##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062663_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	879233
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.136854508418132	32.0	32.0	32.0	32.0	32.0
2	30.83031346639628	32.0	32.0	32.0	32.0	32.0
3	30.8429210459571	32.0	32.0	32.0	32.0	32.0
4	30.790379796936648	32.0	32.0	32.0	32.0	32.0
5	30.763696312581533	32.0	32.0	32.0	32.0	32.0
6	34.19430913079923	36.0	36.0	36.0	32.0	36.0
7	34.1295822609024	36.0	36.0	36.0	32.0	36.0
8	34.06879860059848	36.0	36.0	36.0	32.0	36.0
9	34.282988695829204	36.0	36.0	36.0	32.0	36.0
10	33.94572997146376	36.0	36.0	36.0	32.0	36.0
11	34.2477102201578	36.0	36.0	36.0	32.0	36.0
12	34.0408333172208	36.0	36.0	36.0	32.0	36.0
13	34.16765976709245	36.0	36.0	36.0	32.0	36.0
14	34.06242941290875	36.0	36.0	36.0	32.0	36.0
15	34.04287031992657	36.0	36.0	36.0	32.0	36.0
16	34.057130476221886	36.0	36.0	36.0	32.0	36.0
17	33.938301906320625	36.0	36.0	36.0	32.0	36.0
18	33.99527997697994	36.0	36.0	36.0	32.0	36.0
19	33.98482541032923	36.0	36.0	36.0	32.0	36.0
20	33.90763313023965	36.0	36.0	36.0	32.0	36.0
21	33.86919849459699	36.0	36.0	36.0	32.0	36.0
22	33.88238271311472	36.0	36.0	36.0	32.0	36.0
23	33.81340327308006	36.0	36.0	36.0	32.0	36.0
24	33.78899336125919	36.0	36.0	36.0	32.0	36.0
25	33.19204693181443	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	39.0
5	81.0
6	132.0
7	36.0
8	141.0
9	158.0
10	82.0
11	46.0
12	61.0
13	41.0
14	174.0
15	340.0
16	461.0
17	732.0
18	866.0
19	1070.0
20	1405.0
21	1759.0
22	2434.0
23	3385.0
24	4677.0
25	6535.0
26	8929.0
27	12242.0
28	17011.0
29	23316.0
30	32087.0
31	47270.0
32	72287.0
33	109882.0
34	236196.0
35	295352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.41861179326246	18.08458727221232	11.12785683655932	26.368944097965908
2	17.067256290917694	20.325771948262293	36.62904172410499	25.97793003671503
3	18.93246343480254	23.936996052016475	28.753058237022326	28.37748227615866
4	12.851619420073233	15.395098002413802	36.17165522897564	35.58162734853733
5	14.707297842492755	36.30751446928143	33.75737117020562	15.227816518020184
6	33.67866863285908	35.52607025496782	16.922567519593652	13.872693592579443
7	29.628769720683874	30.56862490695912	21.28684362075284	18.515761751604174
8	27.688553010667132	32.79718791157129	20.02429159936391	19.489967478397666
9	27.415693497965627	14.24951218234931	18.943718252007624	39.391076067677446
10	15.95857577172267	26.399576420755384	31.849060041219268	25.792787766302677
11	37.17858936300315	21.26738928192813	22.560034798491003	18.993986556577724
12	24.333373572734995	24.017570193080797	28.74835720744626	22.900699026737943
13	29.20822577026921	19.586137944149577	25.34722894392524	25.85840734165597
14	23.12165847714811	20.13255063172219	25.09932756745677	31.64646332367293
15	25.09905995518391	27.33041391120584	22.242261937293907	25.328264196316336
16	25.601127676580898	25.751082246153217	24.003775621508748	24.64401445575714
17	23.931233644817244	25.872333350794047	25.326875906723362	24.86955709766535
18	24.680937321706967	24.774432622457777	26.53011721645927	24.014512839375985
19	25.40126317899287	25.126238181896866	25.46275945163546	24.009739187474803
20	25.358552013527902	24.61428969977852	25.17260030631359	24.854557980379987
21	26.42583992511223	24.360390564323083	24.364262498946605	24.849507011618083
22	25.646478326189794	24.849962811051668	24.73913632796438	24.76442253479416
23	24.318485178419305	24.524397410608124	25.526966757930126	25.630150653042445
24	24.572901920902154	25.277851486828606	25.1850350254021	24.964211566867142
25	24.50455416138062	24.870230885390924	25.428027395575437	25.197187557653017
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	81.0
2	227.5
3	374.0
4	374.0
5	374.0
6	932.5
7	1491.0
8	1491.0
9	1491.0
10	1749.0
11	2007.0
12	2007.0
13	2007.0
14	2488.0
15	2969.0
16	2969.0
17	2969.0
18	4760.5
19	6552.0
20	6552.0
21	6552.0
22	10476.5
23	14401.0
