
35	19.971600727376945	31.032220836912614	30.29082050602201	18.70535792968843
36	18.7920270536406	29.970045127716265	31.19644005232332	20.04148776631981
37	20.309234821217153	29.62957570444526	28.872006332746597	21.189183141590988
38	20.054514955596442	31.275829276385615	28.35658942054296	20.313066347474987
39	19.62423455684184	29.155309384250742	29.951730432203828	21.268725626703592
40	22.33534590635903	29.56306040860929	29.51777176824171	18.58382191678998
41	20.445330633895367	27.84722631982669	32.15156291787583	19.55588012840211
42	20.986495402551643	31.811629908280924	29.34826504659519	17.85360964257224
43	18.370635795804173	30.94900008659249	29.572256071628082	21.10810804597525
44	18.490639198199492	30.352508078773116	31.012373530897047	20.14447919213035
45	19.45763979515128	30.53289633499187	30.220167161827575	19.78929670802927
46	20.589657468690636	28.347054049891145	29.58854709067934	21.47474139073888
47	22.357185606028676	28.742040962080917	29.397538474270917	19.50323495761949
48	19.03923712779596	31.42794086882157	27.509438964936173	22.0233830384463
49	19.442926734321205	28.64357073725462	31.691473244835294	20.22202928358888
50	17.787874282929312	32.494739310416705	27.497658935662493	22.219727470991494
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	37.5
2	48.0
3	223.5
4	399.0
5	438.0
6	477.0
7	455.5
8	434.0
9	579.0
10	724.0
11	1109.0
12	1494.0
13	2749.0
14	4004.0
15	5624.0
16	7244.0
17	9395.5
18	11547.0
19	12361.0
20	13175.0
21	15332.5
22	17490.0
23	17178.5
24	16867.0
25	20958.0
26	25049.0
27	35875.5
28	46702.0
29	52082.5
30	57463.0
31	58672.0
32	59881.0
33	71730.0
34	83579.0
35	97116.5
36	110654.0
37	118157.0
38	125660.0
39	116633.0
40	107606.0
41	103884.0
42	100162.0
43	88135.5
44	76109.0
45	80810.5
46	85512.0
47	88656.0
48	91800.0
49	78413.0
50	65026.0
51	54504.0
52	43982.0
53	37480.0
54	30978.0
55	27734.0
56	24490.0
57	24864.5
58	25239.0
59	22023.5
60	18808.0
61	17620.0
62	16432.0
63	14377.5
64	12323.0
65	10843.5
66	9364.0
67	7898.5
68	6433.0
69	5018.0
70	3603.0
71	2665.5
72	1728.0
73	1420.0
74	1112.0
75	888.5
76	665.0
77	504.0
78	343.0
79	261.0
80	179.0
81	132.0
82	85.0
83	61.0
84	37.0
85	22.0
86	7.0
87	5.5
88	4.0
89	5.0
90	6.0
91	4.0
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	2.5
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01777828183634325
2	0.005287506235808984
3	6.896747264098676E-4
4	6.130442012532156E-4
5	0.0
6	6.896747264098676E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.532610503133039E-4
47	0.0
48	0.0
49	0.0
50	7.663052515665195E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1304963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.3595470158946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.81292244415462	23.23833640180414
2	13.607892060327515	8.80690445826774
3	5.24909011191993	5.095745348020212
4	2.6627676796203974	3.4466382368432322
5	1.4744430269238968	2.3856154226000887
6	0.9572325233245381	1.8585366506198284
7	0.6472620643082431	1.4661575041111308
8	0.48863907308256666	1.2649711247374775
9	0.36444591139537064	1.0613974144094147
>10	2.2164177537704464	13.645674662223614
>50	0.2388871294996736	5.493691253675762
>100	0.2322242925852945	15.868112528640141
>500	0.03018678106524887	6.6665224587116905
>1k	0.017113765643290698	8.575803168882103
>5k	4.753823789802972E-4	1.1258933664534714
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	8070	0.6184083380141812	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	6568	0.5033092892288901	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	3549	0.2719617337809578	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	3120	0.23908723848875407	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	3031	0.23226712174981207	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2982	0.2285122260171361	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2348	0.17992847306781878	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	2248	0.17226542055215358	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2246	0.17211215950184028	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	2131	0.1632996491088253	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	2002	0.15341431136361722	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	1972	0.15111539560891765	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	1952	0.1495827851057846	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1937	0.14843332722843483	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1916	0.14682408620014514	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	1914	0.14667082514983185	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	1860	