##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062663_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	879233
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.160245350208648	32.0	32.0	32.0	32.0	32.0
2	31.140897805246162	32.0	32.0	32.0	32.0	32.0
3	31.311852489613106	32.0	32.0	32.0	32.0	32.0
4	31.48053473880075	32.0	32.0	32.0	32.0	32.0
5	31.351070762812586	32.0	32.0	32.0	32.0	32.0
6	34.93694845393656	36.0	36.0	36.0	36.0	36.0
7	34.95048525248711	36.0	36.0	36.0	36.0	36.0
8	34.87975997261249	36.0	36.0	36.0	32.0	36.0
9	35.06813779737567	36.0	36.0	36.0	36.0	36.0
10	34.87379568328304	36.0	36.0	36.0	32.0	36.0
11	35.069267190835646	36.0	36.0	36.0	36.0	36.0
12	34.948372047000056	36.0	36.0	36.0	32.0	36.0
13	35.018148772850886	36.0	36.0	36.0	36.0	36.0
14	34.97653409278314	36.0	36.0	36.0	32.0	36.0
15	34.9057189618679	36.0	36.0	36.0	32.0	36.0
16	34.932769811870116	36.0	36.0	36.0	32.0	36.0
17	34.89343780317618	36.0	36.0	36.0	32.0	36.0
18	34.91527388075743	36.0	36.0	36.0	32.0	36.0
19	34.90245020375714	36.0	36.0	36.0	32.0	36.0
20	34.905646171151446	36.0	36.0	36.0	32.0	36.0
21	34.9002607954888	36.0	36.0	36.0	32.0	36.0
22	34.86404741405293	36.0	36.0	36.0	32.0	36.0
23	34.83526892188988	36.0	36.0	36.0	32.0	36.0
24	34.797476891790915	36.0	36.0	36.0	32.0	36.0
25	34.78033581542094	36.0	36.0	36.0	32.0	36.0
26	34.73210286693061	36.0	36.0	36.0	32.0	36.0
27	34.705179400682184	36.0	36.0	36.0	32.0	36.0
28	34.69763760004459	36.0	36.0	36.0	32.0	36.0
29	34.66949147723072	36.0	36.0	36.0	32.0	36.0
30	34.65543035805071	36.0	36.0	36.0	32.0	36.0
31	34.66052457084754	36.0	36.0	36.0	32.0	36.0
32	34.61114061915328	36.0	36.0	36.0	32.0	36.0
33	34.60026864323791	36.0	36.0	36.0	32.0	36.0
34	34.5809791033776	36.0	36.0	36.0	32.0	36.0
35	34.57449618019342	36.0	36.0	36.0	32.0	36.0
36	34.53308508666076	36.0	36.0	36.0	32.0	36.0
37	34.531447295540545	36.0	36.0	36.0	32.0	36.0
38	34.49823311909358	36.0	36.0	36.0	32.0	36.0
39	34.494157976327095	36.0	36.0	36.0	32.0	36.0
40	34.474282698670315	36.0	36.0	36.0	32.0	36.0
41	34.43476530112041	36.0	36.0	36.0	32.0	36.0
42	34.42461327088496	36.0	36.0	36.0	32.0	36.0
43	34.42009910910987	36.0	36.0	36.0	32.0	36.0
44	34.389471277806905	36.0	36.0	36.0	32.0	36.0
45	34.34892002461236	36.0	36.0	36.0	32.0	36.0
46	34.35143016697508	36.0	36.0	36.0	32.0	36.0
47	34.34312747587955	36.0	36.0	36.0	32.0	36.0
48	34.303260910361644	36.0	36.0	36.0	32.0	36.0
49	34.293065660638305	36.0	36.0	36.0	32.0	36.0
50	33.68476046736189	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	15.0
22	62.0
23	190.0
24	535.0
25	1282.0
26	2769.0
27	5166.0
28	8900.0
29	14576.0
30	22014.0
31	33474.0
32	51473.0
33	88418.0
34	186394.0
35	463961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.04643819161328	18.259728086779266	11.53003512667443	26.163798594933024
2	16.644658047477197	20.614512494867498	36.61233324347902	26.128496214176277
3	18.670313797299908	24.042173265243452	29.128328196262636	28.159184741194
4	12.868829991594946	16.06047543711394	36.31585711637302	34.75483745491809
5	14.848168801671457	36.1669773541257	33.50806896465442	15.476784879548426
6	33.98727525840734	35.77212840296987	16.692067622652495	13.548528715970301
