FastQCFastQC Report
Thu 2 Feb 2017
SRR4062663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879233
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9670.10998222314221599No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC9300.1057740098472191No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG8920.10145206105776285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13350.020.1093141
ACTAGAC550.004482823419.9998723
TAGGACG2250.019.5554314
GTAAACG2003.6379788E-1217.59988627
CGCGTAA1408.3649866E-817.28560310
GCGTAAC1408.3649866E-817.28560311
GTATAGA1053.765314E-516.765611
CGTGCGT800.001987855416.4998939
CGTCTTA1501.8360333E-716.13322815
GTATTAG3150.016.0670451
CGCTTCG2353.6379788E-1215.9147932
TAACGGC1958.894858E-1015.7947736
GAGCGAA4050.015.75298416
AACCGCG2700.015.481387
TACCGGC1004.939893E-415.4007762
GCGCCGA3000.015.399919
GTATAGC1158.19799E-515.3077321
GGCGTTA2303.6379788E-1115.30424942
TAAACGC2303.6379788E-1115.30424928
AACGCAG17450.015.1288426
Produced by FastQC (version 0.11.5)