FastQCFastQC Report
Thu 2 Feb 2017
SRR4062663_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062663_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879233
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12730.14478528444678487No Hit
GCGCAAGACGGACCAGAGCGAAAGC10000.11373549445937538No Hit
TATCAACGCAGAGTACTTTTTTTTT9130.10384050644140974No Hit
GATTAAGAGGGACGGCCGGGGGCAT8990.10224820951897848No Hit
GTATCTGATCGTCTTCGAACCTCCG8850.10065591259654721No Hit
GAACTACGACGGTATCTGATCGTCT8820.10031470611316909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAT400.005307721414.2374091
ATACTAA707.3075807E-613.5617532
CGAACGA655.4313015E-513.157948516
CATACTA655.492589E-513.1422241
CGTCTTA957.3587216E-813.00183215
TCTCGTC1102.733941E-912.9563712
CGGACAG1102.744855E-912.9526825
CGACCCG1207.403287E-1012.66484455
GTATTTA751.4903346E-512.6554741
TTAGCTC701.09248394E-412.2111384
CGAGATT701.09248394E-412.2111384
TAGTACG550.00308304512.0822882
TAGAAAT951.0412878E-611.9969084
AACCGCG802.858907E-511.8766737
TACTAAC802.8772967E-511.8692353
ATAGGGT1459.822543E-1111.7873793
TATACTG1052.7195347E-711.7602125
TCTATAG907.490489E-611.6054753
CCGACCC1251.8337232E-811.3970624
TAATATA1354.7311914E-911.2563574
Produced by FastQC (version 0.11.5)