7	29.88824337603727	30.559169820934635	21.043592589896637	18.508994213131462
8	27.881687789243582	32.944737060597134	19.728672604417714	19.44490254574157
9	27.600306175951083	14.197260566880452	18.767380205247072	39.43505305192139
10	16.04728211975665	26.450667797955717	31.627338828274187	25.874711254013445
11	37.536466442911035	21.03515222927256	22.39918201432385	19.029199313492555
12	24.5785815591544	23.805635138808483	28.64087221475991	22.974911087277206
13	29.376399657428692	19.47765836814587	25.275325198212535	25.870616776212902
14	23.381515479969472	20.010964101665884	24.70801255184917	31.899507866515474
15	25.24871109250904	27.232371851375003	21.981886485152398	25.537030570963555
16	25.830013204690903	25.704676689796674	23.77742873618256	24.687881369329858
17	24.244654147421674	25.75142197801948	25.125194345526157	24.878729529032693
18	25.007819315244085	24.774434080613446	26.074544517778563	24.14320208636391
19	25.642008432349563	25.198894945935834	25.09914891729496	24.05994770441965
20	25.708088754630452	24.497488151604866	24.842334170805692	24.952088922958986
21	26.788348481005603	24.345651266501598	24.088154107045572	24.777846145447224
22	25.88335515159235	24.777846145447224	24.576761791243047	24.762036911717374
23	24.53638569070997	24.333367833099988	25.311493085450614	25.81875339073943
24	24.739972225792254	25.255421486682145	24.93673463120697	25.06787165631863
25	24.874180109254315	24.58915896013912	25.215386592632445	25.32127433797412
26	24.52387478631944	25.32275289940209	25.576837994024338	24.576534320254133
27	25.32093313149074	25.09710167839469	24.59120619903939	24.990758991075175
28	24.436070984596803	24.822885401253135	25.626540405103082	25.11450320904698
29	24.603375896946545	25.002814953487867	25.23392547822932	25.15988367133627
30	24.554014692351174	25.128833881348854	25.60004003489405	24.71711139140592
31	25.0298840011692	24.941284050985345	24.56561571278603	25.46321623505942
32	24.739176077331038	25.099717594767256	24.626350466827336	25.53475586107437
33	24.403315162192502	24.693909350536206	25.36233285147396	25.54044263579734
34	25.00599954733273	24.80195807027261	25.58525442061433	24.606787961780324
35	25.684886713760747	24.66729524483271	25.237678749546475	24.410139291860062
36	24.497374416110404	25.050356390171892	25.143164553650738	25.309104640066966
37	25.381326679048673	24.992578758986525	24.657968934287045	24.968125627677757
38	24.62737408627747	24.73667389645293	25.321956750940878	25.31399526632872
39	25.142823347167358	24.720750927228618	24.650462391652724	25.485963333951293
40	25.482778740106433	24.586656779261016	25.4957445864748	24.43481989415775
41	24.359072054847804	25.04671685434919	25.78292670998473	24.811284380818282
42	25.401457861567977	25.256103899648895	25.176147847043957	24.166290391739164
43	24.71858995283389	24.246132708849647	25.60504439665026	25.430232941666205
44	24.328022264860397	24.77670879050263	25.364266354879767	25.531002589757207
45	24.249999715661264	25.120531190253324	25.488579250323863	25.14088984376155
46	24.34053316925093	24.464618593706106	25.540328900302878	25.65451933674009
47	24.66047111516515	24.276955027848135	25.84684605787089	25.21572779911582