0.1425327767913726	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	1855	0.14214962416558935	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1834	0.1405403831372997	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1805	0.13831809790775676	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	1787	0.13693874845493703	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1787	0.13693874845493703	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1739	0.13326048324741774	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	1725	0.1321876558952246	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	1693	0.12973547909021177	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1686	0.1291990654141152	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1679	0.12866265173801864	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	1668	0.12781971596129546	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1635	0.12529090863112594	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	1610	0.12337514550220963	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT	1599	0.12253220972548648	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	1511	0.1157887235117011	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	1492	0.11433274353372472	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	1491	0.11425611300856806	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	1467	0.11241698040480841	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	1439	0.11027132570042217	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	1437	0.11011806465010886	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	1436	0.1100414341249522	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT	1420	0.10881534572244576	No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT	1397	0.10705284364384278	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	1375	0.10536697209039643	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	1374	0.1052903415652398	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC	1329	0.10184196793319043	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	1317	0.10092240163131062	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.663052515665195E-5	0.0
8	7.663052515665195E-5	0.0	0.0	1.532610503133039E-4	0.0
9	7.663052515665195E-5	0.0	0.0	1.532610503133039E-4	0.0
10	7.663052515665195E-5	0.0	0.0	1.532610503133039E-4	0.0
11	7.663052515665195E-5	0.0	0.0	1.532610503133039E-4	0.0
12	7.663052515665195E-5	0.0	0.0	1.532610503133039E-4	0.0
13	7.663052515665195E-5	0.0	0.0	1.532610503133039E-4	0.0
14	7.663052515665195E-5	0.0	0.0	1.532610503133039E-4	0.0
15	7.663052515665195E-5	0.0	0.0	1.532610503133039E-4	0.0
16	7.663052515665195E-5	0.0	0.0	3.8315262578325973E-4	0.0
17	7.663052515665195E-5	0.0	0.0	6.896747264098675E-4	0.0
18	7.663052515665195E-5	0.0	0.0	8.429357767231714E-4	0.0
19	7.663052515665195E-5	0.0	0.0	0.001379349452819735	0.0
20	7.663052515665195E-5	0.0	0.0	0.0020690241792296026	0.0
21	7.663052515665195E-5	0.0	0.0	0.0033717431068926856	0.0
22	7.663052515665195E-5	0.0	0.0	0.00513424518549568	0.0
23	7.663052515665195E-5	0.0	0.0	0.00942555459426819	0.0
24	7.663052515665195E-5	0.0	0.0	0.015172843981017087	0.0
25	7.663052515665195E-5	0.0	0.0	0.01885110918853638	0.0
26	7.663052515665195E-5	0.0	0.0	0.02436850699981532	0.0
27	7.663052515665195E-5	0.0	0.0	0.029272860609841047	0.0
28	7.663052515665195E-5	0.0	0.0	0.040307656232398924	0.0
29	7.663052515665195E-5	0.0	0.0	0.05433104233606623	0.0
30	1.532610503133039E-4	0.0	0.0	0.08620934080123345	0.0
31	1.532610503133039E-4	0.0	0.0	0.14207299364043272	0.0
32	1.532610503133039E-4	0.0	0.0	0.21249644625939587	0.0
33	1.532610503133039E-4	0.0	0.0	0.2770193484412968	0.0
34	1.532610503133039E-4	0.0	0.0	0.35993357666079423	0.0
35	1.532610503133039E-4	0.0	0.0	0.45296303420096967	0.0
36	1.532610503133039E-4	0.0	0.0	0.5943463531149925	0.0
37	1.532610503133039E-4	0.0	0.0	0.7974938753052768	0.0
38	1.532610503133039E-4	0.0	0.0	1.016733807778458	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGTTA	30	0.0057453257	29.33318	28
CCGGTAT	55	4.9616483E-6	27.999853	28
TAGGACC	1040	0.0	26.442171	4
TCGTATA	120	2.3646862E-11	23.833208	44
CTCTAGG	225	0.0	23.471039	1
AGGACCT	1720	0.0	22.383604	5
CTGTAGG	2750	0.0	22.244144	1
GGCGTCT	50	0.0025806248	21.999886	39
CGCGTCT	80	3.6160727E-6	21.999886	23
TGTAGGA	2900	0.0	21.849	2
ATACATG	255	0.0	21.572649	1
GGACCTG	1775	0.0	21.566084	6
TCCTACA	2880	0.0	21.46599	2
CCTACAG	2770	0.0	21.443932	3
GTCCTAT	165	0.0	21.337309	1
GATATAC	1480	0.0	20.963367	1
GTCCTAC	3000	0.0	20.83055	1
GCCGGTA	85	6.114322E-6	20.705774	27
ATATACA	1570	0.0	20.319143	2
ACTGTTC	1800	0.0	20.166561	8
>>END_MODULE