24	14401.0
25	14401.0
26	21189.5
27	27978.0
28	27978.0
29	27978.0
30	34293.5
31	40609.0
32	40609.0
33	40609.0
34	49726.5
35	58844.0
36	58844.0
37	58844.0
38	65862.5
39	72881.0
40	72881.0
41	72881.0
42	82230.5
43	91580.0
44	91580.0
45	91580.0
46	102249.0
47	112918.0
48	112918.0
49	112918.0
50	113386.0
51	113854.0
52	113854.0
53	113854.0
54	105560.5
55	97267.0
56	97267.0
57	97267.0
58	88862.0
59	80457.0
60	80457.0
61	80457.0
62	71576.0
63	62695.0
64	62695.0
65	62695.0
66	52349.5
67	42004.0
68	42004.0
69	42004.0
70	32363.0
71	22722.0
72	22722.0
73	22722.0
74	17886.5
75	13051.0
76	13051.0
77	13051.0
78	10761.0
79	8471.0
80	8471.0
81	8471.0
82	5996.5
83	3522.0
84	3522.0
85	3522.0
86	2523.5
87	1525.0
88	1525.0
89	1525.0
90	1026.5
91	528.0
92	528.0
93	528.0
94	305.5
95	83.0
96	83.0
97	83.0
98	226.0
99	369.0
100	369.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007961484612156278
2	0.0032983293393218866
3	0.005686774722968769
4	0.013875730324043798
5	0.03434811932673137
6	0.04287828141118452
7	0.06676273524765335
8	0.08416426589993778
9	0.09451419589574095
10	0.11407670094275352
11	0.11760250127099414
12	0.13136449610057857
13	0.12124203709369416
14	0.12260686302720666
15	0.11111957808680975
16	0.10998222314221599
17	0.12181071456599103
18	0.12670134082774417
19	0.12863484423355356
20	0.1191947981934254
21	0.12727001830004106
22	0.14603637488583798
23	0.13523150291219732
24	0.13147823159503794
25	0.12908978621139106
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	879233.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.11873991807292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.12250110432183	51.17457505066598
2	9.443113267704836	11.354161411161016
3	2.2863809091533285	4.123630176931082
4	0.9317432496019314	2.240609203729545
5	0.49966083283757784	1.5019489828305024
6	0.3232012922026724	1.1658272656270532
7	0.20184915320895203	0.8494441721116568
8	0.15682672275094095	0.7542579965814038
9	0.12054717230217844	0.652242968954441
>10	0.7151025892473883	8.382136422180363
>50	0.09868476453997851	4.153956408099534
>100	0.09393672280009463	10.955074741022898
>500	0.006262448171517709	2.5469011402777566
>1k	1.8977115671265786E-4	0.1452340598268045
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1273	0.14478528444678487	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1000	0.11373549445937538	No Hit
TATCAACGCAGAGTACTTTTTTTTT	913	0.10384050644140974	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	899	0.10224820951897848	No Hit
GTATCTGATCGTCTTCGAACCTCCG	885	0.10065591259654721	No Hit
GAACTACGACGGTATCTGATCGTCT	882	0.10031470611316909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.1373549445937539E-4	0.0
5	0.0	0.0	0.0	2.2747098891875078E-4	0.0
6	0.0	0.0	0.0	2.2747098891875078E-4	0.0
7	0.0	0.0	0.0	2.2747098891875078E-4	0.0
8	0.0	0.0	0.0	2.2747098891875078E-4	0.0
9	0.0	0.0	0.0	2.2747098891875078E-4	0.0
10	0.0	0.0	0.0	2.2747098891875078E-4	0.0
11	0.0	0.0	0.0	3.4120648337812614E-4	1.1373549445937539E-4
12	0.0	0.0	0.0	3.4120648337812614E-4	3.4120648337812614E-4
13	0.0	0.0	0.0	3.4120648337812614E-4	4.5494197783750156E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAAT	40	0.0053077214	14.237409	1
ATACTAA	70	7.3075807E-6	13.561753	2
CGAACGA	65	5.4313015E-5	13.1579485	16
CATACTA	65	5.492589E-5	13.142224	1
CGTCTTA	95	7.3587216E-8	13.001832	15
TCTCGTC	110	2.733941E-9	12.95637	12
CGGACAG	110	2.744855E-9	12.952682	5
CGACCCG	120	7.403287E-10	12.6648445	5
GTATTTA	75	1.4903346E-5	12.655474	1
TTAGCTC	70	1.09248394E-4	12.211138	4
CGAGATT	70	1.09248394E-4	12.211138	4
TAGTACG	55	0.003083045	12.082288	2
TAGAAAT	95	1.0412878E-6	11.996908	4
AACCGCG	80	2.858907E-5	11.876673	7
TACTAAC	80	2.8772967E-5	11.869235	3
ATAGGGT	145	9.822543E-11	11.787379	3
TATACTG	105	2.7195347E-7	11.760212	5
TCTATAG	90	7.490489E-6	11.605475	3
CCGACCC	125	1.8337232E-8	11.397062	4
TAATATA	135	4.7311914E-9	11.256357	4
>>END_MODULE