48	24.898292034079702	25.207652579009203	25.091301168177267	24.802754218733828
49	24.166176656244705	25.034547156442034	25.481527649667377	25.31774853764588
50	23.935207089823844	25.398529625855293	25.413428992575337	25.252834291745526
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	5.5
2	8.0
3	16.5
4	25.0
5	57.0
6	89.0
7	91.0
8	93.0
9	118.5
10	144.0
11	214.0
12	284.0
13	456.0
14	628.0
15	838.0
16	1048.0
17	1387.5
18	1727.0
19	1974.5
20	2222.0
21	2814.5
22	3407.0
23	3844.5
24	4282.0
25	5424.0
26	6566.0
27	8326.0
28	10086.0
29	11699.5
30	13313.0
31	14333.0
32	15353.0
33	17831.0
34	20309.0
35	23571.0
36	26833.0
37	31406.0
38	35979.0
39	38187.0
40	40395.0
41	43747.0
42	47099.0
43	47519.5
44	47940.0
45	52071.5
46	56203.0
47	59575.0
48	62947.0
49	64155.0
50	65363.0
51	62564.0
52	59765.0
53	58793.5
54	57822.0
55	58354.0
56	58886.0
57	57948.5
58	57011.0
59	52021.5
60	47032.0
61	41530.5
62	36029.0
63	31887.0
64	27745.0
65	23667.0
66	19589.0
67	17584.0
68	15579.0
69	14288.5
70	12998.0
71	10376.0
72	7754.0
73	7025.5
74	6297.0
75	4786.0
76	3275.0
77	2907.0
78	2539.0
79	2199.0
80	1859.0
81	1489.5
82	1120.0
83	925.5
84	731.0
85	607.0
86	483.0
87	360.5
88	238.0
89	159.0
90	80.0
91	56.0
92	32.0
93	22.5
94	13.0
95	9.0
96	5.0
97	3.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014671878785259426
2	0.0036395358227000124
3	3.412064833781262E-4
4	0.0
5	0.0
6	1.1373549445937539E-4
7	1.1373549445937539E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	1.1373549445937539E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	879233.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.01238718104224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.94330240882121	55.01435949410863
2	9.147093434991316	11.710545730836674
3	2.100667163186501	4.034061593651878
4	0.8635706769134104	2.2111688211510385
5	0.46838844717992517	1.4991331316004262
6	0.2916998928905025	1.120344389062524
7	0.18733777899927948	0.839435691005688
8	0.13075249161505983	0.6695823294519352
9	0.09583338004805643	0.552107108565374
>10	0.6103301185902573	7.571584744153154
>50	0.08176670362538549	3.7360829589090594
>100	0.07496364295258724	9.141851643491842
>500	0.004293860186415041	1.8997423640118243
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	967	0.10998222314221599	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	930	0.1057740098472191	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	892	0.10145206105776285	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2747098891875078E-4
2	0.0	0.0	0.0	0.0	2.2747098891875078E-4
3	0.0	0.0	0.0	0.0	2.2747098891875078E-4
4	0.0	0.0	0.0	0.0	2.2747098891875078E-4
5	0.0	0.0	0.0	1.1373549445937539E-4	2.2747098891875078E-4
6	0.0	0.0	0.0	1.1373549445937539E-4	2.2747098891875078E-4
7	0.0	0.0	0.0	2.2747098891875078E-4	2.2747098891875078E-4
8	1.1373549445937539E-4	0.0	0.0	2.2747098891875078E-4	2.2747098891875078E-4
9	1.1373549445937539E-4	0.0	0.0	2.2747098891875078E-4	2.2747098891875078E-4
10	1.1373549445937539E-4	0.0	0.0	3.4120648337812614E-4	3.4120648337812614E-4
11	1.1373549445937539E-4	0.0	0.0	4.5494197783750156E-4	3.4120648337812614E-4
12	1.1373549445937539E-4	0.0	0.0	4.5494197783750156E-4	3.4120648337812614E-4
13	1.1373549445937539E-4	0.0	0.0	4.5494197783750156E-4	3.4120648337812614E-4
14	1.1373549445937539E-4	0.0	0.0	4.5494197783750156E-4	3.4120648337812614E-4
15	1.1373549445937539E-4	1.1373549445937539E-4	0.0	4.5494197783750156E-4	4.5494197783750156E-4
16	1.1373549445937539E-4	1.1373549445937539E-4	0.0	4.5494197783750156E-4	4.5494197783750156E-4
17	1.1373549445937539E-4	1.1373549445937539E-4	0.0	4.5494197783750156E-4	5.686774722968769E-4
18	1.1373549445937539E-4	1.1373549445937539E-4	0.0	4.5494197783750156E-4	6.824129667562523E-4
19	1.1373549445937539E-4	1.1373549445937539E-4	0.0	4.5494197783750156E-4	6.824129667562523E-4
20	1.1373549445937539E-4	1.1373549445937539E-4	0.0	6.824129667562523E-4	0.0010236194501343785
21	1.1373549445937539E-4	1.1373549445937539E-4	0.0	6.824129667562523E-4	0.0010236194501343785
22	1.1373549445937539E-4	1.1373549445937539E-4	0.0	0.0010236194501343785	0.0010236194501343785
23	1.1373549445937539E-4	1.1373549445937539E-4	0.0	0.0012510904390531292	0.0010236194501343785
24	1.1373549445937539E-4	1.1373549445937539E-4	0.0	0.0014785614279718801	0.0010236194501343785
25	1.1373549445937539E-4	1.1373549445937539E-4	0.0	0.0018197679113500062	0.0010236194501343785
26	1.1373549445937539E-4	1.1373549445937539E-4	0.0	0.0021609743947281325	0.0010236194501343785
27	1.1373549445937539E-4	1.1373549445937539E-4	0.0	0.003525800328240637	0.0010236194501343785
28	1.1373549445937539E-4	1.1373549445937539E-4	0.0	0.008530162084453154	0.0010236194501343785
29	1.1373549445937539E-4	1.1373549445937539E-4	0.0	0.020586124497146946	0.0010236194501343785
30	1.1373549445937539E-4	1.1373549445937539E-4	0.0	0.04265081042226577	0.0010236194501343785
31	1.1373549445937539E-4	1.1373549445937539E-4	0.0	0.09110213106195969	0.0010236194501343785
32	1.1373549445937539E-4	1.1373549445937539E-4	0.0	0.1497896462029974	0.0010236194501343785
33	1.1373549445937539E-4	1.1373549445937539E-4	0.0	0.2134815231002476	0.0010236194501343785
34	1.1373549445937539E-4	2.2747098891875078E-4	0.0	0.28763706548776036	0.0010236194501343785
35	1.1373549445937539E-4	2.2747098891875078E-4	0.0	0.36759311809270123	0.0010236194501343785
36	1.1373549445937539E-4	2.2747098891875078E-4	0.0	0.4967966397985517	0.0010236194501343785
37	1.1373549445937539E-4	2.2747098891875078E-4	0.0	0.6670586750042367	0.0010236194501343785
38	1.1373549445937539E-4	2.2747098891875078E-4	0.0	0.9007851161182531	0.0010236194501343785
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1335	0.0	20.109314	1
ACTAGAC	55	0.0044828234	19.999872	3
TAGGACG	225	0.0	19.555431	4
GTAAACG	200	3.6379788E-12	17.599886	27
CGCGTAA	140	8.3649866E-8	17.285603	10
GCGTAAC	140	8.3649866E-8	17.285603	11
GTATAGA	105	3.765314E-5	16.76561	1
CGTGCGT	80	0.0019878554	16.499893	9
CGTCTTA	150	1.8360333E-7	16.133228	15
GTATTAG	315	0.0	16.067045	1
CGCTTCG	235	3.6379788E-12	15.91479	32
TAACGGC	195	8.894858E-10	15.79477	36
GAGCGAA	405	0.0	15.752984	16
AACCGCG	270	0.0	15.48138	7
TACCGGC	100	4.939893E-4	15.400776	2
GCGCCGA	300	0.0	15.3999	19
GTATAGC	115	8.19799E-5	15.307732	1
GGCGTTA	230	3.6379788E-11	15.304249	42
TAAACGC	230	3.6379788E-11	15.304249	28
AACGCAG	1745	0.0	15.128842	6
>>END_MODULE